Experiment: LB_plus_SM_buffer with Ffm_phage 1.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4054 and GFF4055 are separated by 99 nucleotides GFF4055 and GFF4056 are separated by 23 nucleotides GFF4056 and GFF4057 are separated by 185 nucleotides GFF4057 and GFF4058 are separated by 253 nucleotides
GFF4054 - ClpB protein, at 16,604 to 19,243
GFF4054
GFF4055 - Uncharacterized protein ImpB, at 19,343 to 19,885
GFF4055
GFF4056 - Uncharacterized protein ImpC, at 19,909 to 21,417
GFF4056
GFF4057 - putative cytoplasmic protein, at 21,603 to 22,049
GFF4057
GFF4058 - Putative cytoplasmic protein USSDB7A, at 22,303 to 22,788
GFF4058
Position (kb) 19
20
21
22 Strain fitness (log2 ratio) -1
0
1 at 18.938 kb on + strand, within GFF4054 at 18.939 kb on - strand, within GFF4054 at 19.037 kb on + strand at 19.037 kb on + strand at 19.037 kb on + strand at 19.037 kb on + strand at 19.040 kb on - strand at 19.060 kb on + strand at 19.060 kb on + strand at 19.061 kb on - strand at 19.061 kb on - strand at 19.652 kb on - strand, within GFF4055 at 19.746 kb on - strand, within GFF4055 at 20.102 kb on - strand, within GFF4056 at 20.227 kb on + strand, within GFF4056 at 20.397 kb on + strand, within GFF4056 at 20.414 kb on - strand, within GFF4056 at 20.414 kb on - strand, within GFF4056 at 20.425 kb on - strand, within GFF4056 at 20.588 kb on - strand, within GFF4056 at 20.744 kb on - strand, within GFF4056 at 20.996 kb on - strand, within GFF4056 at 21.208 kb on + strand, within GFF4056 at 21.208 kb on + strand, within GFF4056 at 21.246 kb on + strand, within GFF4056 at 21.247 kb on - strand, within GFF4056 at 21.279 kb on - strand at 21.419 kb on - strand at 21.421 kb on - strand at 21.423 kb on - strand at 21.527 kb on - strand at 21.599 kb on + strand at 21.765 kb on + strand, within GFF4057 at 21.765 kb on + strand, within GFF4057 at 21.767 kb on + strand, within GFF4057 at 21.767 kb on + strand, within GFF4057 at 21.768 kb on - strand, within GFF4057 at 21.768 kb on - strand, within GFF4057 at 21.779 kb on - strand, within GFF4057 at 22.043 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 1.875 MOI remove 18,938 + GFF4054 0.88 -0.4 18,939 - GFF4054 0.88 +0.3 19,037 + -0.1 19,037 + +0.1 19,037 + -0.3 19,037 + -0.1 19,040 - +0.9 19,060 + +0.4 19,060 + -1.0 19,061 - -0.5 19,061 - -0.6 19,652 - GFF4055 0.57 -0.5 19,746 - GFF4055 0.74 -0.1 20,102 - GFF4056 0.13 -0.1 20,227 + GFF4056 0.21 -0.2 20,397 + GFF4056 0.32 +0.5 20,414 - GFF4056 0.33 -0.1 20,414 - GFF4056 0.33 -0.2 20,425 - GFF4056 0.34 +0.6 20,588 - GFF4056 0.45 +1.1 20,744 - GFF4056 0.55 +0.5 20,996 - GFF4056 0.72 +0.5 21,208 + GFF4056 0.86 +0.7 21,208 + GFF4056 0.86 +0.3 21,246 + GFF4056 0.89 -0.1 21,247 - GFF4056 0.89 +0.4 21,279 - +0.0 21,419 - +0.8 21,421 - +0.8 21,423 - -0.2 21,527 - -0.1 21,599 + -0.1 21,765 + GFF4057 0.36 +0.2 21,765 + GFF4057 0.36 -0.1 21,767 + GFF4057 0.37 -0.2 21,767 + GFF4057 0.37 -0.3 21,768 - GFF4057 0.37 -0.1 21,768 - GFF4057 0.37 -0.0 21,779 - GFF4057 0.39 +0.8 22,043 + -0.3
Or see this region's nucleotide sequence