Experiment: R2A with Strontium chloride 750 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu5526 and Atu5527 are separated by 22 nucleotides Atu5527 and Atu5528 are separated by 739 nucleotides
Atu5526: Atu5526 - monooxygenase, at 518,135 to 519,238
Atu5526
Atu5527: Atu5527 - NADH-dependent FMN reductase, at 519,261 to 519,863
Atu5527
Atu5528: Atu5528 - two component sensor kinase, at 520,603 to 522,531
Atu5528
Position (kb) 519
520 Strain fitness (log2 ratio) -2
-1
0
1
2 at 518.270 kb on + strand, within Atu5526 at 518.307 kb on + strand, within Atu5526 at 518.317 kb on + strand, within Atu5526 at 518.320 kb on - strand, within Atu5526 at 518.320 kb on - strand, within Atu5526 at 518.325 kb on - strand, within Atu5526 at 518.430 kb on + strand, within Atu5526 at 518.438 kb on - strand, within Atu5526 at 518.478 kb on + strand, within Atu5526 at 518.486 kb on - strand, within Atu5526 at 518.486 kb on - strand, within Atu5526 at 518.518 kb on - strand, within Atu5526 at 518.536 kb on - strand, within Atu5526 at 518.536 kb on - strand, within Atu5526 at 518.550 kb on + strand, within Atu5526 at 518.567 kb on + strand, within Atu5526 at 518.585 kb on + strand, within Atu5526 at 518.593 kb on + strand, within Atu5526 at 518.601 kb on - strand, within Atu5526 at 518.601 kb on - strand, within Atu5526 at 518.653 kb on - strand, within Atu5526 at 518.694 kb on - strand, within Atu5526 at 518.709 kb on - strand, within Atu5526 at 518.709 kb on - strand, within Atu5526 at 518.805 kb on + strand, within Atu5526 at 518.805 kb on - strand, within Atu5526 at 518.834 kb on + strand, within Atu5526 at 518.909 kb on - strand, within Atu5526 at 518.937 kb on - strand, within Atu5526 at 518.964 kb on - strand, within Atu5526 at 518.973 kb on + strand, within Atu5526 at 518.973 kb on - strand, within Atu5526 at 519.068 kb on + strand, within Atu5526 at 519.125 kb on + strand, within Atu5526 at 519.260 kb on - strand at 519.384 kb on - strand, within Atu5527 at 519.396 kb on - strand, within Atu5527 at 519.426 kb on + strand, within Atu5527 at 519.433 kb on + strand, within Atu5527 at 519.454 kb on - strand, within Atu5527 at 519.473 kb on - strand, within Atu5527 at 519.483 kb on - strand, within Atu5527 at 519.499 kb on + strand, within Atu5527 at 519.501 kb on + strand, within Atu5527 at 519.509 kb on + strand, within Atu5527 at 519.554 kb on - strand, within Atu5527 at 519.659 kb on + strand, within Atu5527 at 519.702 kb on - strand, within Atu5527 at 519.703 kb on + strand, within Atu5527 at 519.711 kb on - strand, within Atu5527 at 519.711 kb on - strand, within Atu5527 at 519.732 kb on - strand, within Atu5527 at 519.743 kb on + strand, within Atu5527 at 519.851 kb on + strand at 519.972 kb on + strand at 519.980 kb on - strand at 519.996 kb on - strand at 520.053 kb on + strand at 520.061 kb on - strand at 520.079 kb on + strand at 520.087 kb on - strand at 520.087 kb on - strand at 520.087 kb on - strand at 520.276 kb on - strand at 520.280 kb on - strand at 520.339 kb on + strand at 520.349 kb on + strand at 520.349 kb on + strand at 520.357 kb on - strand at 520.736 kb on + strand at 520.741 kb on - strand at 520.761 kb on - strand at 520.772 kb on + strand at 520.772 kb on + strand at 520.772 kb on + strand at 520.780 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Strontium chloride 750 mM remove 518,270 + Atu5526 0.12 +0.7 518,307 + Atu5526 0.16 -0.3 518,317 + Atu5526 0.16 +0.7 518,320 - Atu5526 0.17 +1.5 518,320 - Atu5526 0.17 -0.3 518,325 - Atu5526 0.17 +0.1 518,430 + Atu5526 0.27 -0.0 518,438 - Atu5526 0.27 -0.7 518,478 + Atu5526 0.31 +0.2 518,486 - Atu5526 0.32 +0.7 518,486 - Atu5526 0.32 -0.2 518,518 - Atu5526 0.35 +0.5 518,536 - Atu5526 0.36 -0.2 518,536 - Atu5526 0.36 -0.5 518,550 + Atu5526 0.38 -0.4 518,567 + Atu5526 0.39 +0.0 518,585 + Atu5526 0.41 +0.2 518,593 + Atu5526 0.41 +2.5 518,601 - Atu5526 0.42 -0.2 518,601 - Atu5526 0.42 -0.5 518,653 - Atu5526 0.47 -0.2 518,694 - Atu5526 0.51 +0.0 518,709 - Atu5526 0.52 -1.3 518,709 - Atu5526 0.52 +0.8 518,805 + Atu5526 0.61 -2.2 518,805 - Atu5526 0.61 -0.2 518,834 + Atu5526 0.63 +0.3 518,909 - Atu5526 0.70 -0.0 518,937 - Atu5526 0.73 +2.4 518,964 - Atu5526 0.75 +0.1 518,973 + Atu5526 0.76 +1.3 518,973 - Atu5526 0.76 -0.2 519,068 + Atu5526 0.85 -1.0 519,125 + Atu5526 0.90 -0.2 519,260 - -0.2 519,384 - Atu5527 0.20 -1.3 519,396 - Atu5527 0.22 +1.2 519,426 + Atu5527 0.27 +0.2 519,433 + Atu5527 0.29 -0.0 519,454 - Atu5527 0.32 -1.3 519,473 - Atu5527 0.35 +0.0 519,483 - Atu5527 0.37 -0.2 519,499 + Atu5527 0.39 -0.4 519,501 + Atu5527 0.40 +0.1 519,509 + Atu5527 0.41 -1.8 519,554 - Atu5527 0.49 +0.2 519,659 + Atu5527 0.66 +1.6 519,702 - Atu5527 0.73 -0.2 519,703 + Atu5527 0.73 -0.5 519,711 - Atu5527 0.75 +0.4 519,711 - Atu5527 0.75 -0.3 519,732 - Atu5527 0.78 -0.4 519,743 + Atu5527 0.80 +0.2 519,851 + +0.2 519,972 + +0.3 519,980 - +0.6 519,996 - -0.7 520,053 + +0.2 520,061 - -0.7 520,079 + +0.5 520,087 - +0.1 520,087 - -0.6 520,087 - -0.4 520,276 - +0.2 520,280 - +0.4 520,339 + +1.3 520,349 + -0.0 520,349 + +0.1 520,357 - +0.0 520,736 + -0.1 520,741 - -0.2 520,761 - +0.5 520,772 + -0.0 520,772 + -0.4 520,772 + +2.2 520,780 - -0.1
Or see this region's nucleotide sequence