Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF720

Experiment: LB_plus_SM_buffer with Ffm_phage 1.875 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF718 and GFF719 are separated by 28 nucleotidesGFF719 and GFF720 are separated by 393 nucleotidesGFF720 and GFF721 are separated by 261 nucleotides GFF718 - putative inner membrane protein, at 74,148 to 75,044 GFF718 GFF719 - HMP-PP hydrolase (pyridoxal phosphatase) Cof, detected in genetic screen for thiamin metabolic genes (PMID:15292217), at 75,073 to 75,603 GFF719 GFF720 - FIG01046262: hypothetical protein, at 75,997 to 77,262 GFF720 GFF721 - HMP-PP hydrolase (pyridoxal phosphatase) Cof, detected in genetic screen for thiamin metabolic genes (PMID:15292217), at 77,524 to 78,333 GFF721 Position (kb) 75 76 77 78Strain fitness (log2 ratio) -1 0 1 2at 75.138 kb on + strand, within GFF719at 75.139 kb on - strand, within GFF719at 75.373 kb on - strand, within GFF719at 75.552 kb on - strandat 75.615 kb on + strandat 75.744 kb on - strandat 75.746 kb on + strandat 75.746 kb on + strandat 75.846 kb on + strandat 75.911 kb on + strandat 76.007 kb on - strandat 76.007 kb on - strandat 76.012 kb on + strandat 76.076 kb on + strandat 76.181 kb on + strand, within GFF720at 76.181 kb on + strand, within GFF720at 76.403 kb on + strand, within GFF720at 76.588 kb on - strand, within GFF720at 76.716 kb on + strand, within GFF720at 76.768 kb on + strand, within GFF720at 76.907 kb on + strand, within GFF720at 76.907 kb on + strand, within GFF720at 76.907 kb on + strand, within GFF720at 76.907 kb on + strand, within GFF720at 77.073 kb on + strand, within GFF720at 77.074 kb on - strand, within GFF720at 77.112 kb on + strand, within GFF720at 77.135 kb on - strand, within GFF720at 77.262 kb on + strandat 77.263 kb on - strandat 77.263 kb on - strandat 77.520 kb on - strandat 77.520 kb on - strandat 77.524 kb on - strandat 77.584 kb on + strandat 77.595 kb on - strandat 77.632 kb on - strand, within GFF721at 77.668 kb on + strand, within GFF721at 77.741 kb on - strand, within GFF721at 77.820 kb on + strand, within GFF721at 77.860 kb on + strand, within GFF721at 77.861 kb on - strand, within GFF721at 77.931 kb on - strand, within GFF721at 77.964 kb on + strand, within GFF721at 78.023 kb on + strand, within GFF721at 78.024 kb on - strand, within GFF721at 78.096 kb on + strand, within GFF721at 78.205 kb on + strand, within GFF721

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 1.875 MOI
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75,138 + GFF719 0.12 +0.1
75,139 - GFF719 0.12 +0.3
75,373 - GFF719 0.56 +0.3
75,552 - +0.2
75,615 + +0.4
75,744 - +1.8
75,746 + -0.7
75,746 + -0.2
75,846 + -0.2
75,911 + -0.7
76,007 - -1.3
76,007 - -0.8
76,012 + -0.1
76,076 + -0.2
76,181 + GFF720 0.15 -0.5
76,181 + GFF720 0.15 -0.1
76,403 + GFF720 0.32 +0.1
76,588 - GFF720 0.47 +0.5
76,716 + GFF720 0.57 -0.3
76,768 + GFF720 0.61 -0.1
76,907 + GFF720 0.72 -0.2
76,907 + GFF720 0.72 +0.0
76,907 + GFF720 0.72 -0.4
76,907 + GFF720 0.72 -0.6
77,073 + GFF720 0.85 +0.6
77,074 - GFF720 0.85 -0.3
77,112 + GFF720 0.88 -1.3
77,135 - GFF720 0.90 -0.4
77,262 + +0.1
77,263 - -0.2
77,263 - +0.1
77,520 - -0.2
77,520 - -0.2
77,524 - -0.1
77,584 + +1.1
77,595 - -0.8
77,632 - GFF721 0.13 +1.1
77,668 + GFF721 0.18 -0.0
77,741 - GFF721 0.27 +0.5
77,820 + GFF721 0.37 +0.1
77,860 + GFF721 0.41 -0.1
77,861 - GFF721 0.42 -0.3
77,931 - GFF721 0.50 +0.0
77,964 + GFF721 0.54 -0.4
78,023 + GFF721 0.62 -0.1
78,024 - GFF721 0.62 -0.1
78,096 + GFF721 0.71 +0.2
78,205 + GFF721 0.84 -0.5

Or see this region's nucleotide sequence