Experiment: LB_plus_SM_buffer with Ffm_phage 1.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF265 and GFF266 are separated by 29 nucleotides GFF266 and GFF267 are separated by 7 nucleotides GFF267 and GFF268 are separated by 185 nucleotides
GFF265 - Twin-arginine translocation protein TatC, at 40,147 to 40,926
GFF265
GFF266 - Deoxyribonuclease TatD, at 40,956 to 41,750
GFF266
GFF267 - Transcriptional activator RfaH, at 41,758 to 42,246
GFF267
GFF268 - 3-polyprenyl-4-hydroxybenzoate carboxy-lyase (EC 4.1.1.-), at 42,432 to 43,910
GFF268
Position (kb) 41
42
43 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 40.814 kb on - strand, within GFF265 at 40.852 kb on + strand at 40.853 kb on - strand at 41.061 kb on - strand, within GFF266 at 41.066 kb on - strand, within GFF266 at 41.066 kb on - strand, within GFF266 at 41.066 kb on - strand, within GFF266 at 41.198 kb on + strand, within GFF266 at 41.199 kb on - strand, within GFF266 at 41.308 kb on + strand, within GFF266 at 41.342 kb on + strand, within GFF266 at 41.476 kb on - strand, within GFF266 at 41.476 kb on - strand, within GFF266 at 41.476 kb on - strand, within GFF266 at 41.476 kb on - strand, within GFF266 at 41.524 kb on + strand, within GFF266 at 41.672 kb on - strand at 41.970 kb on + strand, within GFF267 at 42.045 kb on + strand, within GFF267 at 42.045 kb on + strand, within GFF267 at 42.046 kb on - strand, within GFF267 at 42.051 kb on + strand, within GFF267 at 42.051 kb on + strand, within GFF267 at 42.051 kb on + strand, within GFF267 at 42.052 kb on - strand, within GFF267 at 42.056 kb on - strand, within GFF267 at 42.117 kb on + strand, within GFF267 at 42.170 kb on - strand, within GFF267 at 42.170 kb on - strand, within GFF267 at 42.170 kb on - strand, within GFF267 at 42.224 kb on + strand at 42.229 kb on + strand at 42.233 kb on + strand at 42.246 kb on + strand at 42.247 kb on - strand at 42.303 kb on + strand at 42.308 kb on - strand at 42.309 kb on + strand at 42.310 kb on - strand at 42.310 kb on - strand at 42.405 kb on + strand at 42.405 kb on + strand at 42.418 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 1.875 MOI remove 40,814 - GFF265 0.86 -0.5 40,852 + -0.1 40,853 - +0.2 41,061 - GFF266 0.13 +1.2 41,066 - GFF266 0.14 +0.6 41,066 - GFF266 0.14 -0.5 41,066 - GFF266 0.14 +0.5 41,198 + GFF266 0.30 +0.1 41,199 - GFF266 0.31 -0.1 41,308 + GFF266 0.44 -0.2 41,342 + GFF266 0.49 -0.1 41,476 - GFF266 0.65 -0.4 41,476 - GFF266 0.65 +0.1 41,476 - GFF266 0.65 +0.3 41,476 - GFF266 0.65 +0.4 41,524 + GFF266 0.71 +0.3 41,672 - -0.1 41,970 + GFF267 0.43 -1.1 42,045 + GFF267 0.59 -0.1 42,045 + GFF267 0.59 -2.0 42,046 - GFF267 0.59 -0.6 42,051 + GFF267 0.60 -0.5 42,051 + GFF267 0.60 -2.0 42,051 + GFF267 0.60 -2.9 42,052 - GFF267 0.60 -1.0 42,056 - GFF267 0.61 -0.9 42,117 + GFF267 0.73 -0.3 42,170 - GFF267 0.84 +0.6 42,170 - GFF267 0.84 -0.4 42,170 - GFF267 0.84 +0.3 42,224 + -0.7 42,229 + -1.6 42,233 + +0.2 42,246 + +0.2 42,247 - +0.1 42,303 + -0.1 42,308 - +0.7 42,309 + +0.2 42,310 - -0.3 42,310 - +0.1 42,405 + +0.0 42,405 + -0.1 42,418 - -0.2
Or see this region's nucleotide sequence