Experiment: LB_plus_SM_buffer with Ffm_phage 1.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2169 and GFF2170 are separated by 35 nucleotides GFF2170 and GFF2171 are separated by 234 nucleotides GFF2171 and GFF2172 are separated by 87 nucleotides
GFF2169 - Phosphoribosylformylglycinamidine cyclo-ligase (EC 6.3.3.1), at 17,274 to 18,311
GFF2169
GFF2170 - Phosphoribosylformylglycinamidine cyclo-ligase (EC 6.3.3.1), at 18,347 to 18,490
GFF2170
GFF2171 - Uracil phosphoribosyltransferase (EC 2.4.2.9), at 18,725 to 19,351
GFF2171
GFF2172 - Uracil permease, at 19,439 to 20,728
GFF2172
Position (kb) 18
19
20 Strain fitness (log2 ratio) -1
0
1 at 17.866 kb on + strand, within GFF2169 at 17.867 kb on - strand, within GFF2169 at 17.867 kb on - strand, within GFF2169 at 17.989 kb on + strand, within GFF2169 at 18.111 kb on + strand, within GFF2169 at 18.226 kb on - strand at 18.226 kb on - strand at 18.291 kb on - strand at 18.639 kb on + strand at 18.640 kb on - strand at 18.799 kb on + strand, within GFF2171 at 18.828 kb on + strand, within GFF2171 at 19.029 kb on - strand, within GFF2171 at 19.061 kb on + strand, within GFF2171 at 19.177 kb on + strand, within GFF2171 at 19.186 kb on + strand, within GFF2171 at 19.260 kb on - strand, within GFF2171 at 19.299 kb on - strand at 19.499 kb on - strand at 19.558 kb on + strand at 19.697 kb on + strand, within GFF2172 at 20.016 kb on - strand, within GFF2172 at 20.300 kb on + strand, within GFF2172 at 20.301 kb on - strand, within GFF2172 at 20.301 kb on - strand, within GFF2172
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 1.875 MOI remove 17,866 + GFF2169 0.57 -0.3 17,867 - GFF2169 0.57 -0.6 17,867 - GFF2169 0.57 +0.3 17,989 + GFF2169 0.69 -0.6 18,111 + GFF2169 0.81 -0.1 18,226 - -0.6 18,226 - +0.3 18,291 - -0.1 18,639 + -0.2 18,640 - -0.1 18,799 + GFF2171 0.12 +0.7 18,828 + GFF2171 0.16 +1.0 19,029 - GFF2171 0.48 +1.2 19,061 + GFF2171 0.54 +0.4 19,177 + GFF2171 0.72 +0.6 19,186 + GFF2171 0.74 +0.4 19,260 - GFF2171 0.85 -0.2 19,299 - +0.7 19,499 - +0.4 19,558 + -1.1 19,697 + GFF2172 0.20 +0.1 20,016 - GFF2172 0.45 +0.4 20,300 + GFF2172 0.67 +0.1 20,301 - GFF2172 0.67 -0.7 20,301 - GFF2172 0.67 +0.5
Or see this region's nucleotide sequence