Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF1351

Experiment: LB_plus_SM_buffer with Ffm_phage 1.875 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1350 and GFF1351 are separated by 265 nucleotidesGFF1351 and GFF1352 are separated by 31 nucleotides GFF1350 - Homoserine O-succinyltransferase (EC 2.3.1.46), at 969 to 1,898 GFF1350 GFF1351 - Malate synthase (EC 2.3.3.9), at 2,164 to 3,768 GFF1351 GFF1352 - Isocitrate lyase (EC 4.1.3.1), at 3,800 to 5,104 GFF1352 Position (kb) 2 3 4Strain fitness (log2 ratio) -2 -1 0 1at 1.234 kb on - strand, within GFF1350at 1.374 kb on - strand, within GFF1350at 1.419 kb on - strand, within GFF1350at 1.470 kb on + strand, within GFF1350at 1.470 kb on + strand, within GFF1350at 1.549 kb on - strand, within GFF1350at 1.647 kb on - strand, within GFF1350at 1.652 kb on + strand, within GFF1350at 1.652 kb on + strand, within GFF1350at 1.652 kb on + strand, within GFF1350at 1.653 kb on - strand, within GFF1350at 1.680 kb on + strand, within GFF1350at 1.755 kb on + strand, within GFF1350at 1.824 kb on - strandat 1.842 kb on + strandat 2.139 kb on - strandat 2.199 kb on - strandat 2.466 kb on + strand, within GFF1351at 2.467 kb on - strand, within GFF1351at 2.479 kb on - strand, within GFF1351at 2.553 kb on + strand, within GFF1351at 2.602 kb on + strand, within GFF1351at 2.602 kb on + strand, within GFF1351at 2.603 kb on - strand, within GFF1351at 2.603 kb on - strand, within GFF1351at 2.603 kb on - strand, within GFF1351at 2.666 kb on + strand, within GFF1351at 2.667 kb on - strand, within GFF1351at 2.676 kb on - strand, within GFF1351at 2.697 kb on + strand, within GFF1351at 2.698 kb on - strand, within GFF1351at 2.762 kb on - strand, within GFF1351at 2.762 kb on - strand, within GFF1351at 2.784 kb on + strand, within GFF1351at 2.800 kb on + strand, within GFF1351at 3.633 kb on - strandat 3.767 kb on - strandat 3.778 kb on + strandat 3.832 kb on - strandat 4.064 kb on + strand, within GFF1352at 4.065 kb on - strand, within GFF1352at 4.116 kb on - strand, within GFF1352at 4.247 kb on + strand, within GFF1352at 4.555 kb on + strand, within GFF1352at 4.586 kb on + strand, within GFF1352at 4.673 kb on - strand, within GFF1352at 4.673 kb on - strand, within GFF1352at 4.673 kb on - strand, within GFF1352at 4.747 kb on + strand, within GFF1352

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 1.875 MOI
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1,234 - GFF1350 0.28 +0.3
1,374 - GFF1350 0.44 -0.8
1,419 - GFF1350 0.48 -0.1
1,470 + GFF1350 0.54 +0.3
1,470 + GFF1350 0.54 -0.1
1,549 - GFF1350 0.62 -0.1
1,647 - GFF1350 0.73 -0.7
1,652 + GFF1350 0.73 -0.3
1,652 + GFF1350 0.73 -0.0
1,652 + GFF1350 0.73 -2.3
1,653 - GFF1350 0.74 -0.1
1,680 + GFF1350 0.76 -0.6
1,755 + GFF1350 0.85 +0.3
1,824 - -1.5
1,842 + +0.2
2,139 - -0.3
2,199 - -0.2
2,466 + GFF1351 0.19 +0.1
2,467 - GFF1351 0.19 -0.2
2,479 - GFF1351 0.20 -0.2
2,553 + GFF1351 0.24 -0.2
2,602 + GFF1351 0.27 -0.1
2,602 + GFF1351 0.27 -0.0
2,603 - GFF1351 0.27 -0.1
2,603 - GFF1351 0.27 -0.5
2,603 - GFF1351 0.27 +0.4
2,666 + GFF1351 0.31 +0.1
2,667 - GFF1351 0.31 +0.1
2,676 - GFF1351 0.32 -0.6
2,697 + GFF1351 0.33 -0.3
2,698 - GFF1351 0.33 +0.2
2,762 - GFF1351 0.37 +0.3
2,762 - GFF1351 0.37 +0.2
2,784 + GFF1351 0.39 -0.6
2,800 + GFF1351 0.40 +0.5
3,633 - +0.3
3,767 - -0.0
3,778 + +0.2
3,832 - +0.1
4,064 + GFF1352 0.20 +0.2
4,065 - GFF1352 0.20 +0.1
4,116 - GFF1352 0.24 +0.4
4,247 + GFF1352 0.34 +0.5
4,555 + GFF1352 0.58 -0.4
4,586 + GFF1352 0.60 +0.1
4,673 - GFF1352 0.67 +0.3
4,673 - GFF1352 0.67 -1.0
4,673 - GFF1352 0.67 +0.9
4,747 + GFF1352 0.73 -0.8

Or see this region's nucleotide sequence