Experiment: R2A with Strontium chloride 750 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu5121 and Atu5122 are separated by 38 nucleotides Atu5122 and Atu5123 overlap by 4 nucleotides Atu5123 and Atu5124 overlap by 1 nucleotides
Atu5121: Atu5121 - two component response regulator, at 127,712 to 128,386
Atu5121
Atu5122: Atu5122 - 3-ketoacyl-ACP reductase, at 128,425 to 129,204
Atu5122
Atu5123: Atu5123 - acetolactate synthase catalytic subunit, at 129,201 to 130,916
Atu5123
Atu5124: Atu5124 - glutamate-1-semialdehyde aminotransferase, at 130,916 to 132,250
Atu5124
Position (kb)
129
130
131 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 128.214 kb on + strand, within Atu5121 at 128.217 kb on - strand, within Atu5121 at 128.219 kb on + strand, within Atu5121 at 128.219 kb on + strand, within Atu5121 at 128.219 kb on + strand, within Atu5121 at 128.222 kb on - strand, within Atu5121 at 128.227 kb on - strand, within Atu5121 at 128.227 kb on - strand, within Atu5121 at 128.227 kb on - strand, within Atu5121 at 128.227 kb on - strand, within Atu5121 at 128.227 kb on - strand, within Atu5121 at 128.227 kb on - strand, within Atu5121 at 128.232 kb on - strand, within Atu5121 at 128.273 kb on + strand, within Atu5121 at 128.337 kb on - strand at 128.368 kb on - strand at 128.368 kb on - strand at 128.432 kb on + strand at 128.432 kb on + strand at 128.436 kb on - strand at 128.532 kb on + strand, within Atu5122 at 128.532 kb on + strand, within Atu5122 at 128.540 kb on - strand, within Atu5122 at 128.542 kb on + strand, within Atu5122 at 128.677 kb on + strand, within Atu5122 at 128.682 kb on + strand, within Atu5122 at 128.685 kb on - strand, within Atu5122 at 128.685 kb on - strand, within Atu5122 at 128.685 kb on - strand, within Atu5122 at 128.685 kb on - strand, within Atu5122 at 128.722 kb on + strand, within Atu5122 at 128.736 kb on + strand, within Atu5122 at 128.763 kb on + strand, within Atu5122 at 128.766 kb on - strand, within Atu5122 at 128.810 kb on + strand, within Atu5122 at 128.816 kb on - strand, within Atu5122 at 128.818 kb on + strand, within Atu5122 at 128.885 kb on + strand, within Atu5122 at 128.940 kb on + strand, within Atu5122 at 128.967 kb on - strand, within Atu5122 at 129.038 kb on - strand, within Atu5122 at 129.038 kb on - strand, within Atu5122 at 129.047 kb on + strand, within Atu5122 at 129.070 kb on - strand, within Atu5122 at 129.111 kb on + strand, within Atu5122 at 129.211 kb on + strand at 129.216 kb on + strand at 129.255 kb on - strand at 129.259 kb on + strand at 129.259 kb on + strand at 129.267 kb on - strand at 129.313 kb on + strand at 129.328 kb on - strand at 129.370 kb on + strand at 129.378 kb on - strand, within Atu5123 at 129.414 kb on - strand, within Atu5123 at 129.414 kb on - strand, within Atu5123 at 129.414 kb on - strand, within Atu5123 at 129.450 kb on - strand, within Atu5123 at 129.520 kb on + strand, within Atu5123 at 129.528 kb on - strand, within Atu5123 at 129.528 kb on - strand, within Atu5123 at 129.528 kb on - strand, within Atu5123 at 129.544 kb on + strand, within Atu5123 at 129.544 kb on + strand, within Atu5123 at 129.552 kb on - strand, within Atu5123 at 129.552 kb on - strand, within Atu5123 at 129.552 kb on - strand, within Atu5123 at 129.575 kb on - strand, within Atu5123 at 129.623 kb on + strand, within Atu5123 at 129.623 kb on - strand, within