Experiment: LB_plus_SM_buffer with Ffm_phage 1.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF423 and GFF424 are separated by 117 nucleotides GFF424 and GFF425 are separated by 263 nucleotides
GFF423 - Aldehyde dehydrogenase B (EC 1.2.1.22), at 143,567 to 145,105
GFF423
GFF424 - Putative glycosyl hydrolase of unknown function (DUF1680), at 145,223 to 147,178
GFF424
GFF425 - Putative arylsulfatase regulatory protein, at 147,442 to 148,257
GFF425
Position (kb) 145
146
147
148 Strain fitness (log2 ratio) -1
0
1 at 144.336 kb on - strand, within GFF423 at 144.392 kb on - strand, within GFF423 at 144.392 kb on - strand, within GFF423 at 144.470 kb on - strand, within GFF423 at 144.487 kb on + strand, within GFF423 at 144.487 kb on + strand, within GFF423 at 144.488 kb on - strand, within GFF423 at 144.525 kb on - strand, within GFF423 at 144.595 kb on + strand, within GFF423 at 144.647 kb on + strand, within GFF423 at 144.648 kb on - strand, within GFF423 at 144.649 kb on + strand, within GFF423 at 144.721 kb on + strand, within GFF423 at 144.734 kb on + strand, within GFF423 at 144.746 kb on - strand, within GFF423 at 144.769 kb on + strand, within GFF423 at 144.824 kb on - strand, within GFF423 at 144.960 kb on - strand at 144.998 kb on - strand at 145.167 kb on - strand at 145.333 kb on - strand at 145.403 kb on - strand at 145.466 kb on - strand, within GFF424 at 145.567 kb on - strand, within GFF424 at 145.718 kb on + strand, within GFF424 at 145.785 kb on + strand, within GFF424 at 145.851 kb on + strand, within GFF424 at 145.852 kb on - strand, within GFF424 at 145.852 kb on - strand, within GFF424 at 145.852 kb on - strand, within GFF424 at 145.855 kb on + strand, within GFF424 at 145.856 kb on - strand, within GFF424 at 145.863 kb on + strand, within GFF424 at 145.864 kb on - strand, within GFF424 at 145.893 kb on + strand, within GFF424 at 145.894 kb on - strand, within GFF424 at 146.034 kb on + strand, within GFF424 at 146.062 kb on + strand, within GFF424 at 146.063 kb on - strand, within GFF424 at 146.110 kb on - strand, within GFF424 at 146.261 kb on - strand, within GFF424 at 146.262 kb on + strand, within GFF424 at 146.263 kb on - strand, within GFF424 at 146.263 kb on - strand, within GFF424 at 146.467 kb on + strand, within GFF424 at 146.476 kb on - strand, within GFF424 at 146.547 kb on + strand, within GFF424 at 146.548 kb on - strand, within GFF424 at 146.563 kb on + strand, within GFF424 at 146.564 kb on - strand, within GFF424 at 146.583 kb on - strand, within GFF424 at 146.583 kb on - strand, within GFF424 at 146.589 kb on + strand, within GFF424 at 146.589 kb on + strand, within GFF424 at 146.830 kb on - strand, within GFF424 at 146.833 kb on + strand, within GFF424 at 147.191 kb on - strand at 147.191 kb on - strand at 147.208 kb on - strand at 147.438 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 1.875 MOI remove 144,336 - GFF423 0.50 +0.0 144,392 - GFF423 0.54 -0.3 144,392 - GFF423 0.54 +0.2 144,470 - GFF423 0.59 -0.1 144,487 + GFF423 0.60 +1.2 144,487 + GFF423 0.60 -0.3 144,488 - GFF423 0.60 -0.5 144,525 - GFF423 0.62 +0.3 144,595 + GFF423 0.67 -0.1 144,647 + GFF423 0.70 -0.4 144,648 - GFF423 0.70 +0.1 144,649 + GFF423 0.70 -0.0 144,721 + GFF423 0.75 -0.5 144,734 + GFF423 0.76 -0.8 144,746 - GFF423 0.77 -0.2 144,769 + GFF423 0.78 +0.1 144,824 - GFF423 0.82 +0.3 144,960 - -0.3 144,998 - +0.0 145,167 - +0.2 145,333 - -0.3 145,403 - +1.0 145,466 - GFF424 0.12 +0.1 145,567 - GFF424 0.18 +0.2 145,718 + GFF424 0.25 -0.2 145,785 + GFF424 0.29 -0.1 145,851 + GFF424 0.32 -0.2 145,852 - GFF424 0.32 -0.3 145,852 - GFF424 0.32 -0.0 145,852 - GFF424 0.32 +0.6 145,855 + GFF424 0.32 -0.6 145,856 - GFF424 0.32 -0.7 145,863 + GFF424 0.33 -0.5 145,864 - GFF424 0.33 -0.0 145,893 + GFF424 0.34 +0.2 145,894 - GFF424 0.34 +0.6 146,034 + GFF424 0.41 -0.5 146,062 + GFF424 0.43 -0.5 146,063 - GFF424 0.43 +0.0 146,110 - GFF424 0.45 -0.7 146,261 - GFF424 0.53 -0.3 146,262 + GFF424 0.53 -0.1 146,263 - GFF424 0.53 +0.1 146,263 - GFF424 0.53 -0.2 146,467 + GFF424 0.64 +0.1 146,476 - GFF424 0.64 -0.1 146,547 + GFF424 0.68 -0.3 146,548 - GFF424 0.68 -0.3 146,563 + GFF424 0.69 +0.1 146,564 - GFF424 0.69 -0.7 146,583 - GFF424 0.70 -0.5 146,583 - GFF424 0.70 -0.8 146,589 + GFF424 0.70 +0.0 146,589 + GFF424 0.70 +0.0 146,830 - GFF424 0.82 +0.3 146,833 + GFF424 0.82 -0.6 147,191 - +1.2 147,191 - +0.2 147,208 - -0.0 147,438 - -0.2
Or see this region's nucleotide sequence