Experiment: LB_plus_SM_buffer with Ffm_phage 1.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2466 and GFF2467 are separated by 38 nucleotides GFF2467 and GFF2468 are separated by 21 nucleotides GFF2468 and GFF2469 are separated by 115 nucleotides
GFF2466 - Sialic acid utilization regulator, RpiR family, at 86,073 to 86,921
GFF2466
GFF2467 - 2-dehydropantoate 2-reductase (EC 1.1.1.169), at 86,960 to 87,877
GFF2467
GFF2468 - Putative transmembrane transport protein, at 87,899 to 89,095
GFF2468
GFF2469 - Transcriptional regulator, LysR family, at 89,211 to 90,137
GFF2469
Position (kb) 87
88
89
90 Strain fitness (log2 ratio) -1
0
1 at 86.962 kb on - strand at 87.019 kb on + strand at 87.020 kb on - strand at 87.056 kb on + strand, within GFF2467 at 87.057 kb on - strand, within GFF2467 at 87.089 kb on + strand, within GFF2467 at 87.089 kb on + strand, within GFF2467 at 87.104 kb on - strand, within GFF2467 at 87.104 kb on - strand, within GFF2467 at 87.104 kb on - strand, within GFF2467 at 87.274 kb on + strand, within GFF2467 at 87.295 kb on - strand, within GFF2467 at 87.345 kb on - strand, within GFF2467 at 87.379 kb on + strand, within GFF2467 at 87.572 kb on - strand, within GFF2467 at 87.617 kb on - strand, within GFF2467 at 87.632 kb on - strand, within GFF2467 at 87.718 kb on - strand, within GFF2467 at 87.718 kb on - strand, within GFF2467 at 87.794 kb on - strand at 88.251 kb on + strand, within GFF2468 at 88.983 kb on + strand at 89.484 kb on - strand, within GFF2469 at 89.801 kb on - strand, within GFF2469 at 90.082 kb on + strand at 90.083 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 1.875 MOI remove 86,962 - +0.5 87,019 + +1.0 87,020 - -0.3 87,056 + GFF2467 0.10 +0.5 87,057 - GFF2467 0.11 -0.2 87,089 + GFF2467 0.14 +0.4 87,089 + GFF2467 0.14 -1.6 87,104 - GFF2467 0.16 +0.5 87,104 - GFF2467 0.16 -0.1 87,104 - GFF2467 0.16 -0.2 87,274 + GFF2467 0.34 +0.1 87,295 - GFF2467 0.36 +0.7 87,345 - GFF2467 0.42 +0.2 87,379 + GFF2467 0.46 +0.1 87,572 - GFF2467 0.67 +1.1 87,617 - GFF2467 0.72 +0.6 87,632 - GFF2467 0.73 -0.1 87,718 - GFF2467 0.83 -0.0 87,718 - GFF2467 0.83 -0.0 87,794 - -0.3 88,251 + GFF2468 0.29 -0.6 88,983 + -0.4 89,484 - GFF2469 0.29 -0.3 89,801 - GFF2469 0.64 +0.6 90,082 + +0.3 90,083 - +0.1
Or see this region's nucleotide sequence