Experiment: LB_plus_SM_buffer with Ffm_phage 1.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF963 and GFF964 overlap by 1 nucleotides GFF964 and GFF965 are separated by 45 nucleotides
GFF963 - putative ABC-type sugar/spermidine/putrescine transport system ATPase component, at 145,523 to 146,614
GFF963
GFF964 - putative type II restriction enzyme methylase subunit, at 146,614 to 150,291
GFF964
GFF965 - ATPase-like protein, at 150,337 to 153,978
GFF965
Position (kb) 146
147
148
149
150
151 Strain fitness (log2 ratio) -2
-1
0
1 at 145.722 kb on - strand, within GFF963 at 145.722 kb on - strand, within GFF963 at 145.740 kb on - strand, within GFF963 at 145.802 kb on + strand, within GFF963 at 145.802 kb on + strand, within GFF963 at 145.978 kb on + strand, within GFF963 at 145.992 kb on + strand, within GFF963 at 145.993 kb on - strand, within GFF963 at 145.993 kb on - strand, within GFF963 at 145.993 kb on - strand, within GFF963 at 146.096 kb on - strand, within GFF963 at 146.123 kb on + strand, within GFF963 at 146.124 kb on - strand, within GFF963 at 146.124 kb on - strand, within GFF963 at 146.144 kb on - strand, within GFF963 at 146.168 kb on - strand, within GFF963 at 146.169 kb on + strand, within GFF963 at 146.170 kb on - strand, within GFF963 at 146.170 kb on - strand, within GFF963 at 146.198 kb on - strand, within GFF963 at 146.198 kb on - strand, within GFF963 at 146.313 kb on - strand, within GFF963 at 146.395 kb on - strand, within GFF963 at 146.410 kb on + strand, within GFF963 at 146.619 kb on + strand at 146.619 kb on + strand at 146.620 kb on - strand at 146.685 kb on + strand at 146.713 kb on - strand at 146.733 kb on - strand at 146.733 kb on - strand at 146.890 kb on + strand at 146.890 kb on + strand at 146.895 kb on - strand at 146.931 kb on - strand at 146.990 kb on + strand, within GFF964 at 147.260 kb on + strand, within GFF964 at 147.261 kb on - strand, within GFF964 at 147.312 kb on - strand, within GFF964 at 147.312 kb on - strand, within GFF964 at 147.365 kb on + strand, within GFF964 at 147.380 kb on - strand, within GFF964 at 148.132 kb on - strand, within GFF964 at 148.423 kb on - strand, within GFF964 at 148.756 kb on - strand, within GFF964 at 148.756 kb on - strand, within GFF964 at 148.818 kb on - strand, within GFF964 at 149.015 kb on - strand, within GFF964 at 149.304 kb on + strand, within GFF964 at 149.400 kb on + strand, within GFF964 at 149.453 kb on + strand, within GFF964 at 149.628 kb on + strand, within GFF964 at 149.629 kb on - strand, within GFF964 at 149.630 kb on + strand, within GFF964 at 149.631 kb on - strand, within GFF964 at 149.631 kb on - strand, within GFF964 at 149.631 kb on - strand, within GFF964 at 149.744 kb on - strand, within GFF964 at 149.890 kb on + strand, within GFF964 at 150.003 kb on - strand at 150.003 kb on - strand at 150.138 kb on - strand at 150.387 kb on + strand at 150.521 kb on - strand at 150.530 kb on + strand at 150.530 kb on + strand at 150.530 kb on + strand at 150.530 kb on + strand at 150.531 kb on - strand at 150.531 kb on - strand at 150.531 kb on - strand at 150.577 kb on + strand at 150.622 kb on - strand at 150.622 kb on - strand at 150.845 kb on - strand, within GFF965 at 150.903 kb on + strand, within GFF965 at 150.904 kb on - strand, within GFF965 at 150.905 kb on + strand, within GFF965 at 150.906 kb on - strand, within GFF965 at 150.906 kb on - strand, within GFF965 at 151.032 kb on + strand, within GFF965 at 151.191 kb on + strand, within GFF965
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 1.875 MOI remove 145,722 - GFF963 0.18 +0.9 145,722 - GFF963 0.18 +0.1 145,740 - GFF963 0.20 -0.4 145,802 + GFF963 0.26 -0.6 145,802 + GFF963 0.26 +0.6 145,978 + GFF963 0.42 +0.2 145,992 + GFF963 0.43 -0.3 145,993 - GFF963 0.43 -0.2 145,993 - GFF963 0.43 +0.2 145,993 - GFF963 0.43 -0.6 146,096 - GFF963 0.52 -0.1 146,123 + GFF963 0.55 -0.9 146,124 - GFF963 0.55 +0.1 146,124 - GFF963 0.55 +0.0 146,144 - GFF963 0.57 -0.1 146,168 - GFF963 0.59 -0.2 146,169 + GFF963 0.59 +0.3 146,170 - GFF963 0.59 +0.1 146,170 - GFF963 0.59 -0.1 146,198 - GFF963 0.62 +0.3 146,198 - GFF963 0.62 -1.2 146,313 - GFF963 0.72 +0.7 146,395 - GFF963 0.80 -0.2 146,410 + GFF963 0.81 -0.0 146,619 + +0.3 146,619 + +0.0 146,620 - -0.1 146,685 + +0.0 146,713 - +0.2 146,733 - -0.2 146,733 - -0.6 146,890 + +0.2 146,890 + -0.4 146,895 - -0.1 146,931 - +0.1 146,990 + GFF964 0.10 -0.1 147,260 + GFF964 0.18 -0.5 147,261 - GFF964 0.18 -0.2 147,312 - GFF964 0.19 -0.2 147,312 - GFF964 0.19 -0.4 147,365 + GFF964 0.20 -1.0 147,380 - GFF964 0.21 -0.2 148,132 - GFF964 0.41 -0.1 148,423 - GFF964 0.49 -0.2 148,756 - GFF964 0.58 +0.1 148,756 - GFF964 0.58 +0.0 148,818 - GFF964 0.60 -0.2 149,015 - GFF964 0.65 -0.3 149,304 + GFF964 0.73 -0.5 149,400 + GFF964 0.76 -2.0 149,453 + GFF964 0.77 -0.3 149,628 + GFF964 0.82 +0.8 149,629 - GFF964 0.82 -0.3 149,630 + GFF964 0.82 -0.1 149,631 - GFF964 0.82 -0.1 149,631 - GFF964 0.82 -0.7 149,631 - GFF964 0.82 +0.1 149,744 - GFF964 0.85 +0.5 149,890 + GFF964 0.89 -0.1 150,003 - -0.1 150,003 - +0.4 150,138 - -0.2 150,387 + -0.1 150,521 - +0.1 150,530 + -0.1 150,530 + +0.6 150,530 + +0.4 150,530 + -0.6 150,531 - +0.2 150,531 - -0.1 150,531 - -0.9 150,577 + +1.1 150,622 - -0.2 150,622 - -0.4 150,845 - GFF965 0.14 -0.4 150,903 + GFF965 0.16 -0.1 150,904 - GFF965 0.16 -0.6 150,905 + GFF965 0.16 +0.1 150,906 - GFF965 0.16 -0.6 150,906 - GFF965 0.16 +0.5 151,032 + GFF965 0.19 -0.1 151,191 + GFF965 0.23 +0.3
Or see this region's nucleotide sequence