Experiment: LB_plus_SM_buffer with Ffm_phage 0.1875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1235 and GFF1236 are separated by 419 nucleotides GFF1236 and GFF1237 are separated by 51 nucleotides GFF1237 and GFF1238 overlap by 38 nucleotides
GFF1235 - 27.5 kDa virulence protein, at 67,401 to 67,988
GFF1235
GFF1236 - Actin-ADP-ribosyltransferase, toxin SpvB, at 68,408 to 70,183
GFF1236
GFF1237 - FIG01046247: hypothetical protein, at 70,235 to 70,402
GFF1237
GFF1238 - 28.1 kDa virulence protein, at 70,365 to 71,132
GFF1238
Position (kb)
68
69
70
71 Strain fitness (log2 ratio)
-1
0
1
2 at 67.488 kb on - strand, within GFF1235 at 67.488 kb on - strand, within GFF1235 at 67.590 kb on - strand, within GFF1235 at 67.624 kb on + strand, within GFF1235 at 67.914 kb on - strand, within GFF1235 at 68.115 kb on - strand at 68.687 kb on - strand, within GFF1236 at 69.052 kb on + strand, within GFF1236 at 69.052 kb on + strand, within GFF1236 at 69.091 kb on + strand, within GFF1236 at 69.091 kb on + strand, within GFF1236 at 69.091 kb on + strand, within GFF1236 at 69.129 kb on + strand, within GFF1236 at 69.130 kb on - strand, within GFF1236 at 69.130 kb on - strand, within GFF1236 at 69.181 kb on - strand, within GFF1236 at 69.291 kb on + strand, within GFF1236 at 69.295 kb on + strand, within GFF1236 at 69.297 kb on + strand, within GFF1236 at 69.297 kb on + strand, within GFF1236 at 69.369 kb on - strand, within GFF1236 at 69.369 kb on - strand, within GFF1236 at 69.396 kb on - strand, within GFF1236 at 69.441 kb on + strand, within GFF1236 at 69.471 kb on + strand, within GFF1236 at 69.476 kb on + strand, within GFF1236 at 69.477 kb on - strand, within GFF1236 at 69.477 kb on - strand, within GFF1236 at 69.561 kb on + strand, within GFF1236 at 69.562 kb on - strand, within GFF1236 at 69.570 kb on + strand, within GFF1236 at 69.570 kb on + strand, within GFF1236 at 69.570 kb on + strand, within GFF1236 at 69.570 kb on + strand, within GFF1236 at 69.575 kb on - strand, within GFF1236 at 69.691 kb on + strand, within GFF1236 at 69.692 kb on - strand, within GFF1236 at 69.795 kb on + strand, within GFF1236 at 69.812 kb on + strand, within GFF1236 at 69.812 kb on + strand, within GFF1236 at 69.918 kb on + strand, within GFF1236 at 69.919 kb on - strand, within GFF1236 at 70.011 kb on + strand at 70.071 kb on - strand at 70.072 kb on + strand at 70.073 kb on - strand at 70.073 kb on - strand at 70.073 kb on - strand at 70.080 kb on - strand at 70.172 kb on + strand at 70.172 kb on + strand at 70.172 kb on + strand at 70.175 kb on + strand at 70.183 kb on + strand at 70.183 kb on + strand at 70.183 kb on + strand at 70.183 kb on + strand at 70.183 kb on + strand at 70.183 kb on + strand at 70.184 kb on - strand at 70.184 kb on - strand at 70.184 kb on - strand at 70.184 kb on - strand at 70.184 kb on - strand at 70.184 kb on - strand at 70.188 kb on + strand at 70.188 kb on + strand at 70.189 kb on - strand at 70.189 kb on - strand at 70.226 kb on - strand at 70.236 kb on + strand at 70.237 kb on - strand at 70.237 kb on - strand at 70.346 kb on - strand, within GFF1237 at 70.346 kb on - strand, within GFF1237 at 70.367 kb on - strand at 70.424 kb on + strand at 70.432 kb on - strand at 70.469 kb on + strand, within GFF1238 at 70.505 kb on - strand, within GFF1238 at 70.597 