Experiment: LB_plus_SM_buffer with Ffm_phage 1.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2941 and GFF2942 are separated by 20 nucleotides GFF2942 and GFF2943 overlap by 4 nucleotides GFF2943 and GFF2944 overlap by 32 nucleotides GFF2944 and GFF2945 are separated by 6 nucleotides
GFF2941 - Arabinose 5-phosphate isomerase (EC 5.3.1.13), at 22,438 to 23,424
GFF2941
GFF2942 - 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase (EC 3.1.3.45), at 23,445 to 24,011
GFF2942
GFF2943 - Uncharacterized protein YrbK clustered with lipopolysaccharide transporters, at 24,008 to 24,583
GFF2943
GFF2944 - LptA, protein essential for LPS transport across the periplasm, at 24,552 to 25,106
GFF2944
GFF2945 - Lipopolysaccharide ABC transporter, ATP-binding protein LptB, at 25,113 to 25,838
GFF2945
Position (kb) 24
25 Strain fitness (log2 ratio) -1
0
1 at 23.042 kb on + strand, within GFF2941 at 23.391 kb on + strand at 23.391 kb on + strand at 23.391 kb on + strand at 23.571 kb on + strand, within GFF2942 at 23.571 kb on + strand, within GFF2942 at 23.808 kb on + strand, within GFF2942 at 24.436 kb on + strand, within GFF2943 at 24.436 kb on + strand, within GFF2943 at 24.459 kb on + strand, within GFF2943
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 1.875 MOI remove 23,042 + GFF2941 0.61 +0.2 23,391 + +0.4 23,391 + -0.4 23,391 + -0.3 23,571 + GFF2942 0.22 +0.1 23,571 + GFF2942 0.22 +0.0 23,808 + GFF2942 0.64 -0.3 24,436 + GFF2943 0.74 +0.8 24,436 + GFF2943 0.74 -0.8 24,459 + GFF2943 0.78 -1.3
Or see this region's nucleotide sequence