Experiment: LB_plus_SM_buffer with Ffm_phage 1.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2602 and GFF2603 overlap by 4 nucleotides GFF2603 and GFF2604 are separated by 10 nucleotides GFF2604 and GFF2605 are separated by 13 nucleotides GFF2605 and GFF2606 are separated by 131 nucleotides
GFF2602 - Putative sgc region protein sgcX, at 127,416 to 128,534
GFF2602
GFF2603 - Putative phosphotransferase enzyme, at 128,531 to 128,812
GFF2603
GFF2604 - PTS system, galactitol-specific IIC component (EC 2.7.1.69), at 128,823 to 130,136
GFF2604
GFF2605 - Putative nucleoside triphosphatase, at 130,150 to 130,956
GFF2605
GFF2606 - PTS system, IIA component, at 131,088 to 131,525
GFF2606
Position (kb) 128
129
130
131 Strain fitness (log2 ratio) -1
0
1 at 127.936 kb on + strand, within GFF2602 at 127.937 kb on - strand, within GFF2602 at 128.114 kb on + strand, within GFF2602 at 128.205 kb on + strand, within GFF2602 at 128.396 kb on + strand, within GFF2602 at 128.452 kb on + strand at 128.890 kb on + strand at 128.901 kb on - strand at 129.176 kb on + strand, within GFF2604 at 129.180 kb on + strand, within GFF2604 at 129.180 kb on + strand, within GFF2604 at 129.195 kb on + strand, within GFF2604 at 129.385 kb on + strand, within GFF2604 at 129.400 kb on + strand, within GFF2604 at 129.400 kb on + strand, within GFF2604 at 129.539 kb on + strand, within GFF2604 at 129.622 kb on + strand, within GFF2604 at 129.763 kb on + strand, within GFF2604 at 129.764 kb on - strand, within GFF2604 at 129.876 kb on - strand, within GFF2604 at 129.905 kb on + strand, within GFF2604 at 129.906 kb on - strand, within GFF2604 at 129.940 kb on - strand, within GFF2604 at 130.019 kb on + strand at 130.101 kb on + strand at 130.102 kb on + strand at 130.102 kb on + strand at 130.111 kb on + strand at 130.115 kb on + strand at 130.348 kb on + strand, within GFF2605 at 131.103 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 1.875 MOI remove 127,936 + GFF2602 0.46 +0.8 127,937 - GFF2602 0.47 -0.2 128,114 + GFF2602 0.62 +0.1 128,205 + GFF2602 0.71 +0.0 128,396 + GFF2602 0.88 +0.2 128,452 + +0.1 128,890 + +1.1 128,901 - +0.2 129,176 + GFF2604 0.27 +0.1 129,180 + GFF2604 0.27 -0.2 129,180 + GFF2604 0.27 +0.1 129,195 + GFF2604 0.28 -0.6 129,385 + GFF2604 0.43 -1.5 129,400 + GFF2604 0.44 +0.4 129,400 + GFF2604 0.44 -0.6 129,539 + GFF2604 0.54 -0.0 129,622 + GFF2604 0.61 -0.2 129,763 + GFF2604 0.72 +0.1 129,764 - GFF2604 0.72 -0.4 129,876 - GFF2604 0.80 -0.1 129,905 + GFF2604 0.82 -0.6 129,906 - GFF2604 0.82 -0.5 129,940 - GFF2604 0.85 +0.5 130,019 + -0.2 130,101 + +0.0 130,102 + -0.4 130,102 + +0.1 130,111 + -0.0 130,115 + -0.2 130,348 + GFF2605 0.25 +1.2 131,103 - -0.2
Or see this region's nucleotide sequence