Experiment: LB_plus_SM_buffer with Ffm_phage 1.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1362 and GFF1363 overlap by 4 nucleotides GFF1363 and GFF1364 are separated by 97 nucleotides GFF1364 and GFF1365 are separated by 117 nucleotides GFF1365 and GFF1366 overlap by 12 nucleotides
GFF1362 - Ribosomal large subunit pseudouridine synthase F (EC 4.2.1.70), at 15,607 to 16,476
GFF1362
GFF1363 - hypothetical protein, at 16,473 to 16,745
GFF1363
GFF1364 - Sodium-dependent transporter, at 16,843 to 17,784
GFF1364
GFF1365 - FIG01046261: hypothetical protein, at 17,902 to 18,057
GFF1365
GFF1366 - putative cytoplasmic protein, at 18,046 to 18,774
GFF1366
Position (kb)
16
17
18 Strain fitness (log2 ratio)
-1
0
1 at 15.948 kb on - strand, within GFF1362 at 16.107 kb on + strand, within GFF1362 at 16.180 kb on - strand, within GFF1362 at 16.537 kb on + strand, within GFF1363 at 16.601 kb on + strand, within GFF1363 at 16.610 kb on - strand, within GFF1363 at 17.107 kb on - strand, within GFF1364 at 17.151 kb on + strand, within GFF1364 at 17.152 kb on - strand, within GFF1364 at 17.455 kb on + strand, within GFF1364 at 17.456 kb on - strand, within GFF1364 at 17.563 kb on + strand, within GFF1364 at 17.661 kb on + strand, within GFF1364 at 17.662 kb on - strand, within GFF1364 at 17.720 kb on + strand at 17.723 kb on + strand at 17.802 kb on + strand at 17.845 kb on + strand at 17.846 kb on - strand at 17.846 kb on - strand at 17.846 kb on - strand at 17.849 kb on + strand at 17.850 kb on - strand at 17.955 kb on - strand, within GFF1365 at 17.982 kb on - strand, within GFF1365 at 18.066 kb on - strand at 18.102 kb on + strand at 18.107 kb on + strand at 18.261 kb on + strand, within GFF1366 at 18.549 kb on + strand, within GFF1366 at 18.708 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 1.875 MOI remove 15,948 - GFF1362 0.39 -0.1 16,107 + GFF1362 0.57 -0.1 16,180 - GFF1362 0.66 -0.2 16,537 + GFF1363 0.23 -0.4 16,601 + GFF1363 0.47 -0.2 16,610 - GFF1363 0.50 +1.0 17,107 - GFF1364 0.28 +0.3 17,151 + GFF1364 0.33 +0.4 17,152 - GFF1364 0.33 +0.1 17,455 + GFF1364 0.65 -0.1 17,456 - GFF1364 0.65 +0.3 17,563 + GFF1364 0.76 +0.8 17,661 + GFF1364 0.87 +0.9 17,662 - GFF1364 0.87 -0.1 17,720 + +0.5 17,723 + +0.1 17,802 + -0.2 17,845 + -0.2 17,846 - -0.2 17,846 - -0.4 17,846 - -0.4 17,849 + +0.4 17,850 - +0.1 17,955 - GFF1365 0.34 +0.3 17,982 - GFF1365 0.51 +0.2 18,066 - -0.3 18,102 + -0.0 18,107 + +0.2 18,261 + GFF1366 0.29 +0.2 18,549 + GFF1366 0.69 +0.6 18,708 + +0.2
Or see this region's nucleotide sequence