Experiment: LB_plus_SM_buffer with Ffm_phage 1.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2955 and GFF2956 are separated by 671 nucleotides GFF2956 and GFF2957 are separated by 9 nucleotides
GFF2955 - COG1242: Predicted Fe-S oxidoreductase, at 34,398 to 35,327
GFF2955
GFF2956 - Glutamate synthase [NADPH] large chain (EC 1.4.1.13), at 35,999 to 40,459
GFF2956
GFF2957 - Glutamate synthase [NADPH] small chain (EC 1.4.1.13), at 40,469 to 41,887
GFF2957
Position (kb)
35
36
37
38
39
40
41 Strain fitness (log2 ratio)
-1
0
1 at 35.026 kb on + strand, within GFF2955 at 35.027 kb on - strand, within GFF2955 at 35.080 kb on + strand, within GFF2955 at 35.234 kb on + strand, within GFF2955 at 35.258 kb on + strand at 35.304 kb on - strand at 35.310 kb on + strand at 35.533 kb on + strand at 35.622 kb on + strand at 35.659 kb on - strand at 35.659 kb on - strand at 35.836 kb on - strand at 35.901 kb on + strand at 35.907 kb on + strand at 35.998 kb on + strand at 35.999 kb on - strand at 35.999 kb on - strand at 36.006 kb on - strand at 36.029 kb on - strand at 36.057 kb on + strand at 36.057 kb on + strand at 36.057 kb on + strand at 36.058 kb on - strand at 36.058 kb on - strand at 36.058 kb on - strand at 36.058 kb on - strand at 36.175 kb on - strand at 36.265 kb on + strand at 36.525 kb on + strand, within GFF2956 at 36.525 kb on + strand, within GFF2956 at 36.526 kb on - strand, within GFF2956 at 36.526 kb on - strand, within GFF2956 at 36.526 kb on - strand, within GFF2956 at 36.555 kb on - strand, within GFF2956 at 36.569 kb on - strand, within GFF2956 at 36.761 kb on - strand, within GFF2956 at 36.765 kb on - strand, within GFF2956 at 36.767 kb on - strand, within GFF2956 at 36.985 kb on + strand, within GFF2956 at 37.046 kb on - strand, within GFF2956 at 37.079 kb on + strand, within GFF2956 at 37.096 kb on + strand, within GFF2956 at 37.096 kb on + strand, within GFF2956 at 37.096 kb on + strand, within GFF2956 at 37.097 kb on - strand, within GFF2956 at 37.127 kb on - strand, within GFF2956 at 37.213 kb on + strand, within GFF2956 at 37.214 kb on - strand, within GFF2956 at 37.214 kb on - strand, within GFF2956 at 37.262 kb on + strand, within GFF2956 at 37.262 kb on + strand, within GFF2956 at 37.263 kb on - strand, within GFF2956 at 37.382 kb on - strand, within GFF2956 at 37.674 kb on - strand, within GFF2956 at 37.674 kb on - strand, within GFF2956 at 37.849 kb on + strand, within GFF2956 at 37.950 kb on + strand, within GFF2956 at 37.950 kb on + strand, within GFF2956 at 37.951 kb on - strand, within GFF2956 at 38.014 kb on + strand, within GFF2956 at 38.018 kb on + strand, within GFF2956 at 38.129 kb on + strand, within GFF2956 at 38.137 kb on + strand, within GFF2956 at 38.138 