Experiment: LB_plus_SM_buffer with Ffm_phage 1.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2204 and GFF2205 are separated by 10 nucleotides GFF2205 and GFF2206 overlap by 4 nucleotides GFF2206 and GFF2207 are separated by 215 nucleotides GFF2207 and GFF2208 are separated by 291 nucleotides
GFF2204 - DNA replication protein DnaC, at 2,797 to 3,546
GFF2204
GFF2205 - putative DNA-binding protein, at 3,557 to 3,904
GFF2205
GFF2206 - FIG01045224: hypothetical protein, at 3,901 to 4,212
GFF2206
GFF2207 - hypothetical protein, at 4,428 to 4,580
GFF2207
GFF2208 - DNA-damage-inducible protein I, at 4,872 to 5,105
GFF2208
Position (kb) 4
5 Strain fitness (log2 ratio) -2
-1
0
1 at 3.508 kb on + strand at 3.544 kb on + strand at 3.692 kb on + strand, within GFF2205 at 3.692 kb on + strand, within GFF2205 at 3.693 kb on - strand, within GFF2205 at 3.693 kb on - strand, within GFF2205 at 3.799 kb on + strand, within GFF2205 at 3.800 kb on - strand, within GFF2205 at 3.850 kb on - strand, within GFF2205 at 3.948 kb on + strand, within GFF2206 at 4.042 kb on - strand, within GFF2206 at 4.154 kb on - strand, within GFF2206 at 4.213 kb on + strand at 4.213 kb on + strand at 4.213 kb on + strand at 4.251 kb on + strand at 4.255 kb on + strand at 4.256 kb on - strand at 4.356 kb on + strand at 4.356 kb on + strand at 4.390 kb on + strand at 4.468 kb on - strand, within GFF2207 at 4.473 kb on + strand, within GFF2207 at 4.473 kb on + strand, within GFF2207 at 4.474 kb on - strand, within GFF2207 at 4.474 kb on - strand, within GFF2207 at 4.480 kb on - strand, within GFF2207 at 4.513 kb on - strand, within GFF2207 at 4.531 kb on + strand, within GFF2207 at 4.531 kb on + strand, within GFF2207 at 4.532 kb on - strand, within GFF2207 at 4.568 kb on + strand at 4.570 kb on + strand at 4.578 kb on + strand at 4.644 kb on + strand at 4.645 kb on - strand at 4.645 kb on - strand at 4.677 kb on + strand at 4.726 kb on - strand at 4.825 kb on - strand at 4.845 kb on - strand at 4.861 kb on + strand at 4.871 kb on + strand at 5.042 kb on + strand, within GFF2208 at 5.052 kb on - strand, within GFF2208 at 5.053 kb on + strand, within GFF2208 at 5.101 kb on - strand at 5.103 kb on + strand at 5.215 kb on - strand at 5.250 kb on - strand at 5.265 kb on - strand at 5.266 kb on + strand at 5.266 kb on + strand at 5.266 kb on + strand at 5.266 kb on + strand at 5.266 kb on + strand at 5.267 kb on - strand at 5.285 kb on - strand at 5.323 kb on - strand at 5.323 kb on - strand at 5.323 kb on - strand at 5.325 kb on - strand at 5.383 kb on - strand at 5.390 kb on + strand at 5.390 kb on + strand at 5.432 kb on + strand at 5.432 kb on + strand at 5.465 kb on + strand at 5.502 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 1.875 MOI remove 3,508 + -0.4 3,544 + -0.2 3,692 + GFF2205 0.39 -0.1 3,692 + GFF2205 0.39 -0.3 3,693 - GFF2205 0.39 -0.3 3,693 - GFF2205 0.39 -0.8 3,799 + GFF2205 0.70 -0.4 3,800 - GFF2205 0.70 -0.1 3,850 - GFF2205 0.84 -0.3 3,948 + GFF2206 0.15 +0.1 4,042 - GFF2206 0.45 +0.1 4,154 - GFF2206 0.81 +0.6 4,213 + +0.2 4,213 + +0.3 4,213 + +0.1 4,251 + -0.4 4,255 + -0.5 4,256 - +0.0 4,356 + +0.3 4,356 + -0.6 4,390 + -0.2 4,468 - GFF2207 0.26 -0.6 4,473 + GFF2207 0.29 -0.2 4,473 + GFF2207 0.29 -2.0 4,474 - GFF2207 0.30 -0.0 4,474 - GFF2207 0.30 -0.8 4,480 - GFF2207 0.34 +0.2 4,513 - GFF2207 0.56 +0.6 4,531 + GFF2207 0.67 -0.6 4,531 + GFF2207 0.67 -0.4 4,532 - GFF2207 0.68 -0.3 4,568 + +0.1 4,570 + -0.3 4,578 + -0.5 4,644 + -0.3 4,645 - -0.6 4,645 - -0.2 4,677 + -0.2 4,726 - +0.3 4,825 - +0.4 4,845 - -0.3 4,861 + -0.3 4,871 + -0.0 5,042 + GFF2208 0.73 -0.7 5,052 - GFF2208 0.77 -0.1 5,053 + GFF2208 0.77 +0.8 5,101 - +0.3 5,103 + -0.1 5,215 - -1.6 5,250 - -0.4 5,265 - -0.2 5,266 + +0.1 5,266 + -0.3 5,266 + -0.6 5,266 + +0.2 5,266 + +0.5 5,267 - +0.6 5,285 - +0.3 5,323 - -0.4 5,323 - -0.1 5,323 - +0.1 5,325 - -0.4 5,383 - -0.1 5,390 + +0.4 5,390 + -0.2 5,432 + -0.2 5,432 + +0.3 5,465 + +0.4 5,502 - +0.0
Or see this region's nucleotide sequence