Experiment: LB_plus_SM_buffer with Ffm_phage 1.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3815 and GFF3816 overlap by 8 nucleotides GFF3816 and GFF3817 overlap by 17 nucleotides GFF3817 and GFF3818 are separated by 147 nucleotides
GFF3815 - Osmoprotectant ABC transporter permease protein YehY, at 84,781 to 85,950
GFF3815
GFF3816 - Osmoprotectant ABC transporter ATP-binding subunit YehX, at 85,943 to 86,890
GFF3816
GFF3817 - Osmoprotectant ABC transporter inner membrane protein YehW, at 86,874 to 87,605
GFF3817
GFF3818 - HTH-type transcriptional regulator mlrA, at 87,753 to 88,484
GFF3818
Position (kb) 86
87
88 Strain fitness (log2 ratio) -1
0
1 at 85.914 kb on - strand at 86.280 kb on + strand, within GFF3816 at 86.538 kb on + strand, within GFF3816 at 86.606 kb on - strand, within GFF3816 at 86.606 kb on - strand, within GFF3816 at 86.683 kb on - strand, within GFF3816 at 86.687 kb on - strand, within GFF3816 at 86.700 kb on - strand, within GFF3816 at 86.906 kb on + strand at 87.413 kb on + strand, within GFF3817 at 87.459 kb on - strand, within GFF3817 at 87.489 kb on - strand, within GFF3817 at 87.498 kb on - strand, within GFF3817 at 87.513 kb on - strand, within GFF3817 at 87.699 kb on - strand at 87.706 kb on - strand at 87.723 kb on - strand at 87.844 kb on - strand, within GFF3818 at 87.850 kb on - strand, within GFF3818 at 87.963 kb on - strand, within GFF3818 at 87.971 kb on + strand, within GFF3818 at 88.037 kb on + strand, within GFF3818 at 88.039 kb on + strand, within GFF3818 at 88.039 kb on + strand, within GFF3818 at 88.039 kb on + strand, within GFF3818 at 88.039 kb on + strand, within GFF3818 at 88.040 kb on - strand, within GFF3818 at 88.101 kb on + strand, within GFF3818 at 88.102 kb on - strand, within GFF3818 at 88.250 kb on + strand, within GFF3818 at 88.251 kb on - strand, within GFF3818 at 88.251 kb on - strand, within GFF3818 at 88.358 kb on + strand, within GFF3818 at 88.395 kb on + strand, within GFF3818 at 88.475 kb on - strand at 88.515 kb on + strand at 88.534 kb on - strand at 88.602 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 1.875 MOI remove 85,914 - -0.0 86,280 + GFF3816 0.36 -0.5 86,538 + GFF3816 0.63 +0.2 86,606 - GFF3816 0.70 +0.0 86,606 - GFF3816 0.70 +0.1 86,683 - GFF3816 0.78 -0.4 86,687 - GFF3816 0.78 -0.2 86,700 - GFF3816 0.80 +1.2 86,906 + -0.2 87,413 + GFF3817 0.74 +0.1 87,459 - GFF3817 0.80 -0.3 87,489 - GFF3817 0.84 -0.0 87,498 - GFF3817 0.85 -0.6 87,513 - GFF3817 0.87 -0.5 87,699 - -1.3 87,706 - -0.4 87,723 - +0.5 87,844 - GFF3818 0.12 -0.0 87,850 - GFF3818 0.13 +0.2 87,963 - GFF3818 0.29 -0.3 87,971 + GFF3818 0.30 +0.2 88,037 + GFF3818 0.39 +0.0 88,039 + GFF3818 0.39 +1.0 88,039 + GFF3818 0.39 -0.1 88,039 + GFF3818 0.39 +0.1 88,039 + GFF3818 0.39 -0.1 88,040 - GFF3818 0.39 -0.4 88,101 + GFF3818 0.48 +0.2 88,102 - GFF3818 0.48 +0.1 88,250 + GFF3818 0.68 -0.4 88,251 - GFF3818 0.68 -0.1 88,251 - GFF3818 0.68 -0.1 88,358 + GFF3818 0.83 +0.5 88,395 + GFF3818 0.88 -0.1 88,475 - -1.6 88,515 + +0.3 88,534 - -0.8 88,602 + +0.3
Or see this region's nucleotide sequence