Experiment: LB_plus_SM_buffer with Ffm_phage 1.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1263 and GFF1264 overlap by 1 nucleotides GFF1264 and GFF1265 are separated by 55 nucleotides GFF1265 and GFF1266 are separated by 71 nucleotides GFF1266 and GFF1267 overlap by 20 nucleotides GFF1267 and GFF1268 are separated by 290 nucleotides GFF1268 and GFF1269 are separated by 66 nucleotides
GFF1263 - Adenine-specific methyltransferase (EC 2.1.1.72), at 89,703 to 90,383
GFF1263
GFF1264 - putative cytoplasmic protein, at 90,383 to 90,604
GFF1264
GFF1265 - FIG01046678: hypothetical protein, at 90,660 to 91,073
GFF1265
GFF1266 - FIG00638373: hypothetical protein, at 91,145 to 91,909
GFF1266
GFF1267 - FIG01047435: hypothetical protein, at 91,890 to 92,114
GFF1267
GFF1268 - Putative antirestriction protein, at 92,405 to 92,839
GFF1268
GFF1269 - FIG074102: hypothetical protein, at 92,906 to 93,340
GFF1269
Position (kb)
91
92 Strain fitness (log2 ratio)
-1
0
1 at 90.267 kb on - strand, within GFF1263 at 90.563 kb on + strand, within GFF1264 at 90.564 kb on - strand, within GFF1264 at 90.666 kb on + strand at 90.666 kb on + strand at 90.666 kb on + strand at 90.666 kb on + strand at 90.667 kb on - strand at 90.758 kb on + strand, within GFF1265 at 90.758 kb on + strand, within GFF1265 at 90.997 kb on + strand, within GFF1265 at 90.997 kb on + strand, within GFF1265 at 90.997 kb on + strand, within GFF1265 at 90.997 kb on + strand, within GFF1265 at 90.998 kb on - strand, within GFF1265 at 90.998 kb on - strand, within GFF1265 at 91.105 kb on + strand at 91.162 kb on + strand at 91.171 kb on - strand at 91.186 kb on + strand at 91.331 kb on - strand, within GFF1266 at 91.331 kb on - strand, within GFF1266 at 91.562 kb on - strand, within GFF1266 at 91.652 kb on + strand, within GFF1266 at 91.652 kb on + strand, within GFF1266 at 91.663 kb on + strand, within GFF1266 at 91.663 kb on + strand, within GFF1266 at 91.722 kb on + strand, within GFF1266 at 91.911 kb on + strand at 91.912 kb on - strand at 91.933 kb on - strand, within GFF1267 at 92.161 kb on - strand at 92.386 kb on - strand at 92.404 kb on + strand at 92.405 kb on - strand at 92.405 kb on - strand at 92.405 kb on - strand at 92.437 kb on + strand at 92.440 kb on + strand at 92.512 kb on - strand, within GFF1268 at 92.512 kb on - strand, within GFF1268 at 92.517 kb on + strand, within GFF1268 at 92.517 kb on + strand, within GFF1268 at 92.517 kb on + strand, within GFF1268 at 92.517 kb on + strand, within GFF1268 at 92.517 kb on + strand, within GFF1268 at 92.517 kb on + strand, within GFF1268 at 92.518 kb on - strand, within GFF1268 at 92.518 kb on - strand, within GFF1268 at 92.518 kb on - strand, within GFF1268 at 92.518 kb on - strand, within GFF1268 at 92.518 kb on - strand, within GFF1268 at 92.520 kb on - strand, within GFF1268 at 92.520 kb on - strand, within GFF1268 at 92.520 kb on - strand, within GFF1268 at 92.549 kb on + strand, within GFF1268 at 92.549 kb on + strand, within GFF1268 at 92.759 kb on + strand, within GFF1268 at 92.759 kb on + strand, within GFF1268 at 92.759 kb on + strand, within GFF1268 at 92.759 kb on + strand, within GFF1268 at 92.759 kb on + strand, within GFF1268 at 92.761 kb on + strand, within GFF1268 at 92.761 kb on + strand, within GFF1268 at 92.762 kb on - strand, within GFF1268 at 92.762 kb on - strand, within GFF1268 at 92.794 kb on + strand, within GFF1268 at 92.795 kb on - strand, within GFF1268 at 92.795 kb on - strand, within GFF1268 at 92.819 kb on - strand at 92.872 kb on + strand at 92.906 kb on - strand at 92.906 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 1.875 MOI remove 90,267 - GFF1263 0.83 -0.2 90,563 + GFF1264 0.81 +0.1 90,564 - GFF1264 0.82 -0.3 90,666 + -0.5 90,666 + -0.2 90,666 + +0.4 90,666 + +0.8 90,667 - -0.9 90,758 + GFF1265 0.24 -0.4 90,758 + GFF1265 0.24 -0.1 90,997 + GFF1265 0.81 +0.4 90,997 + GFF1265 0.81 +0.2 90,997 + GFF1265 0.81 -0.0 90,997 + GFF1265 0.81 -0.0 90,998 - GFF1265 0.82 -0.2 90,998 - GFF1265 0.82 -0.2 91,105 + -0.2 91,162 + +0.3 91,171 - -1.0 91,186 + +0.5 91,331 - GFF1266 0.24 -0.0 91,331 - GFF1266 0.24 +0.9 91,562 - GFF1266 0.55 -0.3 91,652 + GFF1266 0.66 +0.4 91,652 + GFF1266 0.66 +0.4 91,663 + GFF1266 0.68 +0.2 91,663 + GFF1266 0.68 -0.4 91,722 + GFF1266 0.75 -0.0 91,911 + +0.3 91,912 - +0.3 91,933 - GFF1267 0.19 +0.3 92,161 - +0.2 92,386 - -0.1 92,404 + -0.4 92,405 - +0.4 92,405 - +0.3 92,405 - -0.4 92,437 + +0.1 92,440 + -0.5 92,512 - GFF1268 0.25 +0.5 92,512 - GFF1268 0.25 +0.3 92,517 + GFF1268 0.26 +0.4 92,517 + GFF1268 0.26 -0.1 92,517 + GFF1268 0.26 +0.2 92,517 + GFF1268 0.26 -0.2 92,517 + GFF1268 0.26 -0.3 92,517 + GFF1268 0.26 -0.0 92,518 - GFF1268 0.26 +0.4 92,518 - GFF1268 0.26 -0.3 92,518 - GFF1268 0.26 +0.5 92,518 - GFF1268 0.26 -0.3 92,518 - GFF1268 0.26 -0.0 92,520 - GFF1268 0.26 -0.3 92,520 - GFF1268 0.26 -0.1 92,520 - GFF1268 0.26 -0.6 92,549 + GFF1268 0.33 +0.5 92,549 + GFF1268 0.33 +0.1 92,759 + GFF1268 0.81 -1.1 92,759 + GFF1268 0.81 +0.5 92,759 + GFF1268 0.81 -1.3 92,759 + GFF1268 0.81 -0.5 92,759 + GFF1268 0.81 -1.2 92,761 + GFF1268 0.82 +0.8 92,761 + GFF1268 0.82 -1.1 92,762 - GFF1268 0.82 +0.8 92,762 - GFF1268 0.82 +0.0 92,794 + GFF1268 0.89 +0.4 92,795 - GFF1268 0.90 +0.7 92,795 - GFF1268 0.90 +0.6 92,819 - -0.0 92,872 + +0.0 92,906 - +0.2 92,906 - +0.2
Or see this region's nucleotide sequence