Experiment: LB_plus_SM_buffer with Ffm_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1802 and GFF1803 are separated by 158 nucleotides GFF1803 and GFF1804 are separated by 110 nucleotides
GFF1802 - Outer membrane protein N precursor, at 22,975 to 24,108
GFF1802
GFF1803 - Exoenzymes regulatory protein AepA precursor, at 24,267 to 25,967
GFF1803
GFF1804 - Sensory histidine kinase in two-component regulatory system with RstA, at 26,078 to 27,379
GFF1804
Position (kb) 24
25
26 Strain fitness (log2 ratio) -1
0
1 at 23.316 kb on + strand, within GFF1802 at 23.383 kb on + strand, within GFF1802 at 23.383 kb on + strand, within GFF1802 at 23.389 kb on + strand, within GFF1802 at 23.600 kb on - strand, within GFF1802 at 23.671 kb on + strand, within GFF1802 at 23.692 kb on - strand, within GFF1802 at 23.730 kb on + strand, within GFF1802 at 23.731 kb on - strand, within GFF1802 at 23.772 kb on + strand, within GFF1802 at 23.896 kb on - strand, within GFF1802 at 23.974 kb on + strand, within GFF1802 at 24.265 kb on + strand at 24.384 kb on - strand at 24.500 kb on + strand, within GFF1803 at 24.512 kb on - strand, within GFF1803 at 24.518 kb on + strand, within GFF1803 at 24.773 kb on + strand, within GFF1803 at 24.773 kb on + strand, within GFF1803 at 24.773 kb on + strand, within GFF1803 at 24.774 kb on - strand, within GFF1803 at 24.774 kb on - strand, within GFF1803 at 24.787 kb on - strand, within GFF1803 at 24.793 kb on - strand, within GFF1803 at 24.817 kb on + strand, within GFF1803 at 24.824 kb on - strand, within GFF1803 at 24.824 kb on - strand, within GFF1803 at 25.066 kb on + strand, within GFF1803 at 25.363 kb on - strand, within GFF1803 at 25.408 kb on + strand, within GFF1803 at 25.408 kb on + strand, within GFF1803 at 25.509 kb on + strand, within GFF1803 at 25.510 kb on - strand, within GFF1803 at 25.510 kb on - strand, within GFF1803 at 25.699 kb on + strand, within GFF1803 at 25.700 kb on - strand, within GFF1803 at 25.770 kb on - strand, within GFF1803 at 25.865 kb on + strand at 25.865 kb on + strand at 26.051 kb on + strand at 26.219 kb on + strand, within GFF1804 at 26.323 kb on - strand, within GFF1804 at 26.332 kb on + strand, within GFF1804 at 26.332 kb on + strand, within GFF1804 at 26.396 kb on + strand, within GFF1804 at 26.404 kb on + strand, within GFF1804 at 26.405 kb on - strand, within GFF1804 at 26.499 kb on + strand, within GFF1804 at 26.692 kb on + strand, within GFF1804 at 26.830 kb on + strand, within GFF1804
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 0.1875 MOI remove 23,316 + GFF1802 0.30 +1.1 23,383 + GFF1802 0.36 -0.1 23,383 + GFF1802 0.36 +0.7 23,389 + GFF1802 0.37 -0.3 23,600 - GFF1802 0.55 -0.7 23,671 + GFF1802 0.61 +0.8 23,692 - GFF1802 0.63 -0.4 23,730 + GFF1802 0.67 +1.1 23,731 - GFF1802 0.67 +0.3 23,772 + GFF1802 0.70 -0.3 23,896 - GFF1802 0.81 -0.1 23,974 + GFF1802 0.88 +0.5 24,265 + -0.5 24,384 - -0.1 24,500 + GFF1803 0.14 +0.5 24,512 - GFF1803 0.14 -1.2 24,518 + GFF1803 0.15 -0.1 24,773 + GFF1803 0.30 +0.1 24,773 + GFF1803 0.30 -0.8 24,773 + GFF1803 0.30 -0.7 24,774 - GFF1803 0.30 -0.2 24,774 - GFF1803 0.30 -1.7 24,787 - GFF1803 0.31 -0.3 24,793 - GFF1803 0.31 -0.1 24,817 + GFF1803 0.32 -1.1 24,824 - GFF1803 0.33 -0.6 24,824 - GFF1803 0.33 -0.1 25,066 + GFF1803 0.47 +0.2 25,363 - GFF1803 0.64 -0.1 25,408 + GFF1803 0.67 +0.4 25,408 + GFF1803 0.67 +0.2 25,509 + GFF1803 0.73 +0.0 25,510 - GFF1803 0.73 -0.2 25,510 - GFF1803 0.73 -0.0 25,699 + GFF1803 0.84 -0.1 25,700 - GFF1803 0.84 -0.3 25,770 - GFF1803 0.88 -0.7 25,865 + -0.3 25,865 + +0.5 26,051 + +0.0 26,219 + GFF1804 0.11 +0.9 26,323 - GFF1804 0.19 +0.1 26,332 + GFF1804 0.20 +0.3 26,332 + GFF1804 0.20 -0.9 26,396 + GFF1804 0.24 +0.4 26,404 + GFF1804 0.25 -0.1 26,405 - GFF1804 0.25 +0.4 26,499 + GFF1804 0.32 -0.4 26,692 + GFF1804 0.47 -0.4 26,830 + GFF1804 0.58 +0.5
Or see this region's nucleotide sequence