kb on + strand, within GFF1238 at 70.597 kb on + strand, within GFF1238 at 70.598 kb on - strand, within GFF1238 at 70.598 kb on - strand, within GFF1238 at 70.601 kb on + strand, within GFF1238 at 70.601 kb on + strand, within GFF1238 at 70.601 kb on + strand, within GFF1238 at 70.601 kb on + strand, within GFF1238 at 70.601 kb on + strand, within GFF1238 at 70.602 kb on - strand, within GFF1238 at 70.602 kb on - strand, within GFF1238 at 70.602 kb on - strand, within GFF1238 at 70.602 kb on - strand, within GFF1238 at 70.664 kb on + strand, within GFF1238 at 70.665 kb on - strand, within GFF1238 at 70.665 kb on - strand, within GFF1238 at 70.701 kb on + strand, within GFF1238 at 70.729 kb on + strand, within GFF1238 at 70.927 kb on + strand, within GFF1238 at 70.928 kb on - strand, within GFF1238 at 70.989 kb on - strand, within GFF1238 at 70.989 kb on - strand, within GFF1238 at 71.132 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 0.1875 MOI remove 67,488 - GFF1235 0.15 -0.5 67,488 - GFF1235 0.15 +1.8 67,590 - GFF1235 0.32 -0.3 67,624 + GFF1235 0.38 +0.1 67,914 - GFF1235 0.87 -1.0 68,115 - +0.1 68,687 - GFF1236 0.16 +1.0 69,052 + GFF1236 0.36 +1.8 69,052 + GFF1236 0.36 -0.1 69,091 + GFF1236 0.38 +0.4 69,091 + GFF1236 0.38 +0.7 69,091 + GFF1236 0.38 +1.5 69,129 + GFF1236 0.41 +0.8 69,130 - GFF1236 0.41 +0.2 69,130 - GFF1236 0.41 +0.0 69,181 - GFF1236 0.44 -0.0 69,291 + GFF1236 0.50 +0.3 69,295 + GFF1236 0.50 -0.2 69,297 + GFF1236 0.50 -0.2 69,297 + GFF1236 0.50 +0.1 69,369 - GFF1236 0.54 +0.1 69,369 - GFF1236 0.54 -0.2 69,396 - GFF1236 0.56 -0.3 69,441 + GFF1236 0.58 +0.3 69,471 + GFF1236 0.60 +0.3 69,476 + GFF1236 0.60 -0.0 69,477 - GFF1236 0.60 +0.2 69,477 - GFF1236 0.60 -0.3 69,561 + GFF1236 0.65 -0.1 69,562 - GFF1236 0.65 +0.2 69,570 + GFF1236 0.65 +0.8 69,570 + GFF1236 0.65 +0.0 69,570 + GFF1236 0.65 +0.1 69,570 + GFF1236 0.65 -0.4 69,575 - GFF1236 0.66 +0.1 69,691 + GFF1236 0.72 +0.2 69,692 - GFF1236 0.72 +0.7 69,795 + GFF1236 0.78 +0.3 69,812 + GFF1236 0.79 +0.1 69,812 + GFF1236 0.79 +0.3 69,918 + GFF1236 0.85 +0.4 69,919 - GFF1236 0.85 +0.1 70,011 + -0.3 70,071 - +0.7 70,072 + +0.6 70,073 - -0.2 70,073 - +0.5 70,073 - +0.0 70,080 - +1.3 70,172 + +0.6 70,172 + +0.3 70,172 + -0.0 70,175 + -0.4 70,183 + -0.9 70,183 + +0.0 70,183 + -0.5 70,183 + +0.3 70,183 + -0.2 70,183 + +0.4 70,184 - -0.2 70,184 - +0.3 70,184 - +0.2 70,184 - +0.0 70,184 - -0.0 70,184 - -0.4 70,188 + +0.2 70,188 + -0.3 70,189 - +0.1 70,189 - -0.1 70,226 - -0.1 70,236 + +0.1 70,237 - +0.3 70,237 - -0.4 70,346 - GFF1237 0.66 -1.2 70,346 - GFF1237 0.66 +0.2 70,367 - +0.0 70,424 + -0.1 70,432 - -0.1 70,469 + GFF1238 0.14 -0.1 70,505 - GFF1238 0.18 -0.1 70,597 + GFF1238 0.30 -0.7 70,597 + GFF1238 0.30 +0.7 70,598 - GFF1238 0.30 +0.4 70,598 - GFF1238 0.30 -0.1 70,601 + GFF1238 0.31 -0.4 70,601 + GFF1238 0.31 -0.1 70,601 + GFF1238 0.31 +0.4 70,601 + GFF1238 0.31 +0.4 70,601 + GFF1238 0.31 +0.6 70,602 - GFF1238 0.31 -0.3 70,602 - GFF1238 0.31 +0.3 70,602 - GFF1238 0.31 +0.1 70,602 - GFF1238 0.31 -1.0 70,664 + GFF1238 0.39 -1.0 70,665 - GFF1238 0.39 -0.2 70,665 - GFF1238 0.39 +0.4 70,701 + GFF1238 0.44 +0.8 70,729 + GFF1238 0.47 +0.5 70,927 + GFF1238 0.73 -0.1 70,928 - GFF1238 0.73 -0.3 70,989 - GFF1238 0.81 +0.7 70,989 - GFF1238 0.81 -1.6 71,132 + -0.2
Or see this region's nucleotide sequence