Experiment: LB_plus_SM_buffer with Ffm_phage 1.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4105 and GFF4106 overlap by 4 nucleotides GFF4106 and GFF4107 overlap by 4 nucleotides GFF4107 and GFF4108 are separated by 155 nucleotides GFF4108 and GFF4109 are separated by 248 nucleotides GFF4109 and GFF4110 are separated by 88 nucleotides
GFF4105 - Protein with similarity to RtcB, at 64,484 to 65,458
GFF4105
GFF4106 - Protein with similarity to RtcB, at 65,455 to 65,616
GFF4106
GFF4107 - Peptide chain release factor homolog, at 65,613 to 66,227
GFF4107
GFF4108 - Aminoacyl-histidine dipeptidase (Peptidase D) (EC 3.4.13.3), at 66,383 to 67,840
GFF4108
GFF4109 - Xanthine-guanine phosphoribosyltransferase (EC 2.4.2.22), at 68,089 to 68,547
GFF4109
GFF4110 - Fermentation/respiration switch protein, at 68,636 to 69,880
GFF4110
Position (kb)
66
67
68 Strain fitness (log2 ratio)
-1
0
1 at 65.482 kb on + strand, within GFF4106 at 65.483 kb on - strand, within GFF4106 at 65.503 kb on + strand, within GFF4106 at 65.503 kb on + strand, within GFF4106 at 65.506 kb on - strand, within GFF4106 at 65.717 kb on - strand, within GFF4107 at 65.753 kb on - strand, within GFF4107 at 65.753 kb on - strand, within GFF4107 at 65.923 kb on + strand, within GFF4107 at 65.987 kb on + strand, within GFF4107 at 65.988 kb on - strand, within GFF4107 at 66.201 kb on - strand at 66.249 kb on - strand at 66.286 kb on + strand at 66.310 kb on + strand at 66.452 kb on + strand at 66.452 kb on + strand at 66.452 kb on + strand at 66.453 kb on - strand at 66.709 kb on + strand, within GFF4108 at 66.710 kb on - strand, within GFF4108 at 66.710 kb on - strand, within GFF4108 at 66.710 kb on - strand, within GFF4108 at 66.994 kb on - strand, within GFF4108 at 67.229 kb on - strand, within GFF4108 at 67.312 kb on - strand, within GFF4108 at 67.312 kb on - strand, within GFF4108 at 67.450 kb on + strand, within GFF4108 at 67.451 kb on - strand, within GFF4108 at 67.451 kb on - strand, within GFF4108 at 67.543 kb on + strand, within GFF4108 at 67.543 kb on + strand, within GFF4108 at 67.544 kb on - strand, within GFF4108 at 67.544 kb on - strand, within GFF4108 at 67.544 kb on - strand, within GFF4108 at 67.544 kb on - strand, within GFF4108 at 67.545 kb on + strand, within GFF4108 at 67.546 kb on - strand, within GFF4108 at 67.546 kb on - strand, within GFF4108 at 67.546 kb on - strand, within GFF4108 at 67.546 kb on - strand, within GFF4108 at 67.556 kb on - strand, within GFF4108 at 67.649 kb on - strand, within GFF4108 at 67.704 kb on + strand at 67.705 kb on - strand at 67.778 kb on - strand at 67.778 kb on - strand at 67.843 kb on + strand at 67.843 kb on + strand at 67.843 kb on + strand at 67.844 kb on - strand at 67.911 kb on + strand at 67.911 kb on + strand at 67.911 kb on + strand at 67.955 kb on + strand at 67.958 kb on - strand at 67.958 kb on - strand at 68.034 kb on + strand at 68.034 kb on + strand at 68.035 kb on - strand at 68.035 kb on - strand at 68.276 kb on - strand, within GFF4109 at 68.399 kb on - strand, within GFF4109 at 68.462 kb on - strand, within GFF4109 at 68.467 kb on - strand, within GFF4109 at 68.602 kb on + strand at 68.602 kb on + strand at 68.617 kb on - strand at 68.698 kb on + strand at 68.788 kb on - strand, within GFF4110
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 1.875 MOI remove 65,482 + GFF4106 0.17 +0.1 65,483 - GFF4106 0.17 +0.5 65,503 + GFF4106 0.30 +0.2 65,503 + GFF4106 0.30 +0.2 65,506 - GFF4106 0.31 -0.2 65,717 - GFF4107 0.17 -0.3 65,753 - GFF4107 0.23 +0.6 65,753 - GFF4107 0.23 +0.6 65,923 + GFF4107 0.50 +0.2 65,987 + GFF4107 0.61 -0.1 65,988 - GFF4107 0.61 +1.4 66,201 - +0.3 66,249 - +1.3 66,286 + +0.6 66,310 + -0.1 66,452 + -0.1 66,452 + -0.3 66,452 + -0.2 66,453 - -0.1 66,709 + GFF4108 0.22 -0.6 66,710 - GFF4108 0.22 +0.1 66,710 - GFF4108 0.22 -0.2 66,710 - GFF4108 0.22 -1.6 66,994 - GFF4108 0.42 +0.4 67,229 - GFF4108 0.58 -0.5 67,312 - GFF4108 0.64 +0.1 67,312 - GFF4108 0.64 -1.4 67,450 + GFF4108 0.73 +0.2 67,451 - GFF4108 0.73 +0.0 67,451 - GFF4108 0.73 -0.1 67,543 + GFF4108 0.80 -1.1 67,543 + GFF4108 0.80 -0.1 67,544 - GFF4108 0.80 +0.5 67,544 - GFF4108 0.80 -0.3 67,544 - GFF4108 0.80 -0.6 67,544 - GFF4108 0.80 -0.5 67,545 + GFF4108 0.80 -0.4 67,546 - GFF4108 0.80 -0.8 67,546 - GFF4108 0.80 -0.4 67,546 - GFF4108 0.80 +0.2 67,546 - GFF4108 0.80 -0.2 67,556 - GFF4108 0.80 +0.0 67,649 - GFF4108 0.87 -0.3 67,704 + -0.1 67,705 - +0.3 67,778 - -0.5 67,778 - -0.0 67,843 + -0.7 67,843 + -0.1 67,843 + -0.5 67,844 - -0.6 67,911 + -0.4 67,911 + +0.2 67,911 + -0.7 67,955 + -0.0 67,958 - -0.2 67,958 - +0.3 68,034 + +0.5 68,034 + +0.2 68,035 - +0.4 68,035 - +0.4 68,276 - GFF4109 0.41 -0.8 68,399 - GFF4109 0.68 +0.2 68,462 - GFF4109 0.81 +1.2 68,467 - GFF4109 0.82 -0.0 68,602 + -0.8 68,602 + +0.5 68,617 - +0.4 68,698 + -0.1 68,788 - GFF4110 0.12 +0.3
Or see this region's nucleotide sequence