kb on - strand, within GFF2956 at 38.138 kb on - strand, within GFF2956 at 38.172 kb on - strand, within GFF2956 at 38.354 kb on + strand, within GFF2956 at 38.355 kb on - strand, within GFF2956 at 38.419 kb on - strand, within GFF2956 at 38.506 kb on - strand, within GFF2956 at 38.564 kb on - strand, within GFF2956 at 38.601 kb on + strand, within GFF2956 at 38.927 kb on - strand, within GFF2956 at 38.937 kb on + strand, within GFF2956 at 38.937 kb on + strand, within GFF2956 at 39.048 kb on - strand, within GFF2956 at 39.304 kb on + strand, within GFF2956 at 39.414 kb on - strand, within GFF2956 at 39.610 kb on + strand, within GFF2956 at 39.750 kb on - strand, within GFF2956 at 39.861 kb on + strand, within GFF2956 at 39.861 kb on + strand, within GFF2956 at 39.861 kb on + strand, within GFF2956 at 39.862 kb on - strand, within GFF2956 at 39.862 kb on - strand, within GFF2956 at 39.862 kb on - strand, within GFF2956 at 39.862 kb on - strand, within GFF2956 at 39.863 kb on + strand, within GFF2956 at 39.864 kb on - strand, within GFF2956 at 39.887 kb on + strand, within GFF2956 at 39.888 kb on - strand, within GFF2956 at 39.990 kb on - strand, within GFF2956 at 40.103 kb on + strand at 40.103 kb on + strand at 40.104 kb on - strand at 40.181 kb on + strand at 40.233 kb on + strand at 40.234 kb on - strand at 40.240 kb on - strand at 40.240 kb on - strand at 40.309 kb on + strand at 40.420 kb on + strand at 40.654 kb on + strand, within GFF2957 at 40.654 kb on + strand, within GFF2957 at 40.654 kb on + strand, within GFF2957 at 40.655 kb on - strand, within GFF2957 at 40.655 kb on - strand, within GFF2957 at 40.655 kb on - strand, within GFF2957 at 40.655 kb on - strand, within GFF2957 at 40.658 kb on + strand, within GFF2957 at 40.658 kb on + strand, within GFF2957 at 40.658 kb on + strand, within GFF2957 at 40.659 kb on - strand, within GFF2957 at 40.659 kb on - strand, within GFF2957 at 40.659 kb on - strand, within GFF2957 at 41.113 kb on + strand, within GFF2957 at 41.136 kb on + strand, within GFF2957 at 41.173 kb on - strand, within GFF2957 at 41.311 kb on + strand, within GFF2957 at 41.312 kb on + strand, within GFF2957 at 41.312 kb on + strand, within GFF2957 at 41.313 kb on - strand, within GFF2957 at 41.313 kb on - strand, within GFF2957 at 41.313 kb on - strand, within GFF2957 at 41.410 kb on - strand, within GFF2957 at 41.410 kb on - strand, within GFF2957
