Experiment: LB_plus_SM_buffer with Ffm_phage 1.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF718 and GFF719 are separated by 28 nucleotides GFF719 and GFF720 are separated by 393 nucleotides GFF720 and GFF721 are separated by 261 nucleotides
GFF718 - putative inner membrane protein, at 74,148 to 75,044
GFF718
GFF719 - HMP-PP hydrolase (pyridoxal phosphatase) Cof, detected in genetic screen for thiamin metabolic genes (PMID:15292217), at 75,073 to 75,603
GFF719
GFF720 - FIG01046262: hypothetical protein, at 75,997 to 77,262
GFF720
GFF721 - HMP-PP hydrolase (pyridoxal phosphatase) Cof, detected in genetic screen for thiamin metabolic genes (PMID:15292217), at 77,524 to 78,333
GFF721
Position (kb)
75
76
77
78 Strain fitness (log2 ratio)
-1
0
1
2 at 75.138 kb on + strand, within GFF719 at 75.139 kb on - strand, within GFF719 at 75.373 kb on - strand, within GFF719 at 75.552 kb on - strand at 75.615 kb on + strand at 75.744 kb on - strand at 75.746 kb on + strand at 75.746 kb on + strand at 75.846 kb on + strand at 75.911 kb on + strand at 76.007 kb on - strand at 76.007 kb on - strand at 76.012 kb on + strand at 76.076 kb on + strand at 76.181 kb on + strand, within GFF720 at 76.181 kb on + strand, within GFF720 at 76.403 kb on + strand, within GFF720 at 76.588 kb on - strand, within GFF720 at 76.716 kb on + strand, within GFF720 at 76.768 kb on + strand, within GFF720 at 76.907 kb on + strand, within GFF720 at 76.907 kb on + strand, within GFF720 at 76.907 kb on + strand, within GFF720 at 76.907 kb on + strand, within GFF720 at 77.073 kb on + strand, within GFF720 at 77.074 kb on - strand, within GFF720 at 77.112 kb on + strand, within GFF720 at 77.135 kb on - strand, within GFF720 at 77.262 kb on + strand at 77.263 kb on - strand at 77.263 kb on - strand at 77.520 kb on - strand at 77.520 kb on - strand at 77.524 kb on - strand at 77.584 kb on + strand at 77.595 kb on - strand at 77.632 kb on - strand, within GFF721 at 77.668 kb on + strand, within GFF721 at 77.741 kb on - strand, within GFF721 at 77.820 kb on + strand, within GFF721 at 77.860 kb on + strand, within GFF721 at 77.861 kb on - strand, within GFF721 at 77.931 kb on - strand, within GFF721 at 77.964 kb on + strand, within GFF721 at 78.023 kb on + strand, within GFF721 at 78.024 kb on - strand, within GFF721 at 78.096 kb on + strand, within GFF721 at 78.205 kb on + strand, within GFF721
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 1.875 MOI remove 75,138 + GFF719 0.12 +0.1 75,139 - GFF719 0.12 +0.3 75,373 - GFF719 0.56 +0.3 75,552 - +0.2 75,615 + +0.4 75,744 - +1.8 75,746 + -0.7 75,746 + -0.2 75,846 + -0.2 75,911 + -0.7 76,007 - -1.3 76,007 - -0.8 76,012 + -0.1 76,076 + -0.2 76,181 + GFF720 0.15 -0.5 76,181 + GFF720 0.15 -0.1 76,403 + GFF720 0.32 +0.1 76,588 - GFF720 0.47 +0.5 76,716 + GFF720 0.57 -0.3 76,768 + GFF720 0.61 -0.1 76,907 + GFF720 0.72 -0.2 76,907 + GFF720 0.72 +0.0 76,907 + GFF720 0.72 -0.4 76,907 + GFF720 0.72 -0.6 77,073 + GFF720 0.85 +0.6 77,074 - GFF720 0.85 -0.3 77,112 + GFF720 0.88 -1.3 77,135 - GFF720 0.90 -0.4 77,262 + +0.1 77,263 - -0.2 77,263 - +0.1 77,520 - -0.2 77,520 - -0.2 77,524 - -0.1 77,584 + +1.1 77,595 - -0.8 77,632 - GFF721 0.13 +1.1 77,668 + GFF721 0.18 -0.0 77,741 - GFF721 0.27 +0.5 77,820 + GFF721 0.37 +0.1 77,860 + GFF721 0.41 -0.1 77,861 - GFF721 0.42 -0.3 77,931 - GFF721 0.50 +0.0 77,964 + GFF721 0.54 -0.4 78,023 + GFF721 0.62 -0.1 78,024 - GFF721 0.62 -0.1 78,096 + GFF721 0.71 +0.2 78,205 + GFF721 0.84 -0.5
Or see this region's nucleotide sequence