Experiment: LB_plus_SM_buffer with Ffm_phage 1.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4872 and GFF4873 are separated by 62 nucleotides GFF4873 and GFF4874 are separated by 3 nucleotides GFF4874 and GFF4690 are separated by 303 nucleotides GFF4690 and GFF4691 are separated by 234 nucleotides GFF4691 and GFF4692 are separated by 235 nucleotides GFF4692 and GFF4693 are separated by 116 nucleotides
GFF4872 - tRNA-Arg-ACG, at 116 to 192
GFF4872
GFF4873 - tRNA-Arg-ACG, at 255 to 331
GFF4873
GFF4874 - tRNA-Ser-GCT, at 335 to 427
GFF4874
GFF4690 - Carbon storage regulator, at 731 to 916
GFF4690
GFF4691 - Alanyl-tRNA synthetase (EC 6.1.1.7), at 1,151 to 3,781
GFF4691
GFF4692 - Regulatory protein RecX, at 4,017 to 4,517
GFF4692
GFF4693 - RecA protein, at 4,634 to 5,695
GFF4693
Position (kb) 1
2
3
4 Strain fitness (log2 ratio) -1
0
1 at 0.249 kb on - strand at 0.439 kb on - strand at 0.561 kb on + strand at 0.561 kb on + strand at 0.732 kb on + strand at 0.733 kb on - strand at 0.977 kb on + strand at 0.977 kb on + strand at 0.977 kb on + strand at 1.011 kb on + strand at 1.012 kb on - strand at 1.033 kb on + strand at 1.033 kb on + strand at 1.085 kb on + strand at 1.149 kb on - strand at 3.904 kb on - strand at 4.039 kb on - strand at 4.039 kb on - strand at 4.132 kb on + strand, within GFF4692 at 4.315 kb on + strand, within GFF4692 at 4.316 kb on - strand, within GFF4692 at 4.316 kb on - strand, within GFF4692 at 4.457 kb on + strand, within GFF4692 at 4.457 kb on + strand, within GFF4692 at 4.458 kb on - strand, within GFF4692 at 4.461 kb on + strand, within GFF4692 at 4.556 kb on + strand at 4.557 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 1.875 MOI remove 249 - -0.1 439 - -1.1 561 + +0.0 561 + -0.1 732 + +0.4 733 - -0.1 977 + -0.5 977 + -0.3 977 + -0.9 1,011 + +0.7 1,012 - +0.1 1,033 + -0.5 1,033 + -0.4 1,085 + -0.2 1,149 - -0.3 3,904 - -1.4 4,039 - +0.1 4,039 - +0.6 4,132 + GFF4692 0.23 -0.7 4,315 + GFF4692 0.59 -0.2 4,316 - GFF4692 0.60 -0.0 4,316 - GFF4692 0.60 -0.8 4,457 + GFF4692 0.88 +1.2 4,457 + GFF4692 0.88 +0.2 4,458 - GFF4692 0.88 -0.2 4,461 + GFF4692 0.89 +0.1 4,556 + +1.2 4,557 - -0.2
Or see this region's nucleotide sequence