Experiment: LB_plus_SM_buffer with Ffm_phage 1.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3102 and GFF3103 overlap by 8 nucleotides GFF3103 and GFF3104 overlap by 8 nucleotides GFF3104 and GFF3105 overlap by 1 nucleotides GFF3105 and GFF3106 overlap by 4 nucleotides
GFF3102 - [NiFe] hydrogenase nickel incorporation protein HybF, at 112,613 to 112,954
GFF3102
GFF3103 - Hydrogenase-2 operon protein hybE, at 112,947 to 113,435
GFF3103
GFF3104 - Hydrogenase maturation protease (EC 3.4.24.-), at 113,428 to 113,922
GFF3104
GFF3105 - Uptake hydrogenase large subunit (EC 1.12.99.6), at 113,922 to 115,625
GFF3105
GFF3106 - Ni/Fe-hydrogenase 2 B-type cytochrome subunit, at 115,622 to 116,800
GFF3106
Position (kb)
113
114
115
116 Strain fitness (log2 ratio)
-1
0
1 at 113.031 kb on + strand, within GFF3103 at 113.325 kb on - strand, within GFF3103 at 113.834 kb on + strand, within GFF3104 at 113.911 kb on + strand at 113.924 kb on - strand at 114.015 kb on + strand at 114.016 kb on - strand at 114.098 kb on + strand, within GFF3105 at 114.099 kb on - strand, within GFF3105 at 114.139 kb on + strand, within GFF3105 at 114.139 kb on + strand, within GFF3105 at 114.144 kb on - strand, within GFF3105 at 114.150 kb on - strand, within GFF3105 at 114.278 kb on + strand, within GFF3105 at 114.282 kb on + strand, within GFF3105 at 114.283 kb on - strand, within GFF3105 at 114.298 kb on + strand, within GFF3105 at 114.312 kb on + strand, within GFF3105 at 114.312 kb on + strand, within GFF3105 at 114.366 kb on - strand, within GFF3105 at 114.542 kb on - strand, within GFF3105 at 114.542 kb on - strand, within GFF3105 at 114.542 kb on - strand, within GFF3105 at 114.713 kb on + strand, within GFF3105 at 114.718 kb on + strand, within GFF3105 at 114.820 kb on + strand, within GFF3105 at 114.852 kb on - strand, within GFF3105 at 114.854 kb on - strand, within GFF3105 at 114.901 kb on + strand, within GFF3105 at 114.926 kb on + strand, within GFF3105 at 114.983 kb on + strand, within GFF3105 at 115.054 kb on + strand, within GFF3105 at 115.054 kb on + strand, within GFF3105 at 115.130 kb on + strand, within GFF3105 at 115.131 kb on - strand, within GFF3105 at 115.135 kb on - strand, within GFF3105 at 115.346 kb on + strand, within GFF3105 at 115.356 kb on - strand, within GFF3105 at 115.357 kb on + strand, within GFF3105 at 115.357 kb on + strand, within GFF3105 at 115.358 kb on - strand, within GFF3105 at 115.449 kb on - strand, within GFF3105 at 115.449 kb on - strand, within GFF3105 at 115.608 kb on - strand at 115.704 kb on + strand at 115.704 kb on + strand at 115.704 kb on + strand at 115.705 kb on - strand at 115.705 kb on - strand at 115.735 kb on + strand at 115.864 kb on + strand, within GFF3106 at 115.876 kb on + strand, within GFF3106 at 115.990 kb on - strand, within GFF3106 at 116.045 kb on - strand, within GFF3106 at 116.123 kb on + strand, within GFF3106 at 116.124 kb on - strand, within GFF3106 at 116.184 kb on + strand, within GFF3106 at 116.211 kb on + strand, within GFF3106 at 116.379 kb on + strand, within GFF3106 at 116.379 kb on + strand, within GFF3106 at 116.379 kb on + strand, within GFF3106 at 116.379 kb on + strand, within GFF3106 at 116.380 kb on - strand, within GFF3106 at 116.442 kb on + strand, within GFF3106 at 116.442 kb on + strand, within GFF3106 at 116.443 kb on - strand, within GFF3106 at 116.448 kb on + strand, within GFF3106 at 116.480 kb on - strand, within GFF3106 at 116.551 kb on - strand, within GFF3106 at 116.572 kb on - strand, within GFF3106
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 1.875 MOI remove 113,031 + GFF3103 0.17 +0.9 113,325 - GFF3103 0.77 -0.0 113,834 + GFF3104 0.82 +0.2 113,911 + +0.0 113,924 - -0.1 114,015 + -0.0 114,016 - +0.3 114,098 + GFF3105 0.10 -0.1 114,099 - GFF3105 0.10 +0.1 114,139 + GFF3105 0.13 +0.4 114,139 + GFF3105 0.13 +0.3 114,144 - GFF3105 0.13 +1.1 114,150 - GFF3105 0.13 +0.1 114,278 + GFF3105 0.21 -0.3 114,282 + GFF3105 0.21 +0.2 114,283 - GFF3105 0.21 +0.3 114,298 + GFF3105 0.22 +0.7 114,312 + GFF3105 0.23 +0.0 114,312 + GFF3105 0.23 -0.6 114,366 - GFF3105 0.26 +0.4 114,542 - GFF3105 0.36 +1.0 114,542 - GFF3105 0.36 +0.4 114,542 - GFF3105 0.36 +0.2 114,713 + GFF3105 0.46 +0.3 114,718 + GFF3105 0.47 +0.4 114,820 + GFF3105 0.53 +0.3 114,852 - GFF3105 0.55 -0.0 114,854 - GFF3105 0.55 -0.2 114,901 + GFF3105 0.57 -0.1 114,926 + GFF3105 0.59 +0.7 114,983 + GFF3105 0.62 +0.4 115,054 + GFF3105 0.66 -0.0 115,054 + GFF3105 0.66 +0.0 115,130 + GFF3105 0.71 -0.3 115,131 - GFF3105 0.71 +0.1 115,135 - GFF3105 0.71 +0.7 115,346 + GFF3105 0.84 -0.2 115,356 - GFF3105 0.84 +0.5 115,357 + GFF3105 0.84 +0.4 115,357 + GFF3105 0.84 -0.2 115,358 - GFF3105 0.84 -0.8 115,449 - GFF3105 0.90 +0.1 115,449 - GFF3105 0.90 +0.4 115,608 - -0.5 115,704 + -0.0 115,704 + +0.0 115,704 + -0.5 115,705 - -0.2 115,705 - +0.0 115,735 + +0.4 115,864 + GFF3106 0.21 -0.0 115,876 + GFF3106 0.22 -0.7 115,990 - GFF3106 0.31 +0.5 116,045 - GFF3106 0.36 +0.6 116,123 + GFF3106 0.42 +0.3 116,124 - GFF3106 0.43 +0.3 116,184 + GFF3106 0.48 +0.2 116,211 + GFF3106 0.50 +0.2 116,379 + GFF3106 0.64 -0.2 116,379 + GFF3106 0.64 +0.1 116,379 + GFF3106 0.64 +0.2 116,379 + GFF3106 0.64 +1.0 116,380 - GFF3106 0.64 +0.1 116,442 + GFF3106 0.70 +0.1 116,442 + GFF3106 0.70 -1.5 116,443 - GFF3106 0.70 -1.0 116,448 + GFF3106 0.70 +0.2 116,480 - GFF3106 0.73 +0.0 116,551 - GFF3106 0.79 +0.3 116,572 - GFF3106 0.81 -0.3
Or see this region's nucleotide sequence