Experiment: LB_plus_SM_buffer with Ffm_phage 1.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2739 and GFF2740 are separated by 33 nucleotides GFF2740 and GFF2741 are separated by 47 nucleotides GFF2741 and GFF2742 are separated by 47 nucleotides
GFF2739 - Cardiolipin synthetase (EC 2.7.8.-), at 258,486 to 259,946
GFF2739
GFF2740 - FIG002901: hypothetical protein co-occurring with Cardiolipin synthetase, at 259,980 to 260,309
GFF2740
GFF2741 - Potassium voltage-gated channel subfamily KQT; possible potassium channel, VIC family, at 260,357 to 261,193
GFF2741
GFF2742 - Oligopeptide transport ATP-binding protein OppF (TC 3.A.1.5.1), at 261,241 to 262,245
GFF2742
Position (kb) 259
260
261 Strain fitness (log2 ratio) -1
0
1 at 259.182 kb on + strand, within GFF2739 at 259.340 kb on + strand, within GFF2739 at 259.376 kb on + strand, within GFF2739 at 259.377 kb on - strand, within GFF2739 at 259.477 kb on - strand, within GFF2739 at 259.544 kb on + strand, within GFF2739 at 259.584 kb on - strand, within GFF2739 at 259.693 kb on + strand, within GFF2739 at 259.693 kb on + strand, within GFF2739 at 259.784 kb on + strand, within GFF2739 at 259.864 kb on + strand at 259.905 kb on - strand at 260.387 kb on - strand at 260.514 kb on + strand, within GFF2741 at 260.515 kb on - strand, within GFF2741 at 260.546 kb on - strand, within GFF2741 at 260.546 kb on - strand, within GFF2741 at 260.627 kb on - strand, within GFF2741 at 260.659 kb on + strand, within GFF2741 at 260.664 kb on + strand, within GFF2741 at 260.665 kb on - strand, within GFF2741 at 261.077 kb on + strand, within GFF2741
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 1.875 MOI remove 259,182 + GFF2739 0.48 -0.7 259,340 + GFF2739 0.58 -0.2 259,376 + GFF2739 0.61 +0.2 259,377 - GFF2739 0.61 +1.3 259,477 - GFF2739 0.68 +1.2 259,544 + GFF2739 0.72 +0.3 259,584 - GFF2739 0.75 -0.9 259,693 + GFF2739 0.83 -0.5 259,693 + GFF2739 0.83 +0.0 259,784 + GFF2739 0.89 +0.4 259,864 + +0.3 259,905 - +0.0 260,387 - -0.1 260,514 + GFF2741 0.19 -0.5 260,515 - GFF2741 0.19 -0.7 260,546 - GFF2741 0.23 -0.4 260,546 - GFF2741 0.23 -0.2 260,627 - GFF2741 0.32 +0.4 260,659 + GFF2741 0.36 -1.1 260,664 + GFF2741 0.37 -0.5 260,665 - GFF2741 0.37 -0.1 261,077 + GFF2741 0.86 +0.2
Or see this region's nucleotide sequence