Experiment: LB_plus_SM_buffer with Ffm_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1763 and GFF1764 overlap by 17 nucleotides GFF1764 and GFF1765 overlap by 4 nucleotides GFF1765 and GFF1766 are separated by 123 nucleotides
GFF1763 - 2-dehydro-3-deoxygalactonokinase (EC 2.7.1.58), at 66,581 to 67,459
GFF1763
GFF1764 - 2-dehydro-3-deoxyphosphogalactonate aldolase (EC 4.1.2.21), at 67,443 to 68,060
GFF1764
GFF1765 - Galactonate dehydratase (EC 4.2.1.6), at 68,057 to 69,205
GFF1765
GFF1766 - D-galactonate transporter, at 69,329 to 70,627
GFF1766
Position (kb) 68
69
70 Strain fitness (log2 ratio) -1
0
1
2 at 67.166 kb on - strand, within GFF1763 at 67.356 kb on - strand, within GFF1763 at 67.369 kb on + strand, within GFF1763 at 67.369 kb on + strand, within GFF1763 at 67.458 kb on - strand at 67.475 kb on + strand at 67.480 kb on + strand at 67.487 kb on + strand at 67.488 kb on - strand at 67.547 kb on + strand, within GFF1764 at 67.549 kb on + strand, within GFF1764 at 67.550 kb on - strand, within GFF1764 at 67.609 kb on - strand, within GFF1764 at 67.642 kb on + strand, within GFF1764 at 67.660 kb on + strand, within GFF1764 at 67.661 kb on - strand, within GFF1764 at 67.740 kb on + strand, within GFF1764 at 67.741 kb on - strand, within GFF1764 at 67.741 kb on - strand, within GFF1764 at 68.054 kb on + strand at 68.081 kb on + strand at 68.082 kb on - strand at 68.088 kb on + strand at 68.174 kb on - strand, within GFF1765 at 68.190 kb on + strand, within GFF1765 at 68.191 kb on - strand, within GFF1765 at 68.191 kb on - strand, within GFF1765 at 68.208 kb on - strand, within GFF1765 at 68.253 kb on + strand, within GFF1765 at 68.254 kb on - strand, within GFF1765 at 68.254 kb on - strand, within GFF1765 at 68.406 kb on - strand, within GFF1765 at 68.406 kb on - strand, within GFF1765 at 68.408 kb on - strand, within GFF1765 at 68.678 kb on - strand, within GFF1765 at 68.765 kb on - strand, within GFF1765 at 68.765 kb on - strand, within GFF1765 at 68.765 kb on - strand, within GFF1765 at 68.861 kb on + strand, within GFF1765 at 68.888 kb on + strand, within GFF1765 at 68.888 kb on + strand, within GFF1765 at 68.889 kb on - strand, within GFF1765 at 69.005 kb on + strand, within GFF1765 at 69.007 kb on + strand, within GFF1765 at 69.007 kb on + strand, within GFF1765 at 69.008 kb on - strand, within GFF1765 at 69.105 kb on + strand at 69.136 kb on + strand at 69.136 kb on + strand at 69.161 kb on - strand at 69.260 kb on + strand at 69.261 kb on - strand at 69.340 kb on + strand at 69.401 kb on - strand at 69.401 kb on - strand at 69.401 kb on - strand at 69.401 kb on - strand at 69.419 kb on + strand at 69.525 kb on - strand, within GFF1766 at 69.525 kb on - strand, within GFF1766 at 69.566 kb on - strand, within GFF1766 at 69.622 kb on - strand, within GFF1766 at 69.724 kb on + strand, within GFF1766 at 69.768 kb on - strand, within GFF1766 at 69.878 kb on - strand, within GFF1766 at 69.934 kb on + strand, within GFF1766 at 69.953 kb on + strand, within GFF1766 at 69.953 kb on + strand, within GFF1766 at 69.954 kb on - strand, within GFF1766 at 70.054 kb on + strand, within GFF1766 at 70.055 kb on - strand, within GFF1766 at 70.096 kb on + strand, within GFF1766 at 70.127 kb on - strand, within GFF1766 at 70.186 kb on - strand, within GFF1766
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 0.1875 MOI remove 67,166 - GFF1763 0.67 -0.3 67,356 - GFF1763 0.88 +0.2 67,369 + GFF1763 0.90 +1.0 67,369 + GFF1763 0.90 +0.6 67,458 - +0.8 67,475 + -0.1 67,480 + +0.5 67,487 + -0.3 67,488 - +0.8 67,547 + GFF1764 0.17 +0.4 67,549 + GFF1764 0.17 +0.3 67,550 - GFF1764 0.17 +0.0 67,609 - GFF1764 0.27 +0.3 67,642 + GFF1764 0.32 +0.0 67,660 + GFF1764 0.35 +1.2 67,661 - GFF1764 0.35 +0.5 67,740 + GFF1764 0.48 +0.5 67,741 - GFF1764 0.48 +0.8 67,741 - GFF1764 0.48 -1.3 68,054 + -0.6 68,081 + -0.4 68,082 - -0.9 68,088 + +0.3 68,174 - GFF1765 0.10 -0.1 68,190 + GFF1765 0.12 +0.4 68,191 - GFF1765 0.12 +0.7 68,191 - GFF1765 0.12 -0.3 68,208 - GFF1765 0.13 +0.2 68,253 + GFF1765 0.17 +0.6 68,254 - GFF1765 0.17 +0.8 68,254 - GFF1765 0.17 -0.1 68,406 - GFF1765 0.30 +0.2 68,406 - GFF1765 0.30 -0.3 68,408 - GFF1765 0.31 +0.3 68,678 - GFF1765 0.54 +0.9 68,765 - GFF1765 0.62 -0.5 68,765 - GFF1765 0.62 +0.0 68,765 - GFF1765 0.62 +2.1 68,861 + GFF1765 0.70 +1.1 68,888 + GFF1765 0.72 -0.1 68,888 + GFF1765 0.72 -0.3 68,889 - GFF1765 0.72 +0.1 69,005 + GFF1765 0.83 -0.5 69,007 + GFF1765 0.83 +0.0 69,007 + GFF1765 0.83 -0.1 69,008 - GFF1765 0.83 +0.3 69,105 + +0.4 69,136 + +0.4 69,136 + +0.3 69,161 - +0.1 69,260 + -0.1 69,261 - -0.3 69,340 + -0.2 69,401 - -0.2 69,401 - +0.4 69,401 - -0.3 69,401 - -0.6 69,419 + +0.1 69,525 - GFF1766 0.15 +0.1 69,525 - GFF1766 0.15 -0.8 69,566 - GFF1766 0.18 -0.3 69,622 - GFF1766 0.23 +0.4 69,724 + GFF1766 0.30 -0.9 69,768 - GFF1766 0.34 +0.1 69,878 - GFF1766 0.42 +0.1 69,934 + GFF1766 0.47 -0.3 69,953 + GFF1766 0.48 -0.2 69,953 + GFF1766 0.48 -0.2 69,954 - GFF1766 0.48 +0.2 70,054 + GFF1766 0.56 +1.0 70,055 - GFF1766 0.56 +0.0 70,096 + GFF1766 0.59 +0.2 70,127 - GFF1766 0.61 -1.4 70,186 - GFF1766 0.66 +0.2
Or see this region's nucleotide sequence