Experiment: LB_plus_SM_buffer with Ffm_phage 1.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2850 and GFF2851 overlap by 29 nucleotides GFF2851 and GFF2852 are separated by 11 nucleotides GFF2852 and GFF2853 are separated by 167 nucleotides GFF2853 and GFF2854 are separated by 18 nucleotides GFF2854 and GFF2855 are separated by 17 nucleotides
GFF2850 - Ribosomal RNA small subunit methyltransferase F (EC 2.1.1.-), at 373,745 to 375,286
GFF2850
GFF2851 - hypothetical protein, at 375,258 to 375,389
GFF2851
GFF2852 - FIG00638105: hypothetical protein, at 375,401 to 375,640
GFF2852
GFF2853 - FIG00638765: hypothetical protein, at 375,808 to 375,942
GFF2853
GFF2854 - Ren protein, at 375,961 to 376,611
GFF2854
GFF2855 - FIG01046004: hypothetical protein, at 376,629 to 376,802
GFF2855
Position (kb) 375
376 Strain fitness (log2 ratio) -1
0
1 at 374.455 kb on - strand, within GFF2850 at 374.459 kb on - strand, within GFF2850 at 374.639 kb on + strand, within GFF2850 at 374.640 kb on - strand, within GFF2850 at 374.640 kb on - strand, within GFF2850 at 374.810 kb on + strand, within GFF2850 at 375.020 kb on - strand, within GFF2850 at 375.281 kb on - strand at 375.330 kb on + strand, within GFF2851 at 375.453 kb on + strand, within GFF2852 at 375.496 kb on + strand, within GFF2852 at 375.513 kb on + strand, within GFF2852 at 375.600 kb on - strand, within GFF2852 at 375.608 kb on - strand, within GFF2852 at 375.715 kb on - strand at 375.732 kb on - strand at 375.732 kb on - strand at 375.735 kb on + strand at 375.749 kb on + strand at 375.750 kb on - strand at 375.817 kb on + strand at 376.014 kb on + strand at 376.017 kb on - strand at 376.017 kb on - strand at 376.163 kb on + strand, within GFF2854
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 1.875 MOI remove 374,455 - GFF2850 0.46 +0.0 374,459 - GFF2850 0.46 +0.2 374,639 + GFF2850 0.58 +0.8 374,640 - GFF2850 0.58 +0.4 374,640 - GFF2850 0.58 -1.4 374,810 + GFF2850 0.69 +0.5 375,020 - GFF2850 0.83 +0.1 375,281 - +0.4 375,330 + GFF2851 0.55 -0.0 375,453 + GFF2852 0.22 +1.0 375,496 + GFF2852 0.40 +0.5 375,513 + GFF2852 0.47 +0.5 375,600 - GFF2852 0.83 +0.6 375,608 - GFF2852 0.86 +0.0 375,715 - +0.5 375,732 - +0.6 375,732 - -0.2 375,735 + +0.4 375,749 + +0.0 375,750 - +0.3 375,817 + -0.3 376,014 + -0.5 376,017 - +0.1 376,017 - +0.4 376,163 + GFF2854 0.31 +0.1
Or see this region's nucleotide sequence