Experiment: LB_plus_SM_buffer with Ffm_phage 1.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4674 and GFF4675 are separated by 90 nucleotides GFF4675 and GFF4676 overlap by 8 nucleotides GFF4676 and GFF4677 overlap by 20 nucleotides GFF4677 and GFF4678 overlap by 4 nucleotides
GFF4674 - Prophage Clp protease-like protein, at 5,007 to 7,061
GFF4674
GFF4675 - putative RecA/RadA recombinase, at 7,152 to 7,475
GFF4675
GFF4676 - ATP-binding sugar transporter-like protein, at 7,468 to 7,767
GFF4676
GFF4677 - Phage tail completion protein, at 7,748 to 8,314
GFF4677
GFF4678 - Phage minor tail protein, at 8,311 to 8,712
GFF4678
Position (kb) 7
8 Strain fitness (log2 ratio) -1
0
1 at 6.186 kb on - strand, within GFF4674 at 6.384 kb on + strand, within GFF4674 at 6.384 kb on + strand, within GFF4674 at 6.476 kb on + strand, within GFF4674 at 6.483 kb on - strand, within GFF4674 at 6.483 kb on - strand, within GFF4674 at 6.674 kb on - strand, within GFF4674 at 6.771 kb on - strand, within GFF4674 at 6.891 kb on - strand at 6.951 kb on + strand at 6.953 kb on + strand at 7.058 kb on - strand at 7.058 kb on - strand at 7.210 kb on + strand, within GFF4675 at 7.266 kb on - strand, within GFF4675 at 7.307 kb on - strand, within GFF4675 at 7.408 kb on - strand, within GFF4675 at 7.408 kb on - strand, within GFF4675 at 7.562 kb on + strand, within GFF4676 at 7.622 kb on + strand, within GFF4676 at 7.623 kb on - strand, within GFF4676 at 7.689 kb on + strand, within GFF4676 at 7.755 kb on + strand at 7.756 kb on - strand at 7.805 kb on + strand, within GFF4677 at 7.991 kb on + strand, within GFF4677 at 7.992 kb on - strand, within GFF4677 at 7.992 kb on - strand, within GFF4677 at 8.068 kb on + strand, within GFF4677 at 8.069 kb on - strand, within GFF4677 at 8.069 kb on - strand, within GFF4677 at 8.076 kb on + strand, within GFF4677 at 8.082 kb on + strand, within GFF4677 at 8.176 kb on + strand, within GFF4677 at 8.441 kb on - strand, within GFF4678 at 8.458 kb on + strand, within GFF4678
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 1.875 MOI remove 6,186 - GFF4674 0.57 -0.3 6,384 + GFF4674 0.67 +0.1 6,384 + GFF4674 0.67 +0.0 6,476 + GFF4674 0.71 +0.2 6,483 - GFF4674 0.72 -0.1 6,483 - GFF4674 0.72 -0.5 6,674 - GFF4674 0.81 -0.1 6,771 - GFF4674 0.86 -0.5 6,891 - +1.3 6,951 + +0.0 6,953 + +0.6 7,058 - +0.3 7,058 - +0.1 7,210 + GFF4675 0.18 -0.6 7,266 - GFF4675 0.35 -0.4 7,307 - GFF4675 0.48 -1.0 7,408 - GFF4675 0.79 -1.0 7,408 - GFF4675 0.79 -0.2 7,562 + GFF4676 0.31 -0.2 7,622 + GFF4676 0.51 -0.3 7,623 - GFF4676 0.52 -0.5 7,689 + GFF4676 0.74 +0.1 7,755 + +0.4 7,756 - -1.1 7,805 + GFF4677 0.10 -0.8 7,991 + GFF4677 0.43 -0.6 7,992 - GFF4677 0.43 -0.2 7,992 - GFF4677 0.43 +0.8 8,068 + GFF4677 0.56 +0.3 8,069 - GFF4677 0.57 -0.4 8,069 - GFF4677 0.57 +0.2 8,076 + GFF4677 0.58 +0.1 8,082 + GFF4677 0.59 -0.2 8,176 + GFF4677 0.75 +0.4 8,441 - GFF4678 0.32 -0.3 8,458 + GFF4678 0.37 -0.8
Or see this region's nucleotide sequence