Experiment: LB_plus_SM_buffer with Ffm_phage 1.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4678 and GFF4679 are separated by 11 nucleotides GFF4679 and GFF4680 are separated by 45 nucleotides GFF4680 and GFF4681 are separated by 62 nucleotides GFF4681 and GFF4682 overlap by 20 nucleotides
GFF4678 - Phage minor tail protein, at 8,311 to 8,712
GFF4678
GFF4679 - Phage tail assembly, at 8,724 to 9,473
GFF4679
GFF4680 - Phage minor tail protein, at 9,519 to 9,917
GFF4680
GFF4681 - Phage minor tail protein, at 9,980 to 10,243
GFF4681
GFF4682 - Phage tail length tape-measure protein 1, at 10,224 to 13,310
GFF4682
Position (kb)
9
10 Strain fitness (log2 ratio)
-1
0
1
2 at 8.637 kb on + strand, within GFF4678 at 8.637 kb on + strand, within GFF4678 at 8.637 kb on + strand, within GFF4678 at 8.638 kb on - strand, within GFF4678 at 8.638 kb on - strand, within GFF4678 at 8.641 kb on + strand, within GFF4678 at 8.701 kb on + strand at 8.701 kb on + strand at 8.702 kb on - strand at 8.791 kb on - strand at 8.791 kb on - strand at 8.834 kb on + strand, within GFF4679 at 8.901 kb on - strand, within GFF4679 at 8.904 kb on - strand, within GFF4679 at 8.904 kb on - strand, within GFF4679 at 9.180 kb on + strand, within GFF4679 at 9.181 kb on - strand, within GFF4679 at 9.213 kb on - strand, within GFF4679 at 9.382 kb on + strand, within GFF4679 at 9.392 kb on + strand, within GFF4679 at 9.514 kb on - strand at 9.514 kb on - strand at 9.593 kb on + strand, within GFF4680 at 9.594 kb on - strand, within GFF4680 at 9.623 kb on + strand, within GFF4680 at 9.634 kb on - strand, within GFF4680 at 9.681 kb on + strand, within GFF4680 at 9.681 kb on + strand, within GFF4680 at 9.682 kb on - strand, within GFF4680 at 9.682 kb on - strand, within GFF4680 at 10.268 kb on + strand at 10.460 kb on + strand at 10.610 kb on - strand, within GFF4682 at 10.610 kb on - strand, within GFF4682
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 1.875 MOI remove 8,637 + GFF4678 0.81 -0.1 8,637 + GFF4678 0.81 +0.1 8,637 + GFF4678 0.81 -0.1 8,638 - GFF4678 0.81 -1.0 8,638 - GFF4678 0.81 +0.2 8,641 + GFF4678 0.82 +0.8 8,701 + +0.1 8,701 + -0.2 8,702 - +0.2 8,791 - +0.1 8,791 - +1.3 8,834 + GFF4679 0.15 +0.4 8,901 - GFF4679 0.24 -0.1 8,904 - GFF4679 0.24 +1.4 8,904 - GFF4679 0.24 +0.7 9,180 + GFF4679 0.61 -0.0 9,181 - GFF4679 0.61 -0.2 9,213 - GFF4679 0.65 +0.4 9,382 + GFF4679 0.88 +0.0 9,392 + GFF4679 0.89 -0.1 9,514 - -0.0 9,514 - +0.1 9,593 + GFF4680 0.19 +0.1 9,594 - GFF4680 0.19 -0.8 9,623 + GFF4680 0.26 +1.1 9,634 - GFF4680 0.29 +0.3 9,681 + GFF4680 0.41 -0.1 9,681 + GFF4680 0.41 +0.2 9,682 - GFF4680 0.41 +0.2 9,682 - GFF4680 0.41 +1.9 10,268 + +0.4 10,460 + +0.4 10,610 - GFF4682 0.13 -0.1 10,610 - GFF4682 0.13 +1.3
Or see this region's nucleotide sequence