Experiment: LB_plus_SM_buffer with Ffm_phage 1.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2099 and GFF2100 are separated by 115 nucleotides GFF2100 and GFF2101 are separated by 318 nucleotides GFF2101 and GFF2102 are separated by 61 nucleotides
GFF2099 - hypothetical tRNA/rRNA methyltransferase yfiF [EC:2.1.1.-], at 8,234 to 9,271
GFF2099
GFF2100 - Uracil-DNA glycosylase, family 1, at 9,387 to 10,076
GFF2100
GFF2101 - Pyruvate formate-lyase (EC 2.3.1.54), at 10,395 to 10,778
GFF2101
GFF2102 - Transporter, LysE family, at 10,840 to 11,427
GFF2102
Position (kb)
9
10
11 Strain fitness (log2 ratio)
-2
-1
0
1 at 8.420 kb on - strand, within GFF2099 at 8.420 kb on - strand, within GFF2099 at 8.515 kb on + strand, within GFF2099 at 8.515 kb on + strand, within GFF2099 at 8.782 kb on + strand, within GFF2099 at 8.803 kb on - strand, within GFF2099 at 8.882 kb on - strand, within GFF2099 at 8.917 kb on - strand, within GFF2099 at 9.120 kb on + strand, within GFF2099 at 9.137 kb on + strand, within GFF2099 at 9.314 kb on + strand at 9.315 kb on - strand at 9.533 kb on + strand, within GFF2100 at 9.533 kb on + strand, within GFF2100 at 9.799 kb on + strand, within GFF2100 at 9.800 kb on - strand, within GFF2100 at 9.804 kb on - strand, within GFF2100 at 9.804 kb on - strand, within GFF2100 at 9.804 kb on - strand, within GFF2100 at 9.804 kb on - strand, within GFF2100 at 10.010 kb on + strand at 10.106 kb on + strand at 10.309 kb on + strand at 10.309 kb on + strand at 10.309 kb on + strand at 10.309 kb on + strand at 10.310 kb on - strand at 10.311 kb on + strand at 10.312 kb on - strand at 10.372 kb on - strand at 10.406 kb on + strand at 10.415 kb on + strand at 10.418 kb on + strand at 10.890 kb on + strand at 10.894 kb on + strand at 10.987 kb on + strand, within GFF2102 at 10.987 kb on + strand, within GFF2102 at 10.987 kb on + strand, within GFF2102 at 10.988 kb on - strand, within GFF2102 at 10.990 kb on - strand, within GFF2102 at 11.040 kb on + strand, within GFF2102 at 11.040 kb on + strand, within GFF2102
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 1.875 MOI remove 8,420 - GFF2099 0.18 +0.6 8,420 - GFF2099 0.18 -0.1 8,515 + GFF2099 0.27 -0.5 8,515 + GFF2099 0.27 +0.2 8,782 + GFF2099 0.53 +0.1 8,803 - GFF2099 0.55 -0.3 8,882 - GFF2099 0.62 -0.1 8,917 - GFF2099 0.66 -0.1 9,120 + GFF2099 0.85 +0.1 9,137 + GFF2099 0.87 -0.8 9,314 + +0.2 9,315 - +0.3 9,533 + GFF2100 0.21 +0.3 9,533 + GFF2100 0.21 +1.3 9,799 + GFF2100 0.60 +0.4 9,800 - GFF2100 0.60 +0.3 9,804 - GFF2100 0.60 +0.1 9,804 - GFF2100 0.60 -2.0 9,804 - GFF2100 0.60 +0.2 9,804 - GFF2100 0.60 -0.0 10,010 + -0.4 10,106 + -0.3 10,309 + -0.2 10,309 + +0.5 10,309 + +0.3 10,309 + +0.1 10,310 - -0.5 10,311 + +0.1 10,312 - +0.0 10,372 - -0.0 10,406 + -0.7 10,415 + +0.0 10,418 + +1.3 10,890 + +0.2 10,894 + +0.0 10,987 + GFF2102 0.25 +0.5 10,987 + GFF2102 0.25 -0.1 10,987 + GFF2102 0.25 +0.0 10,988 - GFF2102 0.25 +0.2 10,990 - GFF2102 0.26 +0.0 11,040 + GFF2102 0.34 -0.2 11,040 + GFF2102 0.34 +0.5
Or see this region's nucleotide sequence