Experiment: LB_plus_SM_buffer with Ffm_phage 1.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3866 and GFF3867 are separated by 5 nucleotides GFF3867 and GFF3868 overlap by 4 nucleotides GFF3868 and GFF3869 are separated by 86 nucleotides GFF3869 and GFF3870 are separated by 2 nucleotides
GFF3866 - Polysaccharide export lipoprotein Wza, at 142,286 to 143,341
GFF3866
GFF3867 - Low molecular weight protein-tyrosine-phosphatase Wzb (EC 3.1.3.48), at 143,347 to 143,796
GFF3867
GFF3868 - Tyrosine-protein kinase Wzc (EC 2.7.10.2), at 143,793 to 145,952
GFF3868
GFF3869 - Putative N-acetylgalactosaminyl-diphosphoundecaprenol glucuronosyltransferase, at 146,039 to 146,881
GFF3869
GFF3870 - Colanic acid biosynthesis acetyltransferase WcaB (EC 2.3.1.-), at 146,884 to 147,372
GFF3870
Position (kb) 143
144
145
146 Strain fitness (log2 ratio) -2
-1
0
1 at 142.943 kb on + strand, within GFF3866 at 142.944 kb on - strand, within GFF3866 at 142.944 kb on - strand, within GFF3866 at 143.165 kb on - strand, within GFF3866 at 143.217 kb on - strand, within GFF3866 at 143.572 kb on + strand, within GFF3867 at 143.572 kb on + strand, within GFF3867 at 143.649 kb on - strand, within GFF3867 at 143.735 kb on + strand, within GFF3867 at 143.908 kb on + strand at 143.933 kb on + strand at 144.355 kb on + strand, within GFF3868 at 144.422 kb on + strand, within GFF3868 at 144.423 kb on - strand, within GFF3868 at 144.658 kb on + strand, within GFF3868 at 144.946 kb on - strand, within GFF3868 at 144.946 kb on - strand, within GFF3868 at 144.947 kb on + strand, within GFF3868 at 144.948 kb on - strand, within GFF3868 at 144.948 kb on - strand, within GFF3868 at 145.103 kb on - strand, within GFF3868 at 145.188 kb on + strand, within GFF3868 at 145.254 kb on - strand, within GFF3868 at 145.367 kb on + strand, within GFF3868 at 145.373 kb on - strand, within GFF3868 at 145.466 kb on - strand, within GFF3868 at 145.535 kb on - strand, within GFF3868 at 145.564 kb on + strand, within GFF3868 at 145.911 kb on + strand at 145.932 kb on + strand at 145.933 kb on - strand at 145.990 kb on - strand at 146.125 kb on - strand, within GFF3869 at 146.136 kb on - strand, within GFF3869 at 146.231 kb on + strand, within GFF3869 at 146.303 kb on + strand, within GFF3869 at 146.304 kb on - strand, within GFF3869 at 146.399 kb on + strand, within GFF3869 at 146.411 kb on + strand, within GFF3869 at 146.411 kb on + strand, within GFF3869 at 146.488 kb on - strand, within GFF3869 at 146.506 kb on + strand, within GFF3869 at 146.524 kb on + strand, within GFF3869 at 146.525 kb on - strand, within GFF3869 at 146.586 kb on - strand, within GFF3869 at 146.659 kb on + strand, within GFF3869 at 146.660 kb on - strand, within GFF3869 at 146.784 kb on - strand, within GFF3869 at 146.846 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 1.875 MOI remove 142,943 + GFF3866 0.62 +0.4 142,944 - GFF3866 0.62 -0.1 142,944 - GFF3866 0.62 -0.7 143,165 - GFF3866 0.83 +0.4 143,217 - GFF3866 0.88 +1.0 143,572 + GFF3867 0.50 -0.5 143,572 + GFF3867 0.50 -1.2 143,649 - GFF3867 0.67 -0.5 143,735 + GFF3867 0.86 +0.3 143,908 + +0.4 143,933 + -0.2 144,355 + GFF3868 0.26 -0.8 144,422 + GFF3868 0.29 -0.1 144,423 - GFF3868 0.29 +0.2 144,658 + GFF3868 0.40 -0.3 144,946 - GFF3868 0.53 -0.1 144,946 - GFF3868 0.53 +0.2 144,947 + GFF3868 0.53 -0.4 144,948 - GFF3868 0.53 +0.4 144,948 - GFF3868 0.53 -0.2 145,103 - GFF3868 0.61 -0.5 145,188 + GFF3868 0.65 -0.3 145,254 - GFF3868 0.68 -0.7 145,367 + GFF3868 0.73 -0.8 145,373 - GFF3868 0.73 -0.3 145,466 - GFF3868 0.77 +0.9 145,535 - GFF3868 0.81 +0.2 145,564 + GFF3868 0.82 +0.2 145,911 + +0.3 145,932 + +0.3 145,933 - -2.3 145,990 - +0.0 146,125 - GFF3869 0.10 +0.0 146,136 - GFF3869 0.12 +0.0 146,231 + GFF3869 0.23 -0.4 146,303 + GFF3869 0.31 -0.2 146,304 - GFF3869 0.31 +0.1 146,399 + GFF3869 0.43 -1.5 146,411 + GFF3869 0.44 -0.4 146,411 + GFF3869 0.44 +0.3 146,488 - GFF3869 0.53 -0.2 146,506 + GFF3869 0.55 +0.1 146,524 + GFF3869 0.58 +0.1 146,525 - GFF3869 0.58 -0.2 146,586 - GFF3869 0.65 -0.9 146,659 + GFF3869 0.74 +0.7 146,660 - GFF3869 0.74 +1.0 146,784 - GFF3869 0.88 +0.7 146,846 - -0.4
Or see this region's nucleotide sequence