Experiment: LB_plus_SM_buffer with Ffm_phage 1.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF270 and GFF271 are separated by 36 nucleotides GFF271 and GFF272 are separated by 196 nucleotides
GFF270 - Possible transferase, at 44,951 to 45,664
GFF270
GFF271 - Possible transferase, at 45,701 to 46,774
GFF271
GFF272 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16) @ Acetyl-CoA acetyltransferase (EC 2.3.1.9), at 46,971 to 48,134
GFF272
Position (kb) 45
46
47 Strain fitness (log2 ratio) -1
0
1 at 45.016 kb on + strand at 45.059 kb on + strand, within GFF270 at 45.092 kb on - strand, within GFF270 at 45.151 kb on - strand, within GFF270 at 45.206 kb on - strand, within GFF270 at 45.314 kb on - strand, within GFF270 at 45.615 kb on + strand at 45.640 kb on + strand at 45.699 kb on - strand at 45.699 kb on - strand at 45.755 kb on + strand at 45.755 kb on + strand at 45.800 kb on + strand at 45.801 kb on - strand at 45.824 kb on - strand, within GFF271 at 45.828 kb on - strand, within GFF271 at 45.829 kb on + strand, within GFF271 at 45.901 kb on - strand, within GFF271 at 46.012 kb on + strand, within GFF271 at 46.013 kb on - strand, within GFF271 at 46.118 kb on + strand, within GFF271 at 46.357 kb on + strand, within GFF271 at 46.358 kb on - strand, within GFF271 at 46.360 kb on + strand, within GFF271 at 46.360 kb on + strand, within GFF271 at 46.361 kb on - strand, within GFF271 at 46.376 kb on - strand, within GFF271 at 46.380 kb on + strand, within GFF271 at 46.380 kb on + strand, within GFF271 at 46.627 kb on - strand, within GFF271 at 46.654 kb on + strand, within GFF271 at 46.713 kb on + strand at 46.713 kb on + strand at 46.713 kb on + strand at 46.738 kb on + strand at 46.738 kb on + strand at 46.828 kb on + strand at 46.893 kb on + strand at 46.893 kb on + strand at 46.893 kb on + strand at 46.894 kb on - strand at 46.894 kb on - strand at 46.894 kb on - strand at 46.894 kb on - strand at 46.894 kb on - strand at 46.897 kb on + strand at 46.897 kb on + strand at 46.897 kb on + strand at 46.897 kb on + strand at 46.897 kb on + strand at 46.898 kb on - strand at 46.898 kb on - strand at 46.898 kb on - strand at 46.898 kb on - strand at 46.898 kb on - strand at 46.898 kb on - strand at 46.898 kb on - strand at 46.901 kb on + strand at 46.902 kb on - strand at 46.902 kb on - strand at 46.902 kb on - strand at 46.962 kb on + strand at 46.963 kb on - strand at 47.015 kb on + strand at 47.016 kb on - strand at 47.016 kb on - strand at 47.385 kb on + strand, within GFF272 at 47.519 kb on - strand, within GFF272 at 47.519 kb on - strand, within GFF272
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 1.875 MOI remove 45,016 + +0.1 45,059 + GFF270 0.15 -0.1 45,092 - GFF270 0.20 +0.1 45,151 - GFF270 0.28 +0.4 45,206 - GFF270 0.36 +0.5 45,314 - GFF270 0.51 +0.2 45,615 + -0.7 45,640 + +0.1 45,699 - -0.3 45,699 - +0.4 45,755 + -0.3 45,755 + +0.2 45,800 + +0.0 45,801 - -0.7 45,824 - GFF271 0.11 -0.2 45,828 - GFF271 0.12 +0.8 45,829 + GFF271 0.12 +0.0 45,901 - GFF271 0.19 -0.8 46,012 + GFF271 0.29 -0.1 46,013 - GFF271 0.29 +0.3 46,118 + GFF271 0.39 +1.1 46,357 + GFF271 0.61 +0.5 46,358 - GFF271 0.61 -0.2 46,360 + GFF271 0.61 -0.2 46,360 + GFF271 0.61 +0.6 46,361 - GFF271 0.61 +0.2 46,376 - GFF271 0.63 +0.5 46,380 + GFF271 0.63 -0.1 46,380 + GFF271 0.63 -0.0 46,627 - GFF271 0.86 +0.5 46,654 + GFF271 0.89 +0.1 46,713 + -0.1 46,713 + -0.1 46,713 + +0.6 46,738 + +0.3 46,738 + +0.4 46,828 + +0.2 46,893 + +0.4 46,893 + +0.3 46,893 + +0.9 46,894 - +0.0 46,894 - +0.1 46,894 - +0.1 46,894 - +0.1 46,894 - +0.1 46,897 + +0.6 46,897 + +0.2 46,897 + +0.5 46,897 + +0.2 46,897 + +0.4 46,898 - -0.4 46,898 - +0.4 46,898 - -0.1 46,898 - +0.0 46,898 - +1.1 46,898 - -0.2 46,898 - +0.5 46,901 + -0.2 46,902 - +0.2 46,902 - -0.0 46,902 - +0.3 46,962 + +0.6 46,963 - -0.1 47,015 + +0.2 47,016 - +0.6 47,016 - -0.9 47,385 + GFF272 0.36 +0.9 47,519 - GFF272 0.47 +0.1 47,519 - GFF272 0.47 +0.6
Or see this region's nucleotide sequence