Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF2246

Experiment: LB_plus_SM_buffer with Ffm_phage 0.1875 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF2243 and GFF2244 are separated by 14 nucleotidesGFF2244 and GFF2245 are separated by 14 nucleotidesGFF2245 and GFF2246 are separated by 85 nucleotidesGFF2246 and GFF2247 are separated by 193 nucleotidesGFF2247 and GFF2248 overlap by 30 nucleotidesGFF2248 and GFF2249 are separated by 0 nucleotidesGFF2249 and GFF2250 are separated by 46 nucleotides GFF2243 - GNAT family acetyltransferase YiiD potentially involved in tRNA processing, at 37,617 to 38,558 GFF2243 GFF2244 - FIG074102: hypothetical protein, at 38,573 to 39,001 GFF2244 GFF2245 - Sea40, at 39,016 to 39,327 GFF2245 GFF2246 - Lipase, GDXG family, at 39,413 to 40,342 GFF2246 GFF2247 - FIG01046414: hypothetical protein, at 40,536 to 40,769 GFF2247 GFF2248 - FIG00642734: hypothetical protein, at 40,740 to 40,871 GFF2248 GFF2249 - Putative merR family bacterial regulatory protein, at 40,872 to 41,162 GFF2249 GFF2250 - formate dehydrogenase formation protein FdhE, at 41,209 to 42,138 GFF2250 Position (kb) 39 40 41Strain fitness (log2 ratio) -2 -1 0 1at 38.507 kb on - strandat 38.567 kb on - strandat 38.598 kb on - strandat 38.614 kb on + strandat 38.626 kb on + strand, within GFF2244at 38.658 kb on - strand, within GFF2244at 38.658 kb on - strand, within GFF2244at 38.847 kb on - strand, within GFF2244at 38.969 kb on - strandat 39.036 kb on - strandat 39.038 kb on + strandat 39.038 kb on + strandat 39.038 kb on + strandat 39.075 kb on + strand, within GFF2245at 39.094 kb on - strand, within GFF2245at 39.170 kb on + strand, within GFF2245at 39.195 kb on + strand, within GFF2245at 39.281 kb on + strand, within GFF2245at 39.440 kb on + strandat 39.440 kb on + strandat 39.441 kb on - strandat 39.441 kb on - strandat 39.441 kb on - strandat 39.616 kb on + strand, within GFF2246at 39.617 kb on - strand, within GFF2246at 39.700 kb on + strand, within GFF2246at 39.702 kb on + strand, within GFF2246at 39.702 kb on + strand, within GFF2246at 39.702 kb on + strand, within GFF2246at 39.702 kb on + strand, within GFF2246at 39.702 kb on + strand, within GFF2246at 39.702 kb on + strand, within GFF2246at 39.703 kb on - strand, within GFF2246at 39.744 kb on + strand, within GFF2246at 39.744 kb on + strand, within GFF2246at 39.745 kb on - strand, within GFF2246at 39.765 kb on + strand, within GFF2246at 39.766 kb on - strand, within GFF2246at 39.766 kb on - strand, within GFF2246at 39.802 kb on - strand, within GFF2246at 39.802 kb on - strand, within GFF2246at 39.946 kb on + strand, within GFF2246at 39.950 kb on - strand, within GFF2246at 39.974 kb on - strand, within GFF2246at 40.011 kb on + strand, within GFF2246at 40.111 kb on - strand, within GFF2246at 40.114 kb on - strand, within GFF2246at 40.114 kb on - strand, within GFF2246at 40.186 kb on + strand, within GFF2246at 40.187 kb on - strand, within GFF2246at 40.344 kb on + strandat 40.344 kb on + strandat 40.344 kb on + strandat 40.485 kb on - strandat 40.504 kb on - strandat 40.505 kb on + strandat 40.624 kb on - strand, within GFF2247at 40.624 kb on - strand, within GFF2247at 40.629 kb on - strand, within GFF2247at 40.811 kb on + strand, within GFF2248at 40.850 kb on + strand, within GFF2248at 40.869 kb on + strandat 40.870 kb on - strandat 40.908 kb on - strand, within GFF2249at 40.956 kb on - strand, within GFF2249at 40.964 kb on + strand, within GFF2249at 41.107 kb on + strand, within GFF2249at 41.143 kb on + strandat 41.144 kb on - strandat 41.144 kb on - strandat 41.181 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 0.1875 MOI
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38,507 - -0.8
38,567 - -0.4
38,598 - +0.2
38,614 + -1.2
38,626 + GFF2244 0.12 +0.2
38,658 - GFF2244 0.20 +0.1
38,658 - GFF2244 0.20 +0.1
38,847 - GFF2244 0.64 -1.4
38,969 - +1.1
39,036 - +0.3
39,038 + -0.0
39,038 + +0.6
39,038 + -0.4
39,075 + GFF2245 0.19 +0.2
39,094 - GFF2245 0.25 +0.8
39,170 + GFF2245 0.49 -0.0
39,195 + GFF2245 0.57 +0.3
39,281 + GFF2245 0.85 +0.5
39,440 + -0.3
39,440 + +0.1
39,441 - -0.2
39,441 - +0.9
39,441 - -0.5
39,616 + GFF2246 0.22 -0.8
39,617 - GFF2246 0.22 +0.2
39,700 + GFF2246 0.31 -0.1
39,702 + GFF2246 0.31 +0.2
39,702 + GFF2246 0.31 -0.3
39,702 + GFF2246 0.31 +1.1
39,702 + GFF2246 0.31 -0.2
39,702 + GFF2246 0.31 +0.0
39,702 + GFF2246 0.31 -0.6
39,703 - GFF2246 0.31 +0.4
39,744 + GFF2246 0.36 -0.1
39,744 + GFF2246 0.36 -0.3
39,745 - GFF2246 0.36 -0.0
39,765 + GFF2246 0.38 +0.6
39,766 - GFF2246 0.38 +0.4
39,766 - GFF2246 0.38 +1.4
39,802 - GFF2246 0.42 +0.8
39,802 - GFF2246 0.42 +0.4
39,946 + GFF2246 0.57 -0.8
39,950 - GFF2246 0.58 -0.1
39,974 - GFF2246 0.60 -0.2
40,011 + GFF2246 0.64 -0.5
40,111 - GFF2246 0.75 -0.1
40,114 - GFF2246 0.75 +1.0
40,114 - GFF2246 0.75 +1.5
40,186 + GFF2246 0.83 +0.2
40,187 - GFF2246 0.83 +0.6
40,344 + -0.1
40,344 + +0.1
40,344 + +0.2
40,485 - -0.2
40,504 - -0.4
40,505 + -0.8
40,624 - GFF2247 0.38 +0.1
40,624 - GFF2247 0.38 +1.1
40,629 - GFF2247 0.40 -0.7
40,811 + GFF2248 0.54 +0.7
40,850 + GFF2248 0.83 -0.3
40,869 + +0.3
40,870 - -1.9
40,908 - GFF2249 0.12 -1.2
40,956 - GFF2249 0.29 -0.3
40,964 + GFF2249 0.32 -0.6
41,107 + GFF2249 0.81 -0.3
41,143 + -0.4
41,144 - -0.1
41,144 - -0.3
41,181 + -0.3

Or see this region's nucleotide sequence