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 1.875 MOI remove 35,026 + GFF2955 0.68 +0.1 35,027 - GFF2955 0.68 +0.4 35,080 + GFF2955 0.73 -0.4 35,234 + GFF2955 0.90 -0.2 35,258 + +0.5 35,304 - +0.5 35,310 + +0.1 35,533 + -0.1 35,622 + +0.2 35,659 - +0.0 35,659 - -0.2 35,836 - +0.5 35,901 + +0.3 35,907 + +0.9 35,998 + -0.4 35,999 - +0.1 35,999 - +0.5 36,006 - +1.0 36,029 - -0.0 36,057 + +0.1 36,057 + +0.5 36,057 + -0.1 36,058 - +0.0 36,058 - -0.2 36,058 - -0.0 36,058 - +0.0 36,175 - +0.3 36,265 + -0.1 36,525 + GFF2956 0.12 -0.1 36,525 + GFF2956 0.12 -0.2 36,526 - GFF2956 0.12 +0.1 36,526 - GFF2956 0.12 +0.5 36,526 - GFF2956 0.12 -0.3 36,555 - GFF2956 0.12 -0.3 36,569 - GFF2956 0.13 -0.1 36,761 - GFF2956 0.17 +0.7 36,765 - GFF2956 0.17 +0.1 36,767 - GFF2956 0.17 +0.2 36,985 + GFF2956 0.22 +0.8 37,046 - GFF2956 0.23 +0.3 37,079 + GFF2956 0.24 +0.4 37,096 + GFF2956 0.25 +0.1 37,096 + GFF2956 0.25 +0.8 37,096 + GFF2956 0.25 +0.6 37,097 - GFF2956 0.25 +0.6 37,127 - GFF2956 0.25 -1.1 37,213 + GFF2956 0.27 -0.0 37,214 - GFF2956 0.27 -0.1 37,214 - GFF2956 0.27 -0.2 37,262 + GFF2956 0.28 +0.2 37,262 + GFF2956 0.28 +0.4 37,263 - GFF2956 0.28 +0.8 37,382 - GFF2956 0.31 +0.1 37,674 - GFF2956 0.38 +0.8 37,674 - GFF2956 0.38 -0.1 37,849 + GFF2956 0.41 +0.2 37,950 + GFF2956 0.44 +0.4 37,950 + GFF2956 0.44 +0.6 37,951 - GFF2956 0.44 +0.5 38,014 + GFF2956 0.45 -0.3 38,018 + GFF2956 0.45 -0.3 38,129 + GFF2956 0.48 +0.8 38,137 + GFF2956 0.48 +0.6 38,138 - GFF2956 0.48 +0.0 38,138 - GFF2956 0.48 +0.2 38,172 - GFF2956 0.49 -0.4 38,354 + GFF2956 0.53 +0.0 38,355 - GFF2956 0.53 +0.4 38,419 - GFF2956 0.54 +0.3 38,506 - GFF2956 0.56 +0.7 38,564 - GFF2956 0.57 +0.5 38,601 + GFF2956 0.58 +0.1 38,927 - GFF2956 0.66 +0.0 38,937 + GFF2956 0.66 +0.4 38,937 + GFF2956 0.66 +0.1 39,048 - GFF2956 0.68 -0.1 39,304 + GFF2956 0.74 -0.2 39,414 - GFF2956 0.77 +0.2 39,610 + GFF2956 0.81 +0.9 39,750 - GFF2956 0.84 +0.0 39,861 + GFF2956 0.87 +0.1 39,861 + GFF2956 0.87 +0.7 39,861 + GFF2956 0.87 +0.1 39,862 - GFF2956 0.87 -0.2 39,862 - GFF2956 0.87 +0.3 39,862 - GFF2956 0.87 +0.7 39,862 - GFF2956 0.87 +0.1 39,863 + GFF2956 0.87 +0.1 39,864 - GFF2956 0.87 +0.2 39,887 + GFF2956 0.87 +0.0 39,888 - GFF2956 0.87 +0.1 39,990 - GFF2956 0.89 -0.1 40,103 + +0.7 40,103 + +0.4 40,104 - +0.1 40,181 + -0.1 40,233 + -0.4 40,234 - -0.2 40,240 - -0.2 40,240 - +0.5 40,309 + +0.2 40,420 + +0.0 40,654 + GFF2957 0.13 +0.1 40,654 + GFF2957 0.13 +0.4 40,654 + GFF2957 0.13 +0.0 40,655 - GFF2957 0.13 -0.1 40,655 - GFF2957 0.13 -0.3 40,655 - GFF2957 0.13 +0.7 40,655 - GFF2957 0.13 -0.7 40,658 + GFF2957 0.13 -0.3 40,658 + GFF2957 0.13 +0.0 40,658 + GFF2957 0.13 -0.7 40,659 - GFF2957 0.13 +0.5 40,659 - GFF2957 0.13 +0.0 40,659 - GFF2957 0.13 +0.0 41,113 + GFF2957 0.45 +0.4 41,136 + GFF2957 0.47 -0.1 41,173 - GFF2957 0.50 -0.4 41,311 + GFF2957 0.59 -0.1 41,312 + GFF2957 0.59 -0.2 41,312 + GFF2957 0.59 +0.4 41,313 - GFF2957 0.59 +0.1 41,313 - GFF2957 0.59 +0.3 41,313 - GFF2957 0.59 -0.2 41,410 - GFF2957 0.66 +0.3 41,410 - GFF2957 0.66 +0.2
Or see this region's nucleotide sequence