Experiment: LB_plus_SM_buffer with Ffm_phage 1.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF511 and GFF512 are separated by 58 nucleotides GFF512 and GFF513 are separated by 46 nucleotides
GFF511 - anaerobic C4-dicarboxylate transporter, at 240,665 to 241,984
GFF511
GFF512 - L-asparaginase (EC 3.5.1.1), at 242,043 to 243,086
GFF512
GFF513 - Glutathione reductase (EC 1.8.1.7), at 243,133 to 244,485
GFF513
Position (kb) 242
243
244 Strain fitness (log2 ratio) -1
0
1 at 241.057 kb on - strand, within GFF511 at 241.103 kb on - strand, within GFF511 at 241.169 kb on - strand, within GFF511 at 241.229 kb on - strand, within GFF511 at 241.288 kb on - strand, within GFF511 at 241.507 kb on - strand, within GFF511 at 241.684 kb on + strand, within GFF511 at 241.685 kb on - strand, within GFF511 at 241.782 kb on + strand, within GFF511 at 241.880 kb on - strand at 241.889 kb on + strand at 242.096 kb on - strand at 242.169 kb on - strand, within GFF512 at 242.376 kb on - strand, within GFF512 at 242.410 kb on - strand, within GFF512 at 242.418 kb on - strand, within GFF512 at 242.609 kb on + strand, within GFF512 at 242.653 kb on - strand, within GFF512 at 242.734 kb on + strand, within GFF512 at 242.739 kb on - strand, within GFF512 at 242.739 kb on - strand, within GFF512 at 242.798 kb on + strand, within GFF512 at 242.798 kb on + strand, within GFF512 at 242.802 kb on + strand, within GFF512 at 242.802 kb on + strand, within GFF512 at 242.802 kb on + strand, within GFF512 at 242.802 kb on + strand, within GFF512 at 242.803 kb on - strand, within GFF512 at 242.838 kb on - strand, within GFF512 at 242.915 kb on + strand, within GFF512 at 242.954 kb on - strand, within GFF512 at 242.964 kb on - strand, within GFF512 at 243.078 kb on - strand at 243.081 kb on + strand at 243.085 kb on - strand at 243.132 kb on - strand at 243.134 kb on + strand at 243.349 kb on - strand, within GFF513 at 243.350 kb on + strand, within GFF513 at 243.350 kb on + strand, within GFF513 at 243.350 kb on + strand, within GFF513 at 243.350 kb on + strand, within GFF513 at 243.351 kb on - strand, within GFF513 at 243.354 kb on + strand, within GFF513 at 243.373 kb on - strand, within GFF513 at 243.373 kb on - strand, within GFF513 at 243.480 kb on - strand, within GFF513 at 243.507 kb on + strand, within GFF513 at 243.557 kb on - strand, within GFF513 at 243.563 kb on - strand, within GFF513 at 243.635 kb on + strand, within GFF513 at 243.675 kb on + strand, within GFF513 at 243.675 kb on + strand, within GFF513 at 243.678 kb on + strand, within GFF513 at 243.956 kb on + strand, within GFF513 at 243.998 kb on - strand, within GFF513 at 244.028 kb on + strand, within GFF513
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 1.875 MOI remove 241,057 - GFF511 0.30 +0.0 241,103 - GFF511 0.33 -0.6 241,169 - GFF511 0.38 +0.3 241,229 - GFF511 0.43 -0.1 241,288 - GFF511 0.47 +0.6 241,507 - GFF511 0.64 +0.2 241,684 + GFF511 0.77 +0.2 241,685 - GFF511 0.77 -0.1 241,782 + GFF511 0.85 +0.6 241,880 - -0.4 241,889 + -0.0 242,096 - +0.1 242,169 - GFF512 0.12 +0.1 242,376 - GFF512 0.32 -0.2 242,410 - GFF512 0.35 +0.6 242,418 - GFF512 0.36 +1.1 242,609 + GFF512 0.54 +0.8 242,653 - GFF512 0.58 +0.7 242,734 + GFF512 0.66 +0.2 242,739 - GFF512 0.67 +0.8 242,739 - GFF512 0.67 -0.2 242,798 + GFF512 0.72 +0.1 242,798 + GFF512 0.72 +0.0 242,802 + GFF512 0.73 +1.2 242,802 + GFF512 0.73 +0.6 242,802 + GFF512 0.73 +0.6 242,802 + GFF512 0.73 -0.0 242,803 - GFF512 0.73 -0.1 242,838 - GFF512 0.76 +0.3 242,915 + GFF512 0.84 +0.4 242,954 - GFF512 0.87 +0.0 242,964 - GFF512 0.88 +0.3 243,078 - -0.2 243,081 + +0.9 243,085 - -0.2 243,132 - +0.1 243,134 + +0.2 243,349 - GFF513 0.16 +0.3 243,350 + GFF513 0.16 -0.0 243,350 + GFF513 0.16 -0.3 243,350 + GFF513 0.16 +0.1 243,350 + GFF513 0.16 +0.8 243,351 - GFF513 0.16 +0.1 243,354 + GFF513 0.16 +0.1 243,373 - GFF513 0.18 +1.2 243,373 - GFF513 0.18 +0.0 243,480 - GFF513 0.26 +0.4 243,507 + GFF513 0.28 +0.7 243,557 - GFF513 0.31 +0.4 243,563 - GFF513 0.32 -0.2 243,635 + GFF513 0.37 +0.7 243,675 + GFF513 0.40 +0.3 243,675 + GFF513 0.40 +0.3 243,678 + GFF513 0.40 -0.5 243,956 + GFF513 0.61 +0.3 243,998 - GFF513 0.64 +0.1 244,028 + GFF513 0.66 -0.3
Or see this region's nucleotide sequence