Experiment: LB_plus_SM_buffer with Ffm_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1845 and GFF1846 overlap by 11 nucleotides GFF1846 and GFF1847 overlap by 4 nucleotides
GFF1845 - Anaerobic glycerol-3-phosphate dehydrogenase subunit A (EC 1.1.5.3), at 36,629 to 38,257
GFF1845
GFF1846 - Anaerobic glycerol-3-phosphate dehydrogenase subunit B (EC 1.1.5.3), at 38,247 to 39,506
GFF1846
GFF1847 - Anaerobic glycerol-3-phosphate dehydrogenase subunit C (EC 1.1.5.3), at 39,503 to 40,693
GFF1847
Position (kb) 38
39
40 Strain fitness (log2 ratio) -1
0
1 at 37.451 kb on + strand, within GFF1845 at 37.673 kb on - strand, within GFF1845 at 38.154 kb on + strand at 38.155 kb on - strand at 38.155 kb on - strand at 38.155 kb on - strand at 38.155 kb on - strand at 38.267 kb on + strand at 38.472 kb on + strand, within GFF1846 at 38.502 kb on - strand, within GFF1846 at 38.531 kb on + strand, within GFF1846 at 38.619 kb on - strand, within GFF1846 at 38.779 kb on + strand, within GFF1846 at 38.860 kb on - strand, within GFF1846 at 38.878 kb on + strand, within GFF1846 at 39.170 kb on - strand, within GFF1846 at 39.279 kb on + strand, within GFF1846 at 39.382 kb on - strand at 39.388 kb on + strand at 39.389 kb on - strand at 39.389 kb on - strand at 39.418 kb on + strand at 39.418 kb on + strand at 39.418 kb on + strand at 39.419 kb on - strand at 39.531 kb on + strand at 39.674 kb on - strand, within GFF1847 at 39.724 kb on + strand, within GFF1847 at 40.135 kb on + strand, within GFF1847 at 40.204 kb on + strand, within GFF1847 at 40.234 kb on + strand, within GFF1847 at 40.235 kb on - strand, within GFF1847 at 40.301 kb on - strand, within GFF1847 at 40.385 kb on + strand, within GFF1847 at 40.385 kb on + strand, within GFF1847 at 40.385 kb on + strand, within GFF1847 at 40.391 kb on - strand, within GFF1847 at 40.473 kb on + strand, within GFF1847
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 0.1875 MOI remove 37,451 + GFF1845 0.50 -1.0 37,673 - GFF1845 0.64 +0.1 38,154 + +0.2 38,155 - +0.7 38,155 - -0.0 38,155 - +0.1 38,155 - +0.1 38,267 + -0.4 38,472 + GFF1846 0.18 +0.3 38,502 - GFF1846 0.20 +0.3 38,531 + GFF1846 0.23 +0.6 38,619 - GFF1846 0.30 +1.0 38,779 + GFF1846 0.42 +0.3 38,860 - GFF1846 0.49 +0.1 38,878 + GFF1846 0.50 -0.1 39,170 - GFF1846 0.73 +0.4 39,279 + GFF1846 0.82 -1.6 39,382 - -1.0 39,388 + +0.1 39,389 - -0.3 39,389 - +0.0 39,418 + -0.3 39,418 + +1.0 39,418 + -0.4 39,419 - +0.3 39,531 + +0.1 39,674 - GFF1847 0.14 +0.7 39,724 + GFF1847 0.19 -0.2 40,135 + GFF1847 0.53 +0.1 40,204 + GFF1847 0.59 +0.0 40,234 + GFF1847 0.61 +0.4 40,235 - GFF1847 0.61 +0.3 40,301 - GFF1847 0.67 +0.2 40,385 + GFF1847 0.74 +0.9 40,385 + GFF1847 0.74 +0.5 40,385 + GFF1847 0.74 +0.1 40,391 - GFF1847 0.75 -1.0 40,473 + GFF1847 0.81 +0.2
Or see this region's nucleotide sequence