Experiment: LB_plus_SM_buffer with Ffm_phage 1.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF580 and GFF581 are separated by 43 nucleotides GFF581 and GFF582 are separated by 43 nucleotides GFF582 and GFF583 overlap by 4 nucleotides
GFF580 - Transcriptional regulator, ArsR family, at 314,344 to 315,099
GFF580
GFF581 - 4-hydroxy-tetrahydrodipicolinate synthase (EC 4.3.3.7), at 315,143 to 316,048
GFF581
GFF582 - Dihydroxy-acid dehydratase (EC 4.2.1.9), at 316,092 to 317,807
GFF582
GFF583 - Nitrate/nitrite transporter, at 317,804 to 319,138
GFF583
Position (kb) 316
317
318 Strain fitness (log2 ratio) -1
0
1 at 315.162 kb on - strand at 315.218 kb on + strand at 315.219 kb on - strand at 315.294 kb on + strand, within GFF581 at 315.299 kb on - strand, within GFF581 at 315.369 kb on + strand, within GFF581 at 315.384 kb on - strand, within GFF581 at 315.432 kb on - strand, within GFF581 at 315.436 kb on + strand, within GFF581 at 315.436 kb on + strand, within GFF581 at 315.451 kb on + strand, within GFF581 at 315.452 kb on - strand, within GFF581 at 315.462 kb on + strand, within GFF581 at 315.506 kb on - strand, within GFF581 at 315.535 kb on + strand, within GFF581 at 315.590 kb on - strand, within GFF581 at 315.657 kb on - strand, within GFF581 at 315.691 kb on + strand, within GFF581 at 315.802 kb on + strand, within GFF581 at 315.802 kb on + strand, within GFF581 at 315.805 kb on + strand, within GFF581 at 315.806 kb on - strand, within GFF581 at 315.809 kb on + strand, within GFF581 at 315.813 kb on - strand, within GFF581 at 315.813 kb on - strand, within GFF581 at 315.824 kb on - strand, within GFF581 at 315.835 kb on + strand, within GFF581 at 315.915 kb on - strand, within GFF581 at 316.020 kb on - strand at 316.050 kb on + strand at 316.051 kb on - strand at 316.093 kb on + strand at 316.094 kb on - strand at 316.098 kb on - strand at 316.117 kb on - strand at 316.251 kb on + strand at 316.434 kb on + strand, within GFF582 at 316.583 kb on + strand, within GFF582 at 316.620 kb on - strand, within GFF582 at 316.656 kb on + strand, within GFF582 at 316.694 kb on + strand, within GFF582 at 316.752 kb on - strand, within GFF582 at 316.894 kb on + strand, within GFF582 at 316.894 kb on + strand, within GFF582 at 317.030 kb on + strand, within GFF582 at 317.375 kb on + strand, within GFF582 at 317.375 kb on + strand, within GFF582 at 317.390 kb on + strand, within GFF582 at 317.391 kb on - strand, within GFF582 at 317.391 kb on - strand, within GFF582 at 317.479 kb on + strand, within GFF582 at 317.542 kb on + strand, within GFF582 at 317.543 kb on - strand, within GFF582 at 317.543 kb on - strand, within GFF582 at 317.689 kb on + strand at 317.897 kb on + strand at 317.898 kb on - strand at 317.898 kb on - strand at 318.074 kb on + strand, within GFF583 at 318.131 kb on - strand, within GFF583 at 318.170 kb on + strand, within GFF583 at 318.171 kb on - strand, within GFF583 at 318.244 kb on + strand, within GFF583 at 318.264 kb on + strand, within GFF583 at 318.264 kb on + strand, within GFF583 at 318.264 kb on + strand, within GFF583 at 318.265 kb on - strand, within GFF583 at 318.378 kb on + strand, within GFF583 at 318.379 kb on - strand, within GFF583 at 318.403 kb on - strand, within GFF583 at 318.403 kb on - strand, within GFF583 at 318.614 kb on - strand, within GFF583 at 318.634 kb on - strand, within GFF583 at 318.643 kb on + strand, within GFF583 at 318.673 kb on + strand, within GFF583 at 318.674 kb on - strand, within GFF583 at 318.768 kb on + strand, within GFF583
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 1.875 MOI remove 315,162 - +0.1 315,218 + +0.2 315,219 - -0.1 315,294 + GFF581 0.17 +0.3 315,299 - GFF581 0.17 +0.0 315,369 + GFF581 0.25 -0.3 315,384 - GFF581 0.27 -0.6 315,432 - GFF581 0.32 +0.1 315,436 + GFF581 0.32 +0.3 315,436 + GFF581 0.32 +0.3 315,451 + GFF581 0.34 -0.0 315,452 - GFF581 0.34 -0.2 315,462 + GFF581 0.35 +0.0 315,506 - GFF581 0.40 +0.6 315,535 + GFF581 0.43 -0.9 315,590 - GFF581 0.49 +0.1 315,657 - GFF581 0.57 -0.2 315,691 + GFF581 0.60 +0.2 315,802 + GFF581 0.73 -0.3 315,802 + GFF581 0.73 -0.2 315,805 + GFF581 0.73 +0.2 315,806 - GFF581 0.73 -0.6 315,809 + GFF581 0.74 -0.4 315,813 - GFF581 0.74 +0.2 315,813 - GFF581 0.74 +0.1 315,824 - GFF581 0.75 -0.0 315,835 + GFF581 0.76 -0.8 315,915 - GFF581 0.85 -0.5 316,020 - +0.1 316,050 + -0.7 316,051 - -0.0 316,093 + -0.2 316,094 - -0.4 316,098 - +0.0 316,117 - +0.5 316,251 + +0.1 316,434 + GFF582 0.20 +0.1 316,583 + GFF582 0.29 -0.8 316,620 - GFF582 0.31 +0.0 316,656 + GFF582 0.33 +0.0 316,694 + GFF582 0.35 +1.0 316,752 - GFF582 0.38 +0.1 316,894 + GFF582 0.47 +0.1 316,894 + GFF582 0.47 -0.0 317,030 + GFF582 0.55 +0.9 317,375 + GFF582 0.75 -0.3 317,375 + GFF582 0.75 +0.3 317,390 + GFF582 0.76 +1.0 317,391 - GFF582 0.76 +0.1 317,391 - GFF582 0.76 +0.4 317,479 + GFF582 0.81 +0.2 317,542 + GFF582 0.84 +0.7 317,543 - GFF582 0.85 +0.2 317,543 - GFF582 0.85 +0.1 317,689 + -0.5 317,897 + -0.1 317,898 - +1.1 317,898 - -0.0 318,074 + GFF583 0.20 -0.3 318,131 - GFF583 0.24 +0.2 318,170 + GFF583 0.27 +0.6 318,171 - GFF583 0.27 -0.1 318,244 + GFF583 0.33 +0.2 318,264 + GFF583 0.34 +0.9 318,264 + GFF583 0.34 -0.2 318,264 + GFF583 0.34 -0.9 318,265 - GFF583 0.35 -0.1 318,378 + GFF583 0.43 -0.5 318,379 - GFF583 0.43 -0.2 318,403 - GFF583 0.45 -1.4 318,403 - GFF583 0.45 +0.8 318,614 - GFF583 0.61 +0.0 318,634 - GFF583 0.62 -0.2 318,643 + GFF583 0.63 +0.6 318,673 + GFF583 0.65 +0.0 318,674 - GFF583 0.65 +0.1 318,768 + GFF583 0.72 +0.5
Or see this region's nucleotide sequence