Experiment: LB_plus_SM_buffer with Ffm_phage 1.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1785 and GFF1786 are separated by 127 nucleotides GFF1786 and GFF1787 are separated by 119 nucleotides
GFF1785 - Mlc, transcriptional repressor of MalT (the transcriptional activator of maltose regulon) and manXYZ operon, at 7,462 to 8,682
GFF1785
GFF1786 - LysR family transcriptional regulator YnfL, at 8,810 to 9,709
GFF1786
GFF1787 - Permeases of the major facilitator superfamily, at 9,829 to 11,082
GFF1787
Position (kb)
8
9
10 Strain fitness (log2 ratio)
-1
0
1 at 7.902 kb on + strand, within GFF1785 at 7.965 kb on + strand, within GFF1785 at 7.966 kb on - strand, within GFF1785 at 8.017 kb on - strand, within GFF1785 at 8.097 kb on + strand, within GFF1785 at 8.097 kb on + strand, within GFF1785 at 8.098 kb on - strand, within GFF1785 at 8.174 kb on + strand, within GFF1785 at 8.419 kb on + strand, within GFF1785 at 8.644 kb on - strand at 8.671 kb on - strand at 8.727 kb on - strand at 8.807 kb on + strand at 8.834 kb on + strand at 8.889 kb on - strand at 8.954 kb on + strand, within GFF1786 at 9.062 kb on - strand, within GFF1786 at 9.257 kb on - strand, within GFF1786 at 9.337 kb on + strand, within GFF1786 at 9.540 kb on - strand, within GFF1786 at 9.626 kb on - strand at 9.696 kb on - strand at 9.774 kb on + strand at 9.812 kb on + strand at 9.813 kb on - strand at 9.813 kb on - strand at 9.813 kb on - strand at 9.837 kb on + strand at 9.843 kb on + strand at 9.907 kb on - strand at 10.000 kb on - strand, within GFF1787 at 10.001 kb on + strand, within GFF1787 at 10.002 kb on - strand, within GFF1787 at 10.002 kb on - strand, within GFF1787 at 10.206 kb on + strand, within GFF1787 at 10.271 kb on - strand, within GFF1787 at 10.322 kb on - strand, within GFF1787
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 1.875 MOI remove 7,902 + GFF1785 0.36 +0.1 7,965 + GFF1785 0.41 -0.1 7,966 - GFF1785 0.41 +0.6 8,017 - GFF1785 0.45 +1.2 8,097 + GFF1785 0.52 +0.6 8,097 + GFF1785 0.52 +0.2 8,098 - GFF1785 0.52 +0.8 8,174 + GFF1785 0.58 +1.3 8,419 + GFF1785 0.78 +0.1 8,644 - -0.1 8,671 - +0.1 8,727 - +0.3 8,807 + -0.6 8,834 + +0.2 8,889 - -0.2 8,954 + GFF1786 0.16 -0.4 9,062 - GFF1786 0.28 -0.5 9,257 - GFF1786 0.50 +0.0 9,337 + GFF1786 0.59 +0.3 9,540 - GFF1786 0.81 -0.9 9,626 - +0.3 9,696 - -0.2 9,774 + +0.6 9,812 + -1.1 9,813 - -0.5 9,813 - -0.9 9,813 - +0.0 9,837 + -0.2 9,843 + +0.1 9,907 - -0.0 10,000 - GFF1787 0.14 -0.2 10,001 + GFF1787 0.14 -0.9 10,002 - GFF1787 0.14 -0.5 10,002 - GFF1787 0.14 +0.0 10,206 + GFF1787 0.30 +0.5 10,271 - GFF1787 0.35 -0.3 10,322 - GFF1787 0.39 -0.8
Or see this region's nucleotide sequence