Experiment: LB_plus_SM_buffer with Ffm_phage 1.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3562 and GFF3563 are separated by 12 nucleotides GFF3563 and GFF3564 are separated by 154 nucleotides GFF3564 and GFF3565 are separated by 66 nucleotides GFF3565 and GFF3566 are separated by 82 nucleotides GFF3566 and GFF3567 are separated by 310 nucleotides
GFF3562 - Cysteine desulfurase (EC 2.8.1.7), SufS subfamily, at 318,238 to 319,458
GFF3562
GFF3563 - Sulfur acceptor protein SufE for iron-sulfur cluster assembly, at 319,471 to 319,887
GFF3563
GFF3564 - L,D-transpeptidase YnhG, at 320,042 to 321,043
GFF3564
GFF3565 - major outer membrane lipoprotein, at 321,110 to 321,349
GFF3565
GFF3566 - major outer membrane lipoprotein, at 321,432 to 321,668
GFF3566
GFF3567 - Pyruvate kinase (EC 2.7.1.40), at 321,979 to 323,391
GFF3567
Position (kb) 320
321
322 Strain fitness (log2 ratio) -1
0
1 at 319.137 kb on + strand, within GFF3562 at 319.213 kb on + strand, within GFF3562 at 319.214 kb on - strand, within GFF3562 at 319.293 kb on + strand, within GFF3562 at 319.391 kb on - strand at 319.391 kb on - strand at 319.391 kb on - strand at 319.507 kb on - strand at 319.548 kb on + strand, within GFF3563 at 319.548 kb on + strand, within GFF3563 at 319.548 kb on + strand, within GFF3563 at 319.725 kb on - strand, within GFF3563 at 319.735 kb on + strand, within GFF3563 at 319.736 kb on - strand, within GFF3563 at 320.166 kb on - strand, within GFF3564 at 320.167 kb on + strand, within GFF3564 at 320.200 kb on + strand, within GFF3564 at 320.269 kb on + strand, within GFF3564 at 320.287 kb on + strand, within GFF3564 at 320.308 kb on - strand, within GFF3564 at 320.335 kb on + strand, within GFF3564 at 320.364 kb on - strand, within GFF3564 at 320.366 kb on + strand, within GFF3564 at 320.392 kb on - strand, within GFF3564 at 320.399 kb on + strand, within GFF3564 at 320.499 kb on + strand, within GFF3564 at 320.500 kb on - strand, within GFF3564 at 320.783 kb on + strand, within GFF3564 at 320.784 kb on - strand, within GFF3564 at 320.787 kb on + strand, within GFF3564 at 320.787 kb on + strand, within GFF3564 at 320.794 kb on - strand, within GFF3564 at 321.431 kb on - strand at 321.443 kb on - strand at 321.594 kb on - strand, within GFF3566 at 321.624 kb on - strand, within GFF3566 at 321.624 kb on - strand, within GFF3566 at 321.670 kb on - strand at 321.710 kb on - strand at 321.716 kb on - strand at 321.719 kb on - strand at 321.767 kb on - strand at 321.857 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 1.875 MOI remove 319,137 + GFF3562 0.74 +0.2 319,213 + GFF3562 0.80 +0.0 319,214 - GFF3562 0.80 +0.0 319,293 + GFF3562 0.86 -0.0 319,391 - +0.1 319,391 - -0.1 319,391 - -0.1 319,507 - +0.1 319,548 + GFF3563 0.18 -0.5 319,548 + GFF3563 0.18 -0.3 319,548 + GFF3563 0.18 -0.0 319,725 - GFF3563 0.61 +0.3 319,735 + GFF3563 0.63 +0.2 319,736 - GFF3563 0.64 -0.0 320,166 - GFF3564 0.12 +0.2 320,167 + GFF3564 0.12 -0.7 320,200 + GFF3564 0.16 +0.1 320,269 + GFF3564 0.23 -0.5 320,287 + GFF3564 0.24 -0.6 320,308 - GFF3564 0.27 -0.2 320,335 + GFF3564 0.29 -0.6 320,364 - GFF3564 0.32 -0.8 320,366 + GFF3564 0.32 +0.5 320,392 - GFF3564 0.35 -0.1 320,399 + GFF3564 0.36 -0.4 320,499 + GFF3564 0.46 -0.2 320,500 - GFF3564 0.46 -0.1 320,783 + GFF3564 0.74 -0.6 320,784 - GFF3564 0.74 +0.1 320,787 + GFF3564 0.74 -0.1 320,787 + GFF3564 0.74 +0.0 320,794 - GFF3564 0.75 +0.5 321,431 - -0.1 321,443 - -0.2 321,594 - GFF3566 0.68 +0.4 321,624 - GFF3566 0.81 -1.4 321,624 - GFF3566 0.81 +0.2 321,670 - +0.0 321,710 - -0.5 321,716 - -0.6 321,719 - +1.4 321,767 - -0.1 321,857 + -0.1
Or see this region's nucleotide sequence