Experiment: LB_plus_SM_buffer with Ffm_phage 1.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4354 and GFF4355 are separated by 185 nucleotides GFF4355 and GFF4356 are separated by 208 nucleotides GFF4356 and GFF4357 are separated by 290 nucleotides
GFF4354 - ATP-dependent Clp protease ATP-binding subunit ClpX, at 134,297 to 135,568
GFF4354
GFF4355 - ATP-dependent protease La (EC 3.4.21.53) Type I, at 135,754 to 138,108
GFF4355
GFF4356 - DNA-binding protein HU-beta, at 138,317 to 138,589
GFF4356
GFF4357 - Peptidyl-prolyl cis-trans isomerase PpiD (EC 5.2.1.8), at 138,880 to 140,751
GFF4357
Position (kb)
135
136
137
138
139 Strain fitness (log2 ratio)
-2
-1
0
1 at 135.010 kb on - strand, within GFF4354 at 135.013 kb on + strand, within GFF4354 at 135.449 kb on + strand at 135.449 kb on + strand at 135.671 kb on + strand at 135.679 kb on + strand at 135.680 kb on - strand at 135.689 kb on - strand at 135.708 kb on + strand at 135.708 kb on + strand at 135.708 kb on + strand at 135.709 kb on - strand at 135.709 kb on - strand at 135.710 kb on + strand at 136.946 kb on - strand, within GFF4355 at 137.440 kb on - strand, within GFF4355 at 137.947 kb on - strand at 138.179 kb on + strand at 138.186 kb on - strand at 138.390 kb on + strand, within GFF4356 at 138.401 kb on - strand, within GFF4356 at 138.582 kb on + strand at 138.587 kb on + strand at 138.620 kb on - strand at 138.763 kb on + strand at 138.848 kb on + strand at 139.000 kb on + strand at 139.068 kb on + strand, within GFF4357 at 139.074 kb on + strand, within GFF4357
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 1.875 MOI remove 135,010 - GFF4354 0.56 -1.3 135,013 + GFF4354 0.56 +0.2 135,449 + -0.9 135,449 + -1.4 135,671 + -0.1 135,679 + -0.5 135,680 - -0.2 135,689 - -0.1 135,708 + -0.6 135,708 + -0.4 135,708 + -0.3 135,709 - -2.1 135,709 - -0.7 135,710 + -0.5 136,946 - GFF4355 0.51 -0.6 137,440 - GFF4355 0.72 -1.3 137,947 - -2.6 138,179 + -0.4 138,186 - -0.2 138,390 + GFF4356 0.27 -0.0 138,401 - GFF4356 0.31 -0.2 138,582 + -0.2 138,587 + -0.1 138,620 - +0.3 138,763 + +0.3 138,848 + +0.3 139,000 + +0.3 139,068 + GFF4357 0.10 -1.2 139,074 + GFF4357 0.10 +0.6
Or see this region's nucleotide sequence