Experiment: LB_plus_SM_buffer with Ffm_phage 1.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3034 and GFF3035 are separated by 2 nucleotides GFF3035 and GFF3036 are separated by 810 nucleotides GFF3036 and GFF3037 overlap by 19 nucleotides
GFF3034 - Sorbitol dehydrogenase (EC 1.1.1.14), at 48,481 to 49,497
GFF3034
GFF3035 - D-mannonate oxidoreductase (EC 1.1.1.57), at 49,500 to 50,972
GFF3035
GFF3036 - Muconolactone isomerase (EC 5.3.3.4),putative, at 51,783 to 51,917
GFF3036
GFF3037 - hypothetical protein, at 51,899 to 52,015
GFF3037
Position (kb)
49
50
51 Strain fitness (log2 ratio)
-1
0
1 at 48.591 kb on + strand, within GFF3034 at 48.662 kb on - strand, within GFF3034 at 48.783 kb on + strand, within GFF3034 at 48.850 kb on - strand, within GFF3034 at 48.925 kb on - strand, within GFF3034 at 49.172 kb on + strand, within GFF3034 at 49.173 kb on - strand, within GFF3034 at 49.239 kb on + strand, within GFF3034 at 49.240 kb on - strand, within GFF3034 at 49.254 kb on + strand, within GFF3034 at 49.255 kb on - strand, within GFF3034 at 49.256 kb on + strand, within GFF3034 at 49.290 kb on + strand, within GFF3034 at 49.418 kb on - strand at 49.546 kb on - strand at 49.692 kb on - strand, within GFF3035 at 49.734 kb on - strand, within GFF3035 at 49.875 kb on - strand, within GFF3035 at 49.929 kb on + strand, within GFF3035 at 49.930 kb on - strand, within GFF3035 at 49.930 kb on - strand, within GFF3035 at 49.930 kb on - strand, within GFF3035 at 49.930 kb on - strand, within GFF3035 at 49.980 kb on + strand, within GFF3035 at 49.989 kb on + strand, within GFF3035 at 50.026 kb on + strand, within GFF3035 at 50.026 kb on + strand, within GFF3035 at 50.027 kb on - strand, within GFF3035 at 50.031 kb on - strand, within GFF3035 at 50.091 kb on - strand, within GFF3035 at 50.147 kb on + strand, within GFF3035 at 50.211 kb on + strand, within GFF3035 at 50.411 kb on + strand, within GFF3035 at 50.424 kb on + strand, within GFF3035 at 50.424 kb on + strand, within GFF3035 at 50.663 kb on + strand, within GFF3035 at 50.699 kb on + strand, within GFF3035 at 50.700 kb on - strand, within GFF3035 at 50.883 kb on - strand at 50.887 kb on - strand at 50.887 kb on - strand at 51.651 kb on + strand at 51.651 kb on + strand at 51.652 kb on - strand at 51.652 kb on - strand at 51.652 kb on - strand at 51.749 kb on + strand at 51.754 kb on - strand at 51.774 kb on - strand at 51.781 kb on - strand at 51.940 kb on + strand, within GFF3037 at 51.955 kb on - strand, within GFF3037
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 1.875 MOI remove 48,591 + GFF3034 0.11 +0.1 48,662 - GFF3034 0.18 +0.3 48,783 + GFF3034 0.30 +0.2 48,850 - GFF3034 0.36 +0.1 48,925 - GFF3034 0.44 +0.4 49,172 + GFF3034 0.68 -0.2 49,173 - GFF3034 0.68 +0.4 49,239 + GFF3034 0.75 +0.1 49,240 - GFF3034 0.75 +0.4 49,254 + GFF3034 0.76 -0.7 49,255 - GFF3034 0.76 +0.5 49,256 + GFF3034 0.76 +0.3 49,290 + GFF3034 0.80 -0.4 49,418 - +0.0 49,546 - +0.1 49,692 - GFF3035 0.13 -0.5 49,734 - GFF3035 0.16 +0.2 49,875 - GFF3035 0.25 -0.3 49,929 + GFF3035 0.29 -0.0 49,930 - GFF3035 0.29 +0.1 49,930 - GFF3035 0.29 -0.1 49,930 - GFF3035 0.29 -1.3 49,930 - GFF3035 0.29 +0.1 49,980 + GFF3035 0.33 -0.2 49,989 + GFF3035 0.33 +0.2 50,026 + GFF3035 0.36 -0.2 50,026 + GFF3035 0.36 -0.9 50,027 - GFF3035 0.36 +0.0 50,031 - GFF3035 0.36 -0.4 50,091 - GFF3035 0.40 -0.2 50,147 + GFF3035 0.44 -0.2 50,211 + GFF3035 0.48 +0.2 50,411 + GFF3035 0.62 +0.3 50,424 + GFF3035 0.63 +0.2 50,424 + GFF3035 0.63 -0.8 50,663 + GFF3035 0.79 -0.2 50,699 + GFF3035 0.81 +0.1 50,700 - GFF3035 0.81 +0.0 50,883 - +0.3 50,887 - +0.0 50,887 - +0.1 51,651 + +0.5 51,651 + +0.4 51,652 - +0.3 51,652 - +0.7 51,652 - -0.2 51,749 + +0.1 51,754 - +0.0 51,774 - -1.4 51,781 - -0.1 51,940 + GFF3037 0.35 -0.2 51,955 - GFF3037 0.48 +1.3
Or see this region's nucleotide sequence