Experiment: LB_plus_SM_buffer with Ffm_phage 1.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4530 and GFF4531 are separated by 210 nucleotides GFF4531 and GFF4532 are separated by 9 nucleotides GFF4532 and GFF4533 overlap by 32 nucleotides GFF4533 and GFF4534 are separated by 53 nucleotides
GFF4530 - [Citrate [pro-3S]-lyase] ligase (EC 6.2.1.22), at 316,780 to 317,856
GFF4530
GFF4531 - FIG01046674: hypothetical protein, at 318,067 to 318,231
GFF4531
GFF4532 - Sensor kinase CitA, DpiB (EC 2.7.3.-), at 318,241 to 319,902
GFF4532
GFF4533 - Transcriptional regulatory protein CitB, DpiA, at 319,871 to 320,551
GFF4533
GFF4534 - C4-dicarboxylate transporter DcuC (TC 2.A.61.1.1), at 320,605 to 321,948
GFF4534
Position (kb) 318
319
320 Strain fitness (log2 ratio) -1
0
1 at 317.307 kb on + strand, within GFF4530 at 317.308 kb on - strand, within GFF4530 at 317.409 kb on + strand, within GFF4530 at 317.410 kb on - strand, within GFF4530 at 317.410 kb on - strand, within GFF4530 at 317.410 kb on - strand, within GFF4530 at 317.492 kb on - strand, within GFF4530 at 317.556 kb on - strand, within GFF4530 at 318.358 kb on + strand at 318.529 kb on + strand, within GFF4532 at 318.641 kb on - strand, within GFF4532 at 318.694 kb on - strand, within GFF4532 at 318.751 kb on - strand, within GFF4532 at 318.822 kb on - strand, within GFF4532 at 318.925 kb on - strand, within GFF4532 at 318.970 kb on - strand, within GFF4532 at 319.274 kb on + strand, within GFF4532 at 319.333 kb on + strand, within GFF4532 at 319.333 kb on + strand, within GFF4532 at 319.387 kb on - strand, within GFF4532 at 319.392 kb on + strand, within GFF4532 at 319.502 kb on + strand, within GFF4532 at 319.502 kb on + strand, within GFF4532 at 319.502 kb on + strand, within GFF4532 at 319.649 kb on + strand, within GFF4532 at 319.718 kb on + strand, within GFF4532 at 319.718 kb on + strand, within GFF4532 at 319.719 kb on - strand, within GFF4532 at 319.719 kb on - strand, within GFF4532 at 319.719 kb on - strand, within GFF4532 at 319.719 kb on - strand, within GFF4532 at 319.719 kb on - strand, within GFF4532 at 319.891 kb on - strand at 319.938 kb on - strand at 320.029 kb on - strand, within GFF4533 at 320.041 kb on + strand, within GFF4533 at 320.045 kb on + strand, within GFF4533 at 320.195 kb on - strand, within GFF4533 at 320.286 kb on + strand, within GFF4533 at 320.286 kb on + strand, within GFF4533 at 320.503 kb on + strand at 320.533 kb on + strand at 320.533 kb on + strand at 320.816 kb on + strand, within GFF4534 at 320.850 kb on + strand, within GFF4534
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 1.875 MOI remove 317,307 + GFF4530 0.49 +0.4 317,308 - GFF4530 0.49 -0.1 317,409 + GFF4530 0.58 -0.2 317,410 - GFF4530 0.58 -0.1 317,410 - GFF4530 0.58 +1.0 317,410 - GFF4530 0.58 +0.0 317,492 - GFF4530 0.66 -0.1 317,556 - GFF4530 0.72 -0.2 318,358 + -0.3 318,529 + GFF4532 0.17 +0.5 318,641 - GFF4532 0.24 +0.3 318,694 - GFF4532 0.27 +0.2 318,751 - GFF4532 0.31 +0.3 318,822 - GFF4532 0.35 +0.2 318,925 - GFF4532 0.41 +0.4 318,970 - GFF4532 0.44 +0.0 319,274 + GFF4532 0.62 +0.5 319,333 + GFF4532 0.66 -0.1 319,333 + GFF4532 0.66 -0.1 319,387 - GFF4532 0.69 +0.2 319,392 + GFF4532 0.69 -0.0 319,502 + GFF4532 0.76 -0.2 319,502 + GFF4532 0.76 +0.3 319,502 + GFF4532 0.76 -0.0 319,649 + GFF4532 0.85 +0.9 319,718 + GFF4532 0.89 +0.4 319,718 + GFF4532 0.89 +0.2 319,719 - GFF4532 0.89 +0.4 319,719 - GFF4532 0.89 -0.1 319,719 - GFF4532 0.89 +0.1 319,719 - GFF4532 0.89 +0.1 319,719 - GFF4532 0.89 -0.1 319,891 - -0.0 319,938 - +0.1 320,029 - GFF4533 0.23 +0.5 320,041 + GFF4533 0.25 -0.0 320,045 + GFF4533 0.26 -0.2 320,195 - GFF4533 0.48 +0.2 320,286 + GFF4533 0.61 +0.1 320,286 + GFF4533 0.61 +0.1 320,503 + -0.0 320,533 + +0.1 320,533 + +0.5 320,816 + GFF4534 0.16 -0.2 320,850 + GFF4534 0.18 +1.2
Or see this region's nucleotide sequence