Experiment: LB_plus_SM_buffer with Ffm_phage 1.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1407 and GFF1408 are separated by 180 nucleotides GFF1408 and GFF1409 are separated by 13 nucleotides GFF1409 and GFF1410 are separated by 98 nucleotides
GFF1407 - Maltose operon periplasmic protein MalM, at 53,731 to 54,648
GFF1407
GFF1408 - Chorismate--pyruvate lyase (EC 4.1.3.40), at 54,829 to 55,326
GFF1408
GFF1409 - 4-hydroxybenzoate polyprenyltransferase (EC 2.5.1.39), at 55,340 to 56,212
GFF1409
GFF1410 - Glycerol-3-phosphate acyltransferase (EC 2.3.1.15), at 56,311 to 58,731
GFF1410
Position (kb) 54
55
56 Strain fitness (log2 ratio) -2
-1
0
1 at 53.958 kb on + strand, within GFF1407 at 53.977 kb on + strand, within GFF1407 at 53.978 kb on - strand, within GFF1407 at 54.217 kb on - strand, within GFF1407 at 54.246 kb on + strand, within GFF1407 at 54.246 kb on + strand, within GFF1407 at 54.271 kb on + strand, within GFF1407 at 54.289 kb on - strand, within GFF1407 at 54.516 kb on + strand, within GFF1407 at 54.650 kb on + strand at 54.651 kb on - strand at 54.755 kb on + strand at 54.793 kb on + strand at 54.804 kb on + strand at 54.845 kb on + strand at 54.869 kb on - strand at 55.028 kb on + strand, within GFF1408 at 55.048 kb on + strand, within GFF1408 at 55.056 kb on - strand, within GFF1408 at 55.065 kb on - strand, within GFF1408 at 55.184 kb on + strand, within GFF1408 at 55.184 kb on + strand, within GFF1408 at 55.185 kb on - strand, within GFF1408 at 55.185 kb on - strand, within GFF1408 at 55.185 kb on - strand, within GFF1408 at 55.185 kb on - strand, within GFF1408 at 55.188 kb on + strand, within GFF1408 at 55.189 kb on - strand, within GFF1408 at 55.189 kb on - strand, within GFF1408 at 55.322 kb on - strand at 56.198 kb on + strand at 56.263 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 1.875 MOI remove 53,958 + GFF1407 0.25 +0.1 53,977 + GFF1407 0.27 -0.2 53,978 - GFF1407 0.27 +0.4 54,217 - GFF1407 0.53 +0.2 54,246 + GFF1407 0.56 +0.0 54,246 + GFF1407 0.56 +0.1 54,271 + GFF1407 0.59 -0.0 54,289 - GFF1407 0.61 +0.4 54,516 + GFF1407 0.86 -0.3 54,650 + +0.0 54,651 - +0.7 54,755 + +0.4 54,793 + +0.3 54,804 + +0.1 54,845 + +0.2 54,869 - -0.9 55,028 + GFF1408 0.40 +0.2 55,048 + GFF1408 0.44 -0.4 55,056 - GFF1408 0.46 +0.0 55,065 - GFF1408 0.47 -0.7 55,184 + GFF1408 0.71 -0.2 55,184 + GFF1408 0.71 -0.3 55,185 - GFF1408 0.71 -1.3 55,185 - GFF1408 0.71 -0.1 55,185 - GFF1408 0.71 -0.5 55,185 - GFF1408 0.71 -0.6 55,188 + GFF1408 0.72 -1.2 55,189 - GFF1408 0.72 -0.1 55,189 - GFF1408 0.72 -1.8 55,322 - -1.3 56,198 + +0.4 56,263 + +0.1
Or see this region's nucleotide sequence