Experiment: LB_plus_SM_buffer with Ffm_phage 1.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF350 and GFF351 are separated by 150 nucleotides GFF351 and GFF352 overlap by 36 nucleotides GFF352 and GFF353 are separated by 89 nucleotides
GFF350 - FIG01200701: possible membrane protein, at 68,605 to 70,284
GFF350
GFF351 - Xanthine permease, at 70,435 to 71,826
GFF351
GFF352 - FIG01048298: hypothetical protein, at 71,791 to 71,964
GFF352
GFF353 - Sodium/glutamate symport protein, at 72,054 to 73,259
GFF353
Position (kb)
70
71
72 Strain fitness (log2 ratio)
-1
0
1 at 69.556 kb on - strand, within GFF350 at 69.579 kb on + strand, within GFF350 at 69.661 kb on - strand, within GFF350 at 69.750 kb on + strand, within GFF350 at 69.987 kb on + strand, within GFF350 at 70.062 kb on + strand, within GFF350 at 70.063 kb on - strand, within GFF350 at 70.067 kb on - strand, within GFF350 at 70.127 kb on - strand at 70.127 kb on - strand at 70.199 kb on - strand at 70.247 kb on + strand at 70.268 kb on - strand at 70.287 kb on - strand at 70.349 kb on + strand at 70.349 kb on + strand at 70.350 kb on - strand at 70.398 kb on + strand at 70.635 kb on + strand, within GFF351 at 70.778 kb on + strand, within GFF351 at 70.846 kb on - strand, within GFF351 at 70.849 kb on - strand, within GFF351 at 70.914 kb on - strand, within GFF351 at 70.987 kb on - strand, within GFF351 at 71.045 kb on + strand, within GFF351 at 71.045 kb on + strand, within GFF351 at 71.046 kb on - strand, within GFF351 at 71.179 kb on - strand, within GFF351 at 71.511 kb on + strand, within GFF351 at 71.533 kb on + strand, within GFF351 at 71.677 kb on + strand, within GFF351 at 71.762 kb on - strand at 71.862 kb on - strand, within GFF352 at 72.040 kb on + strand at 72.041 kb on - strand at 72.041 kb on - strand at 72.052 kb on - strand at 72.152 kb on + strand at 72.182 kb on - strand, within GFF353 at 72.182 kb on - strand, within GFF353 at 72.243 kb on - strand, within GFF353 at 72.364 kb on + strand, within GFF353 at 72.365 kb on - strand, within GFF353 at 72.450 kb on - strand, within GFF353 at 72.597 kb on + strand, within GFF353 at 72.703 kb on + strand, within GFF353 at 72.729 kb on - strand, within GFF353 at 72.764 kb on - strand, within GFF353 at 72.809 kb on - strand, within GFF353 at 72.809 kb on - strand, within GFF353
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 1.875 MOI remove 69,556 - GFF350 0.57 -0.3 69,579 + GFF350 0.58 -0.2 69,661 - GFF350 0.63 +0.0 69,750 + GFF350 0.68 +0.4 69,987 + GFF350 0.82 -0.8 70,062 + GFF350 0.87 +1.0 70,063 - GFF350 0.87 +0.1 70,067 - GFF350 0.87 -0.3 70,127 - -0.1 70,127 - -0.1 70,199 - +0.3 70,247 + +0.0 70,268 - -0.3 70,287 - +0.1 70,349 + +0.7 70,349 + +0.1 70,350 - +0.2 70,398 + -0.2 70,635 + GFF351 0.14 +0.2 70,778 + GFF351 0.25 +0.0 70,846 - GFF351 0.30 +1.2 70,849 - GFF351 0.30 +0.7 70,914 - GFF351 0.34 +0.0 70,987 - GFF351 0.40 +0.2 71,045 + GFF351 0.44 +1.0 71,045 + GFF351 0.44 +0.2 71,046 - GFF351 0.44 -0.4 71,179 - GFF351 0.53 +0.0 71,511 + GFF351 0.77 +0.7 71,533 + GFF351 0.79 +0.0 71,677 + GFF351 0.89 +1.1 71,762 - -0.2 71,862 - GFF352 0.41 -0.3 72,040 + +0.2 72,041 - -0.1 72,041 - +1.1 72,052 - +1.7 72,152 + -0.6 72,182 - GFF353 0.11 +0.7 72,182 - GFF353 0.11 -0.2 72,243 - GFF353 0.16 -0.3 72,364 + GFF353 0.26 -0.2 72,365 - GFF353 0.26 +0.3 72,450 - GFF353 0.33 +0.6 72,597 + GFF353 0.45 -0.1 72,703 + GFF353 0.54 -0.3 72,729 - GFF353 0.56 -0.1 72,764 - GFF353 0.59 -0.4 72,809 - GFF353 0.63 +0.0 72,809 - GFF353 0.63 -0.5
Or see this region's nucleotide sequence