Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF2226

Experiment: LB_plus_SM_buffer with Ffm_phage 0.1875 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF2224 and GFF2225 overlap by 16 nucleotidesGFF2225 and GFF2226 overlap by 4 nucleotidesGFF2226 and GFF2227 are separated by 1 nucleotidesGFF2227 and GFF2228 overlap by 8 nucleotides GFF2224 - Putative hydrolase, at 18,186 to 18,920 GFF2224 GFF2225 - FIG01046022: hypothetical protein, at 18,905 to 19,783 GFF2225 GFF2226 - Hypothetical radical SAM family enzyme in interesting gene cluster, at 19,780 to 21,021 GFF2226 GFF2227 - FIG01124638: hypothetical protein, at 21,023 to 21,898 GFF2227 GFF2228 - FIG00638667: hypothetical protein, at 21,891 to 22,877 GFF2228 Position (kb) 19 20 21 22Strain fitness (log2 ratio) -2 -1 0 1at 18.906 kb on + strandat 18.991 kb on - strandat 19.500 kb on - strand, within GFF2225at 19.560 kb on + strand, within GFF2225at 19.561 kb on - strand, within GFF2225at 19.563 kb on + strand, within GFF2225at 19.594 kb on + strand, within GFF2225at 19.595 kb on - strand, within GFF2225at 19.705 kb on - strandat 19.716 kb on + strandat 19.716 kb on + strandat 19.717 kb on - strandat 19.729 kb on + strandat 19.729 kb on + strandat 19.730 kb on - strandat 19.898 kb on + strandat 19.899 kb on - strandat 20.141 kb on + strand, within GFF2226at 20.304 kb on - strand, within GFF2226at 20.307 kb on - strand, within GFF2226at 20.311 kb on - strand, within GFF2226at 20.311 kb on - strand, within GFF2226at 20.311 kb on - strand, within GFF2226at 20.338 kb on - strand, within GFF2226at 20.526 kb on + strand, within GFF2226at 20.527 kb on - strand, within GFF2226at 20.527 kb on - strand, within GFF2226at 20.531 kb on - strand, within GFF2226at 20.562 kb on + strand, within GFF2226at 20.716 kb on + strand, within GFF2226at 20.807 kb on + strand, within GFF2226at 20.886 kb on + strand, within GFF2226at 20.895 kb on - strand, within GFF2226at 20.895 kb on - strand, within GFF2226at 20.973 kb on + strandat 20.992 kb on - strandat 20.994 kb on - strandat 21.015 kb on + strandat 21.022 kb on - strandat 21.054 kb on + strandat 21.096 kb on + strandat 21.096 kb on + strandat 21.207 kb on + strand, within GFF2227at 21.207 kb on + strand, within GFF2227at 21.208 kb on - strand, within GFF2227at 21.303 kb on + strand, within GFF2227at 21.304 kb on - strand, within GFF2227at 21.388 kb on + strand, within GFF2227at 21.485 kb on - strand, within GFF2227at 21.625 kb on + strand, within GFF2227at 21.712 kb on - strand, within GFF2227at 21.787 kb on + strand, within GFF2227at 21.812 kb on - strandat 22.016 kb on + strand, within GFF2228

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 0.1875 MOI
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18,906 + +0.2
18,991 - +0.1
19,500 - GFF2225 0.68 -0.4
19,560 + GFF2225 0.75 -0.6
19,561 - GFF2225 0.75 -0.2
19,563 + GFF2225 0.75 +0.7
19,594 + GFF2225 0.78 -2.1
19,595 - GFF2225 0.78 +0.7
19,705 - +0.9
19,716 + -1.6
19,716 + -0.1
19,717 - -0.1
19,729 + +0.9
19,729 + +0.4
19,730 - -0.2
19,898 + -0.0
19,899 - -0.1
20,141 + GFF2226 0.29 +0.6
20,304 - GFF2226 0.42 -0.1
20,307 - GFF2226 0.42 +0.1
20,311 - GFF2226 0.43 +0.0
20,311 - GFF2226 0.43 +0.2
20,311 - GFF2226 0.43 +0.0
20,338 - GFF2226 0.45 -0.4
20,526 + GFF2226 0.60 -0.9
20,527 - GFF2226 0.60 -0.1
20,527 - GFF2226 0.60 -0.3
20,531 - GFF2226 0.60 -0.7
20,562 + GFF2226 0.63 -0.6
20,716 + GFF2226 0.75 -0.0
20,807 + GFF2226 0.83 -0.2
20,886 + GFF2226 0.89 -1.1
20,895 - GFF2226 0.90 +0.6
20,895 - GFF2226 0.90 -0.6
20,973 + -0.1
20,992 - +0.2
20,994 - -0.4
21,015 + +0.6
21,022 - -0.3
21,054 + +0.1
21,096 + +0.2
21,096 + -0.3
21,207 + GFF2227 0.21 +0.7
21,207 + GFF2227 0.21 +0.3
21,208 - GFF2227 0.21 -1.1
21,303 + GFF2227 0.32 +0.2
21,304 - GFF2227 0.32 +0.0
21,388 + GFF2227 0.42 +0.4
21,485 - GFF2227 0.53 -0.5
21,625 + GFF2227 0.69 -0.2
21,712 - GFF2227 0.79 -0.4
21,787 + GFF2227 0.87 -0.0
21,812 - +0.7
22,016 + GFF2228 0.13 -0.1

Or see this region's nucleotide sequence