Experiment: LB_plus_SM_buffer with Ffm_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2229 and GFF2230 are separated by 156 nucleotides GFF2230 and GFF2231 are separated by 67 nucleotides GFF2231 and GFF2232 are separated by 45 nucleotides
GFF2229 - FIG074102: hypothetical protein, at 22,929 to 23,777
GFF2229
GFF2230 - Outer membrane sugar transport protein YshA, at 23,934 to 24,626
GFF2230
GFF2231 - Glucuronide transport protein YihO, at 24,694 to 26,115
GFF2231
GFF2232 - Glucuronide transport protein YihP, homologous to YihO, at 26,161 to 27,543
GFF2232
Position (kb) 24
25
26
27 Strain fitness (log2 ratio) -1
0
1 at 23.760 kb on - strand at 23.805 kb on - strand at 24.236 kb on - strand, within GFF2230 at 24.451 kb on + strand, within GFF2230 at 24.510 kb on - strand, within GFF2230 at 24.586 kb on - strand at 24.911 kb on - strand, within GFF2231 at 24.943 kb on - strand, within GFF2231 at 24.994 kb on - strand, within GFF2231 at 25.113 kb on + strand, within GFF2231 at 25.126 kb on - strand, within GFF2231 at 25.203 kb on - strand, within GFF2231 at 25.247 kb on - strand, within GFF2231 at 25.356 kb on + strand, within GFF2231 at 25.439 kb on - strand, within GFF2231 at 25.475 kb on + strand, within GFF2231 at 25.476 kb on - strand, within GFF2231 at 25.553 kb on + strand, within GFF2231 at 25.553 kb on + strand, within GFF2231 at 25.554 kb on - strand, within GFF2231 at 25.554 kb on - strand, within GFF2231 at 25.576 kb on - strand, within GFF2231 at 25.603 kb on + strand, within GFF2231 at 25.604 kb on - strand, within GFF2231 at 25.608 kb on + strand, within GFF2231 at 25.609 kb on - strand, within GFF2231 at 25.630 kb on - strand, within GFF2231 at 25.706 kb on + strand, within GFF2231 at 25.706 kb on + strand, within GFF2231 at 25.786 kb on - strand, within GFF2231 at 25.786 kb on - strand, within GFF2231 at 25.848 kb on + strand, within GFF2231 at 25.920 kb on + strand, within GFF2231 at 25.967 kb on + strand, within GFF2231 at 25.967 kb on + strand, within GFF2231 at 25.968 kb on - strand, within GFF2231 at 26.006 kb on - strand at 26.010 kb on - strand at 26.010 kb on - strand at 26.207 kb on + strand at 26.208 kb on - strand at 26.248 kb on - strand at 26.280 kb on + strand at 26.573 kb on + strand, within GFF2232 at 26.574 kb on - strand, within GFF2232 at 26.574 kb on - strand, within GFF2232 at 26.603 kb on - strand, within GFF2232 at 26.735 kb on + strand, within GFF2232 at 26.744 kb on + strand, within GFF2232 at 26.747 kb on + strand, within GFF2232 at 26.788 kb on - strand, within GFF2232 at 26.811 kb on + strand, within GFF2232 at 26.855 kb on - strand, within GFF2232 at 26.855 kb on - strand, within GFF2232 at 26.931 kb on + strand, within GFF2232 at 26.936 kb on - strand, within GFF2232 at 26.975 kb on + strand, within GFF2232 at 27.030 kb on + strand, within GFF2232
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 0.1875 MOI remove 23,760 - -0.3 23,805 - +0.2 24,236 - GFF2230 0.44 -0.0 24,451 + GFF2230 0.75 -0.4 24,510 - GFF2230 0.83 +0.2 24,586 - +0.2 24,911 - GFF2231 0.15 +0.5 24,943 - GFF2231 0.18 +0.2 24,994 - GFF2231 0.21 -0.3 25,113 + GFF2231 0.29 +0.1 25,126 - GFF2231 0.30 -1.6 25,203 - GFF2231 0.36 +0.6 25,247 - GFF2231 0.39 +0.3 25,356 + GFF2231 0.47 -0.1 25,439 - GFF2231 0.52 -0.7 25,475 + GFF2231 0.55 -0.1 25,476 - GFF2231 0.55 +0.1 25,553 + GFF2231 0.60 +0.3 25,553 + GFF2231 0.60 -0.1 25,554 - GFF2231 0.60 -0.7 25,554 - GFF2231 0.60 -0.3 25,576 - GFF2231 0.62 -0.4 25,603 + GFF2231 0.64 +0.7 25,604 - GFF2231 0.64 -0.1 25,608 + GFF2231 0.64 -0.2 25,609 - GFF2231 0.64 -0.9 25,630 - GFF2231 0.66 +0.2 25,706 + GFF2231 0.71 +0.4 25,706 + GFF2231 0.71 -1.3 25,786 - GFF2231 0.77 +0.8 25,786 - GFF2231 0.77 -0.9 25,848 + GFF2231 0.81 -0.5 25,920 + GFF2231 0.86 -0.5 25,967 + GFF2231 0.90 -0.7 25,967 + GFF2231 0.90 -0.1 25,968 - GFF2231 0.90 -0.3 26,006 - +0.1 26,010 - +0.9 26,010 - -0.4 26,207 + -0.2 26,208 - +0.4 26,248 - +0.2 26,280 + +0.2 26,573 + GFF2232 0.30 +0.7 26,574 - GFF2232 0.30 +0.4 26,574 - GFF2232 0.30 +0.6 26,603 - GFF2232 0.32 -0.4 26,735 + GFF2232 0.42 -0.0 26,744 + GFF2232 0.42 -0.4 26,747 + GFF2232 0.42 +0.5 26,788 - GFF2232 0.45 +0.1 26,811 + GFF2232 0.47 +0.2 26,855 - GFF2232 0.50 +0.1 26,855 - GFF2232 0.50 +0.8 26,931 + GFF2232 0.56 +0.1 26,936 - GFF2232 0.56 +0.5 26,975 + GFF2232 0.59 -0.4 27,030 + GFF2232 0.63 +0.1
Or see this region's nucleotide sequence