Experiment: LB_plus_SM_buffer with Ffm_phage 1.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4616 and GFF4617 overlap by 7 nucleotides GFF4617 and GFF4618 overlap by 11 nucleotides GFF4618 and GFF4619 are separated by 323 nucleotides GFF4619 and GFF4620 are separated by 59 nucleotides
GFF4616 - Allophanate hydrolase 2 subunit 1 (EC 3.5.1.54), at 409,351 to 410,007
GFF4616
GFF4617 - Allophanate hydrolase 2 subunit 2 (EC 3.5.1.54), at 410,001 to 410,933
GFF4617
GFF4618 - Lactam utilization protein LamB, at 410,923 to 411,657
GFF4618
GFF4619 - hypothetical protein, at 411,981 to 412,112
GFF4619
GFF4620 - Probable DNA recombinase, at 412,172 to 412,738
GFF4620
Position (kb) 410
411
412 Strain fitness (log2 ratio) -1
0
1 at 410.009 kb on + strand at 410.010 kb on - strand at 410.012 kb on + strand at 410.030 kb on + strand at 410.300 kb on + strand, within GFF4617 at 410.330 kb on + strand, within GFF4617 at 410.331 kb on - strand, within GFF4617 at 410.331 kb on - strand, within GFF4617 at 410.383 kb on - strand, within GFF4617 at 410.383 kb on - strand, within GFF4617 at 410.384 kb on + strand, within GFF4617 at 410.384 kb on + strand, within GFF4617 at 410.504 kb on - strand, within GFF4617 at 410.504 kb on - strand, within GFF4617 at 410.713 kb on - strand, within GFF4617 at 410.726 kb on + strand, within GFF4617 at 410.727 kb on - strand, within GFF4617 at 410.795 kb on + strand, within GFF4617 at 410.795 kb on + strand, within GFF4617 at 410.795 kb on + strand, within GFF4617 at 410.796 kb on - strand, within GFF4617 at 410.796 kb on - strand, within GFF4617 at 410.906 kb on + strand at 411.158 kb on - strand, within GFF4618 at 411.250 kb on + strand, within GFF4618 at 411.251 kb on - strand, within GFF4618 at 411.310 kb on + strand, within GFF4618 at 411.311 kb on - strand, within GFF4618 at 411.429 kb on + strand, within GFF4618 at 411.506 kb on + strand, within GFF4618 at 411.506 kb on + strand, within GFF4618 at 411.506 kb on + strand, within GFF4618 at 411.507 kb on - strand, within GFF4618 at 411.507 kb on - strand, within GFF4618 at 411.681 kb on + strand at 411.821 kb on - strand at 411.859 kb on + strand at 412.063 kb on - strand, within GFF4619 at 412.088 kb on + strand, within GFF4619 at 412.350 kb on - strand, within GFF4620 at 412.350 kb on - strand, within GFF4620 at 412.406 kb on + strand, within GFF4620 at 412.406 kb on + strand, within GFF4620 at 412.409 kb on - strand, within GFF4620 at 412.443 kb on - strand, within GFF4620 at 412.577 kb on - strand, within GFF4620 at 412.577 kb on - strand, within GFF4620 at 412.625 kb on - strand, within GFF4620 at 412.635 kb on + strand, within GFF4620 at 412.636 kb on - strand, within GFF4620 at 412.636 kb on - strand, within GFF4620 at 412.643 kb on + strand, within GFF4620
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 1.875 MOI remove 410,009 + +0.6 410,010 - +0.7 410,012 + +0.5 410,030 + +0.5 410,300 + GFF4617 0.32 +0.5 410,330 + GFF4617 0.35 +0.3 410,331 - GFF4617 0.35 +0.8 410,331 - GFF4617 0.35 -0.2 410,383 - GFF4617 0.41 +0.2 410,383 - GFF4617 0.41 +0.1 410,384 + GFF4617 0.41 +0.2 410,384 + GFF4617 0.41 -0.2 410,504 - GFF4617 0.54 +0.3 410,504 - GFF4617 0.54 +0.5 410,713 - GFF4617 0.76 +0.2 410,726 + GFF4617 0.78 +0.5 410,727 - GFF4617 0.78 +0.1 410,795 + GFF4617 0.85 -0.6 410,795 + GFF4617 0.85 +0.1 410,795 + GFF4617 0.85 -0.0 410,796 - GFF4617 0.85 +0.4 410,796 - GFF4617 0.85 +0.3 410,906 + +0.2 411,158 - GFF4618 0.32 +0.9 411,250 + GFF4618 0.44 +0.3 411,251 - GFF4618 0.45 +0.5 411,310 + GFF4618 0.53 -0.0 411,311 - GFF4618 0.53 +0.3 411,429 + GFF4618 0.69 +0.1 411,506 + GFF4618 0.79 +0.1 411,506 + GFF4618 0.79 +0.6 411,506 + GFF4618 0.79 -0.9 411,507 - GFF4618 0.79 +1.1 411,507 - GFF4618 0.79 +0.1 411,681 + +0.2 411,821 - +0.4 411,859 + -0.1 412,063 - GFF4619 0.62 +0.4 412,088 + GFF4619 0.81 +0.6 412,350 - GFF4620 0.31 -0.4 412,350 - GFF4620 0.31 +0.2 412,406 + GFF4620 0.41 -0.0 412,406 + GFF4620 0.41 +0.6 412,409 - GFF4620 0.42 +0.3 412,443 - GFF4620 0.48 -0.1 412,577 - GFF4620 0.71 +0.3 412,577 - GFF4620 0.71 +0.1 412,625 - GFF4620 0.80 +0.2 412,635 + GFF4620 0.82 +0.3 412,636 - GFF4620 0.82 -0.3 412,636 - GFF4620 0.82 -0.2 412,643 + GFF4620 0.83 +0.4
Or see this region's nucleotide sequence