Experiment: LB_plus_SM_buffer with Ffm_phage 1.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2069 and GFF2070 are separated by 47 nucleotides GFF2070 and GFF2071 are separated by 12 nucleotides GFF2071 and GFF2072 are separated by 9 nucleotides GFF2072 and GFF2073 are separated by 18 nucleotides
GFF2069 - Ethanolamine operon regulatory protein, at 32,079 to 33,131
GFF2069
GFF2070 - Ethanolamine utilization polyhedral-body-like protein EutK, at 33,179 to 33,673
GFF2070
GFF2071 - Ethanolamine utilization polyhedral-body-like protein EutL, at 33,686 to 34,345
GFF2071
GFF2072 - Ethanolamine ammonia-lyase light chain (EC 4.3.1.7), at 34,355 to 35,251
GFF2072
GFF2073 - Ethanolamine ammonia-lyase heavy chain (EC 4.3.1.7), at 35,270 to 36,631
GFF2073
Position (kb) 33
34
35 Strain fitness (log2 ratio) -1
0
1 at 32.688 kb on - strand, within GFF2069 at 32.722 kb on + strand, within GFF2069 at 33.095 kb on - strand at 33.116 kb on + strand at 33.607 kb on + strand, within GFF2070 at 33.707 kb on - strand at 33.733 kb on + strand at 33.819 kb on + strand, within GFF2071 at 33.942 kb on + strand, within GFF2071 at 33.943 kb on - strand, within GFF2071 at 34.153 kb on - strand, within GFF2071 at 34.199 kb on - strand, within GFF2071 at 34.200 kb on + strand, within GFF2071 at 34.241 kb on - strand, within GFF2071 at 34.241 kb on - strand, within GFF2071 at 34.245 kb on - strand, within GFF2071 at 34.245 kb on - strand, within GFF2071 at 34.245 kb on - strand, within GFF2071 at 34.246 kb on + strand, within GFF2071 at 34.246 kb on + strand, within GFF2071 at 34.247 kb on - strand, within GFF2071 at 34.247 kb on - strand, within GFF2071 at 34.247 kb on - strand, within GFF2071 at 34.262 kb on - strand, within GFF2071 at 34.710 kb on + strand, within GFF2072 at 34.710 kb on + strand, within GFF2072 at 34.711 kb on - strand, within GFF2072 at 34.754 kb on + strand, within GFF2072 at 34.754 kb on + strand, within GFF2072 at 34.846 kb on - strand, within GFF2072 at 35.222 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 1.875 MOI remove 32,688 - GFF2069 0.58 +0.0 32,722 + GFF2069 0.61 +0.2 33,095 - +0.8 33,116 + +0.0 33,607 + GFF2070 0.86 +0.2 33,707 - +0.9 33,733 + +0.3 33,819 + GFF2071 0.20 +0.3 33,942 + GFF2071 0.39 +0.9 33,943 - GFF2071 0.39 +0.8 34,153 - GFF2071 0.71 -0.2 34,199 - GFF2071 0.78 +0.2 34,200 + GFF2071 0.78 -0.2 34,241 - GFF2071 0.84 +0.1 34,241 - GFF2071 0.84 +0.2 34,245 - GFF2071 0.85 +0.8 34,245 - GFF2071 0.85 -0.1 34,245 - GFF2071 0.85 -0.1 34,246 + GFF2071 0.85 +0.1 34,246 + GFF2071 0.85 +0.2 34,247 - GFF2071 0.85 +0.3 34,247 - GFF2071 0.85 +0.1 34,247 - GFF2071 0.85 +0.5 34,262 - GFF2071 0.87 +1.1 34,710 + GFF2072 0.40 -0.8 34,710 + GFF2072 0.40 +0.3 34,711 - GFF2072 0.40 +0.4 34,754 + GFF2072 0.44 -0.1 34,754 + GFF2072 0.44 -0.2 34,846 - GFF2072 0.55 -1.2 35,222 + +1.3
Or see this region's nucleotide sequence