Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF1819

Experiment: LB_plus_SM_buffer with Ffm_phage 0.1875 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1817 and GFF1818 are separated by 9 nucleotidesGFF1818 and GFF1819 are separated by 115 nucleotidesGFF1819 and GFF1820 overlap by 14 nucleotidesGFF1820 and GFF1821 are separated by 6 nucleotides GFF1817 - Cytochrome c-type protein NapC, at 76 to 678 GFF1817 GFF1818 - Nitrate reductase cytochrome c550-type subunit, at 688 to 1,137 GFF1818 GFF1819 - Polyferredoxin NapH (periplasmic nitrate reductase), at 1,253 to 2,122 GFF1819 GFF1820 - Ferredoxin-type protein NapG (periplasmic nitrate reductase), at 2,109 to 2,804 GFF1820 GFF1821 - Periplasmic nitrate reductase precursor (EC 1.7.99.4), at 2,811 to 5,297 GFF1821 Position (kb) 1 2 3Strain fitness (log2 ratio) -1 0 1at 0.322 kb on - strand, within GFF1817at 0.467 kb on + strand, within GFF1817at 0.525 kb on + strand, within GFF1817at 0.720 kb on + strandat 0.721 kb on - strandat 0.755 kb on - strand, within GFF1818at 0.808 kb on - strand, within GFF1818at 0.964 kb on + strand, within GFF1818at 1.076 kb on - strand, within GFF1818at 1.166 kb on - strandat 1.227 kb on - strandat 1.231 kb on - strandat 1.444 kb on + strand, within GFF1819at 1.494 kb on - strand, within GFF1819at 1.526 kb on - strand, within GFF1819at 1.625 kb on + strand, within GFF1819at 1.708 kb on - strand, within GFF1819at 1.711 kb on + strand, within GFF1819at 1.712 kb on + strand, within GFF1819at 1.713 kb on - strand, within GFF1819at 1.713 kb on - strand, within GFF1819at 1.713 kb on - strand, within GFF1819at 1.713 kb on - strand, within GFF1819at 1.715 kb on - strand, within GFF1819at 1.791 kb on - strand, within GFF1819at 1.955 kb on - strand, within GFF1819at 1.955 kb on - strand, within GFF1819at 1.963 kb on - strand, within GFF1819at 1.963 kb on - strand, within GFF1819at 1.963 kb on - strand, within GFF1819at 2.043 kb on - strandat 2.161 kb on - strandat 2.197 kb on - strand, within GFF1820at 2.804 kb on + strandat 2.813 kb on - strandat 2.828 kb on + strandat 2.926 kb on + strandat 2.926 kb on + strandat 2.926 kb on + strandat 2.926 kb on + strandat 2.927 kb on - strandat 2.927 kb on - strandat 2.927 kb on - strandat 2.927 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 0.1875 MOI
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322 - GFF1817 0.41 -0.2
467 + GFF1817 0.65 +0.1
525 + GFF1817 0.74 -1.0
720 + +0.3
721 - +0.1
755 - GFF1818 0.15 +0.1
808 - GFF1818 0.27 +0.2
964 + GFF1818 0.61 +0.6
1,076 - GFF1818 0.86 +0.1
1,166 - -0.2
1,227 - +0.5
1,231 - +0.5
1,444 + GFF1819 0.22 +0.5
1,494 - GFF1819 0.28 +0.3
1,526 - GFF1819 0.31 +0.1
1,625 + GFF1819 0.43 +0.2
1,708 - GFF1819 0.52 -0.0
1,711 + GFF1819 0.53 +0.5
1,712 + GFF1819 0.53 +0.7
1,713 - GFF1819 0.53 +0.1
1,713 - GFF1819 0.53 -0.4
1,713 - GFF1819 0.53 +0.1
1,713 - GFF1819 0.53 -0.1
1,715 - GFF1819 0.53 +0.1
1,791 - GFF1819 0.62 -0.2
1,955 - GFF1819 0.81 -0.0
1,955 - GFF1819 0.81 +0.7
1,963 - GFF1819 0.82 +0.4
1,963 - GFF1819 0.82 +0.6
1,963 - GFF1819 0.82 +0.6
2,043 - +0.4
2,161 - +0.4
2,197 - GFF1820 0.13 +1.1
2,804 + +0.4
2,813 - -0.1
2,828 + +0.3
2,926 + -0.0
2,926 + +1.0
2,926 + -1.0
2,926 + -0.3
2,927 - -0.7
2,927 - +0.6
2,927 - -0.4
2,927 - -0.5

Or see this region's nucleotide sequence