Strain Fitness in Escherichia coli BL21 around ECD_00190

Experiment: Mixed culture, Halobacteriovorax-10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_00187 and ECD_00188 overlap by 8 nucleotidesECD_00188 and ECD_00189 are separated by 165 nucleotidesECD_00189 and ECD_00190 overlap by 4 nucleotidesECD_00190 and ECD_00191 are separated by 13 nucleotidesECD_00191 and ECD_00192 are separated by 199 nucleotides ECD_00187: ECD_00187 - modulator of Rho-dependent transcription termination, at 216,521 to 216,775 _00187 ECD_00188: ECD_00188 - UPF0253 family protein, at 216,768 to 216,968 _00188 ECD_00189: ECD_00189 - PDDEXK superfamily protein, at 217,134 to 217,679 _00189 ECD_00190: ECD_00190 - alternative stalled-ribosome rescue factor B; peptidyl-tRNA hydrolase, ribosome-attached, at 217,676 to 218,098 _00190 ECD_00191: ECD_00191 - lipoprotein involved with copper homeostasis and adhesion, at 218,112 to 218,822 _00191 ECD_00192: ECD_00192 - putative lipoprotein, at 219,022 to 219,846 _00192 Position (kb) 217 218 219Strain fitness (log2 ratio) -1 0 1 2at 216.704 kb on + strand, within ECD_00187at 216.705 kb on - strand, within ECD_00187at 216.739 kb on - strand, within ECD_00187at 216.739 kb on - strand, within ECD_00187at 216.763 kb on - strandat 216.767 kb on - strandat 216.767 kb on - strandat 216.811 kb on + strand, within ECD_00188at 216.812 kb on - strand, within ECD_00188at 216.910 kb on - strand, within ECD_00188at 216.910 kb on - strand, within ECD_00188at 216.959 kb on - strandat 217.012 kb on + strandat 217.049 kb on + strandat 217.050 kb on - strandat 217.050 kb on - strandat 217.051 kb on + strandat 217.051 kb on + strandat 217.051 kb on + strandat 217.051 kb on + strandat 217.052 kb on - strandat 217.052 kb on - strandat 217.052 kb on - strandat 217.052 kb on - strandat 217.093 kb on - strandat 217.250 kb on + strand, within ECD_00189at 217.251 kb on - strand, within ECD_00189at 217.281 kb on + strand, within ECD_00189at 217.281 kb on + strand, within ECD_00189at 217.452 kb on + strand, within ECD_00189at 217.452 kb on + strand, within ECD_00189at 217.452 kb on + strand, within ECD_00189at 217.452 kb on + strand, within ECD_00189at 217.453 kb on - strand, within ECD_00189at 217.453 kb on - strand, within ECD_00189at 217.454 kb on + strand, within ECD_00189at 217.454 kb on + strand, within ECD_00189at 217.638 kb on - strandat 217.769 kb on - strand, within ECD_00190at 217.826 kb on + strand, within ECD_00190at 217.827 kb on - strand, within ECD_00190at 217.829 kb on + strand, within ECD_00190at 217.829 kb on + strand, within ECD_00190at 217.968 kb on + strand, within ECD_00190at 217.968 kb on + strand, within ECD_00190at 218.159 kb on + strandat 218.159 kb on + strandat 218.174 kb on + strandat 218.177 kb on + strandat 218.373 kb on + strand, within ECD_00191at 218.378 kb on + strand, within ECD_00191at 218.508 kb on + strand, within ECD_00191at 218.509 kb on - strand, within ECD_00191at 218.511 kb on - strand, within ECD_00191at 218.536 kb on + strand, within ECD_00191at 218.536 kb on + strand, within ECD_00191at 218.537 kb on - strand, within ECD_00191at 218.540 kb on + strand, within ECD_00191at 218.568 kb on + strand, within ECD_00191at 218.574 kb on + strand, within ECD_00191at 218.575 kb on - strand, within ECD_00191at 218.576 kb on + strand, within ECD_00191at 218.695 kb on + strand, within ECD_00191at 218.704 kb on + strand, within ECD_00191at 218.718 kb on - strand, within ECD_00191at 218.759 kb on - strandat 218.969 kb on + strandat 218.970 kb on - strandat 218.978 kb on + strandat 218.978 kb on + strandat 218.993 kb on - strandat 219.015 kb on + strandat 219.016 kb on - strandat 219.059 kb on + strandat 219.059 kb on + strandat 219.060 kb on - strandat 219.086 kb on + strandat 219.087 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Mixed culture, Halobacteriovorax-10
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216,704 + ECD_00187 0.72 +0.3
216,705 - ECD_00187 0.72 +0.0
216,739 - ECD_00187 0.85 +0.0
216,739 - ECD_00187 0.85 -0.5
216,763 - +0.3
216,767 - -0.2
216,767 - +0.1
216,811 + ECD_00188 0.21 +0.1
216,812 - ECD_00188 0.22 -0.0
216,910 - ECD_00188 0.71 +0.2
216,910 - ECD_00188 0.71 +0.5
216,959 - -0.3
217,012 + -0.6
217,049 + +0.5
217,050 - -0.1
217,050 - -0.8
217,051 + +0.1
217,051 + +0.2
217,051 + -0.9
217,051 + +0.5
217,052 - +0.3
217,052 - +0.1
217,052 - -0.1
217,052 - +0.3
217,093 - -0.9
217,250 + ECD_00189 0.21 -1.5
217,251 - ECD_00189 0.21 +0.1
217,281 + ECD_00189 0.27 -0.9
217,281 + ECD_00189 0.27 +2.3
217,452 + ECD_00189 0.58 +0.3
217,452 + ECD_00189 0.58 +0.0
217,452 + ECD_00189 0.58 +0.1
217,452 + ECD_00189 0.58 -0.4
217,453 - ECD_00189 0.58 +0.1
217,453 - ECD_00189 0.58 -0.9
217,454 + ECD_00189 0.59 -0.1
217,454 + ECD_00189 0.59 -0.1
217,638 - -0.7
217,769 - ECD_00190 0.22 +0.3
217,826 + ECD_00190 0.35 -0.0
217,827 - ECD_00190 0.36 +0.1
217,829 + ECD_00190 0.36 +0.6
217,829 + ECD_00190 0.36 +0.6
217,968 + ECD_00190 0.69 +0.4
217,968 + ECD_00190 0.69 +0.2
218,159 + -0.2
218,159 + +0.0
218,174 + +0.5
218,177 + +0.0
218,373 + ECD_00191 0.37 -0.1
218,378 + ECD_00191 0.37 -0.7
218,508 + ECD_00191 0.56 +0.5
218,509 - ECD_00191 0.56 -1.1
218,511 - ECD_00191 0.56 -1.3
218,536 + ECD_00191 0.60 -0.2
218,536 + ECD_00191 0.60 -0.0
218,537 - ECD_00191 0.60 -0.3
218,540 + ECD_00191 0.60 +0.3
218,568 + ECD_00191 0.64 -0.2
218,574 + ECD_00191 0.65 -0.2
218,575 - ECD_00191 0.65 +0.0
218,576 + ECD_00191 0.65 -0.2
218,695 + ECD_00191 0.82 -0.3
218,704 + ECD_00191 0.83 -0.3
218,718 - ECD_00191 0.85 +1.0
218,759 - +0.3
218,969 + -1.4
218,970 - +0.2
218,978 + -1.6
218,978 + -0.4
218,993 - -0.4
219,015 + -0.2
219,016 - -0.4
219,059 + -0.0
219,059 + +1.0
219,060 - +0.5
219,086 + -0.3
219,087 - +0.1

Or see this region's nucleotide sequence