Experiment: LB_plus_SM_buffer with Ffm_phage 1.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1900 and GFF1901 are separated by 76 nucleotides GFF1901 and GFF1902 are separated by 217 nucleotides
GFF1900 - Acetate kinase (EC 2.7.2.1), at 94,329 to 95,531
GFF1900
GFF1901 - Phosphate acetyltransferase (EC 2.3.1.8), at 95,608 to 97,752
GFF1901
GFF1902 - Putative S-transferase, at 97,970 to 99,490
GFF1902
Position (kb) 95
96
97
98 Strain fitness (log2 ratio) -1
0
1 at 94.625 kb on + strand, within GFF1900 at 94.812 kb on + strand, within GFF1900 at 94.813 kb on - strand, within GFF1900 at 94.841 kb on + strand, within GFF1900 at 94.842 kb on - strand, within GFF1900 at 94.890 kb on + strand, within GFF1900 at 94.891 kb on - strand, within GFF1900 at 94.946 kb on + strand, within GFF1900 at 95.190 kb on + strand, within GFF1900 at 95.190 kb on + strand, within GFF1900 at 95.205 kb on + strand, within GFF1900 at 95.265 kb on + strand, within GFF1900 at 95.277 kb on + strand, within GFF1900 at 95.617 kb on - strand at 95.619 kb on + strand at 95.622 kb on + strand at 95.623 kb on - strand at 95.946 kb on + strand, within GFF1901 at 96.058 kb on - strand, within GFF1901 at 96.115 kb on - strand, within GFF1901 at 96.390 kb on + strand, within GFF1901 at 96.434 kb on + strand, within GFF1901 at 96.498 kb on - strand, within GFF1901 at 96.824 kb on + strand, within GFF1901 at 96.852 kb on + strand, within GFF1901 at 97.204 kb on - strand, within GFF1901 at 97.706 kb on - strand at 97.756 kb on + strand at 97.764 kb on - strand at 97.799 kb on + strand at 97.862 kb on - strand at 97.937 kb on - strand at 98.095 kb on + strand at 98.308 kb on + strand, within GFF1902 at 98.314 kb on + strand, within GFF1902 at 98.314 kb on + strand, within GFF1902 at 98.314 kb on + strand, within GFF1902 at 98.387 kb on - strand, within GFF1902 at 98.430 kb on + strand, within GFF1902 at 98.497 kb on + strand, within GFF1902 at 98.497 kb on + strand, within GFF1902
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 1.875 MOI remove 94,625 + GFF1900 0.25 -0.5 94,812 + GFF1900 0.40 -0.9 94,813 - GFF1900 0.40 +0.3 94,841 + GFF1900 0.43 +0.0 94,842 - GFF1900 0.43 -1.0 94,890 + GFF1900 0.47 -1.3 94,891 - GFF1900 0.47 +0.8 94,946 + GFF1900 0.51 -1.4 95,190 + GFF1900 0.72 +0.2 95,190 + GFF1900 0.72 -0.8 95,205 + GFF1900 0.73 +0.2 95,265 + GFF1900 0.78 -0.3 95,277 + GFF1900 0.79 +0.8 95,617 - -0.4 95,619 + -0.5 95,622 + -0.5 95,623 - -0.3 95,946 + GFF1901 0.16 -1.1 96,058 - GFF1901 0.21 -0.6 96,115 - GFF1901 0.24 -1.2 96,390 + GFF1901 0.36 -0.2 96,434 + GFF1901 0.39 -0.4 96,498 - GFF1901 0.41 +0.2 96,824 + GFF1901 0.57 -0.8 96,852 + GFF1901 0.58 -0.5 97,204 - GFF1901 0.74 -0.4 97,706 - +0.3 97,756 + -0.4 97,764 - +0.1 97,799 + +0.2 97,862 - +0.1 97,937 - +0.4 98,095 + +0.4 98,308 + GFF1902 0.22 -0.5 98,314 + GFF1902 0.23 +0.1 98,314 + GFF1902 0.23 -0.5 98,314 + GFF1902 0.23 +0.1 98,387 - GFF1902 0.27 +0.1 98,430 + GFF1902 0.30 -0.3 98,497 + GFF1902 0.35 +1.4 98,497 + GFF1902 0.35 -0.2
Or see this region's nucleotide sequence