Experiment: LB_plus_SM_buffer with Ffm_phage 1.875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1017 and GFF1018 are separated by 133 nucleotides GFF1018 and GFF1019 are separated by 282 nucleotides GFF1019 and GFF1020 are separated by 57 nucleotides
GFF1017 - Putative glucosamine-fructose-6-phosphate aminotransferase, at 201,907 to 202,917
GFF1017
GFF1018 - Phosphoglycerol transferase I (EC 2.7.8.20), at 203,051 to 205,342
GFF1018
GFF1019 - Putative glycoprotein/receptor, at 205,625 to 206,086
GFF1019
GFF1020 - DNA replication protein DnaC, at 206,144 to 206,881
GFF1020
Position (kb) 203
204
205
206 Strain fitness (log2 ratio) -2
-1
0
1
2 at 202.131 kb on + strand, within GFF1017 at 202.170 kb on + strand, within GFF1017 at 202.338 kb on + strand, within GFF1017 at 202.338 kb on + strand, within GFF1017 at 202.339 kb on - strand, within GFF1017 at 202.339 kb on - strand, within GFF1017 at 202.349 kb on - strand, within GFF1017 at 202.509 kb on + strand, within GFF1017 at 202.509 kb on + strand, within GFF1017 at 202.510 kb on - strand, within GFF1017 at 202.510 kb on - strand, within GFF1017 at 202.566 kb on + strand, within GFF1017 at 202.832 kb on + strand at 202.864 kb on + strand at 202.932 kb on + strand at 202.932 kb on + strand at 202.933 kb on - strand at 202.937 kb on + strand at 202.937 kb on + strand at 202.939 kb on + strand at 202.940 kb on - strand at 202.940 kb on - strand at 203.036 kb on - strand at 203.037 kb on + strand at 203.037 kb on + strand at 203.038 kb on - strand at 203.126 kb on - strand at 203.165 kb on + strand at 203.170 kb on + strand at 203.339 kb on - strand, within GFF1018 at 203.340 kb on + strand, within GFF1018 at 203.471 kb on - strand, within GFF1018 at 203.579 kb on - strand, within GFF1018 at 203.579 kb on - strand, within GFF1018 at 203.591 kb on + strand, within GFF1018 at 203.664 kb on - strand, within GFF1018 at 203.679 kb on + strand, within GFF1018 at 203.799 kb on - strand, within GFF1018 at 203.977 kb on + strand, within GFF1018 at 204.121 kb on + strand, within GFF1018 at 204.142 kb on + strand, within GFF1018 at 204.319 kb on + strand, within GFF1018 at 204.320 kb on - strand, within GFF1018 at 204.341 kb on - strand, within GFF1018 at 204.344 kb on + strand, within GFF1018 at 204.345 kb on - strand, within GFF1018 at 204.614 kb on - strand, within GFF1018 at 204.679 kb on + strand, within GFF1018 at 204.715 kb on - strand, within GFF1018 at 204.845 kb on - strand, within GFF1018 at 205.015 kb on - strand, within GFF1018 at 205.015 kb on - strand, within GFF1018 at 205.156 kb on + strand at 205.293 kb on + strand at 205.293 kb on + strand at 205.293 kb on + strand at 205.293 kb on + strand at 205.293 kb on + strand at 205.295 kb on + strand at 205.295 kb on + strand at 205.296 kb on - strand at 205.296 kb on - strand at 205.399 kb on + strand at 205.402 kb on + strand at 205.502 kb on + strand at 205.774 kb on + strand, within GFF1019 at 205.900 kb on + strand, within GFF1019 at 205.901 kb on - strand, within GFF1019 at 206.040 kb on - strand at 206.040 kb on - strand at 206.080 kb on + strand at 206.080 kb on + strand at 206.081 kb on - strand at 206.090 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 1.875 MOI remove 202,131 + GFF1017 0.22 +0.1 202,170 + GFF1017 0.26 -0.5 202,338 + GFF1017 0.43 +0.0 202,338 + GFF1017 0.43 +0.4 202,339 - GFF1017 0.43 -0.3 202,339 - GFF1017 0.43 -1.2 202,349 - GFF1017 0.44 +0.2 202,509 + GFF1017 0.60 +0.5 202,509 + GFF1017 0.60 +0.1 202,510 - GFF1017 0.60 +0.5 202,510 - GFF1017 0.60 -0.0 202,566 + GFF1017 0.65 -0.6 202,832 + -2.5 202,864 + -1.1 202,932 + +0.3 202,932 + -0.1 202,933 - +0.6 202,937 + +0.4 202,937 + +0.3 202,939 + -0.1 202,940 - +0.1 202,940 - -0.2 203,036 - +1.7 203,037 + +0.1 203,037 + +0.1 203,038 - -0.8 203,126 - -0.2 203,165 + -0.2 203,170 + -0.6 203,339 - GFF1018 0.13 +0.7 203,340 + GFF1018 0.13 +0.2 203,471 - GFF1018 0.18 -0.0 203,579 - GFF1018 0.23 +0.2 203,579 - GFF1018 0.23 +1.4 203,591 + GFF1018 0.24 +0.1 203,664 - GFF1018 0.27 +0.5 203,679 + GFF1018 0.27 +0.0 203,799 - GFF1018 0.33 +0.8 203,977 + GFF1018 0.40 +0.1 204,121 + GFF1018 0.47 -0.3 204,142 + GFF1018 0.48 +2.3 204,319 + GFF1018 0.55 -0.2 204,320 - GFF1018 0.55 +0.4 204,341 - GFF1018 0.56 +0.1 204,344 + GFF1018 0.56 +0.1 204,345 - GFF1018 0.56 +0.2 204,614 - GFF1018 0.68 -0.3 204,679 + GFF1018 0.71 +0.1 204,715 - GFF1018 0.73 +0.9 204,845 - GFF1018 0.78 +0.5 205,015 - GFF1018 0.86 +0.4 205,015 - GFF1018 0.86 +1.6 205,156 + +0.5 205,293 + +0.1 205,293 + +0.1 205,293 + -0.3 205,293 + +1.3 205,293 + +0.3 205,295 + -1.1 205,295 + -0.2 205,296 - +0.2 205,296 - +0.0 205,399 + -0.3 205,402 + -0.3 205,502 + -0.1 205,774 + GFF1019 0.32 +0.2 205,900 + GFF1019 0.60 +0.4 205,901 - GFF1019 0.60 +0.1 206,040 - -0.8 206,040 - -0.3 206,080 + +0.6 206,080 + -0.4 206,081 - -0.2 206,090 - +0.2
Or see this region's nucleotide sequence