Atu5123 at 129.631 kb on - strand, within Atu5123 at 129.769 kb on + strand, within Atu5123 at 129.785 kb on + strand, within Atu5123 at 129.791 kb on - strand, within Atu5123 at 129.795 kb on + strand, within Atu5123 at 129.795 kb on + strand, within Atu5123 at 129.795 kb on + strand, within Atu5123 at 129.803 kb on - strand, within Atu5123 at 129.854 kb on - strand, within Atu5123 at 129.893 kb on + strand, within Atu5123 at 129.893 kb on + strand, within Atu5123 at 129.993 kb on - strand, within Atu5123 at 130.020 kb on - strand, within Atu5123 at 130.020 kb on - strand, within Atu5123 at 130.044 kb on - strand, within Atu5123 at 130.126 kb on - strand, within Atu5123 at 130.174 kb on + strand, within Atu5123 at 130.182 kb on - strand, within Atu5123 at 130.215 kb on - strand, within Atu5123 at 130.247 kb on - strand, within Atu5123 at 130.249 kb on - strand, within Atu5123 at 130.351 kb on - strand, within Atu5123 at 130.405 kb on - strand, within Atu5123 at 130.545 kb on - strand, within Atu5123 at 130.598 kb on - strand, within Atu5123 at 130.634 kb on + strand, within Atu5123 at 130.647 kb on - strand, within Atu5123 at 130.656 kb on - strand, within Atu5123 at 130.687 kb on - strand, within Atu5123 at 130.689 kb on + strand, within Atu5123 at 130.697 kb on - strand, within Atu5123 at 130.697 kb on - strand, within Atu5123 at 130.735 kb on + strand, within Atu5123 at 130.789 kb on - strand at 130.789 kb on - strand at 130.789 kb on - strand at 130.986 kb on + strand at 131.072 kb on - strand, within Atu5124 at 131.210 kb on + strand, within Atu5124 at 131.210 kb on + strand, within Atu5124 at 131.218 kb on - strand, within Atu5124 at 131.218 kb on - strand, within Atu5124 at 131.275 kb on - strand, within Atu5124 at 131.325 kb on + strand, within Atu5124 at 131.325 kb on + strand, within Atu5124 at 131.383 kb on + strand, within Atu5124 at 131.389 kb on - strand, within Atu5124 at 131.429 kb on - strand, within Atu5124 at 131.449 kb on - strand, within Atu5124 at 131.506 kb on - strand, within Atu5124 at 131.506 kb on - strand, within Atu5124 at 131.506 kb on - strand, within Atu5124 at 131.549 kb on + strand, within Atu5124 at 131.567 kb on + strand, within Atu5124 at 131.569 kb on + strand, within Atu5124 at 131.577 kb on - strand, within Atu5124 at 131.579 kb on - strand, within Atu5124 at 131.590 kb on + strand, within Atu5124 at 131.590 kb on - strand, within Atu5124 at 131.777 kb on + strand, within Atu5124 at 131.777 kb on + strand, within Atu5124 at 131.777 kb on + strand, within Atu5124 at 131.777 kb on + strand, within Atu5124 at 131.785 kb on - strand, within Atu5124 at 131.785 kb on - strand, within Atu5124 at 131.785 kb on - strand, within Atu5124 at 131.785 kb on - strand, within Atu5124 at 131.785 kb on - strand, within Atu5124 at 131.799 kb on + strand, within Atu5124 at 131.799 kb on + strand, within Atu5124 at 131.799 kb on + strand, within Atu5124 at 131.799 kb on + strand, within Atu5124 at 131.807 kb on - strand, within Atu5124 at 131.807 kb on - strand, within Atu5124 at 131.807 kb on - strand, within Atu5124 at 131.807 kb on - strand, within Atu5124 at 131.881 kb on + strand, within Atu5124 at 131.894 kb on + strand, within Atu5124
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Strontium chloride 750 mM remove 128,214 + Atu5121 0.74 -1.3 128,217 - Atu5121 0.75 -0.7 128,219 + Atu5121 0.75 -1.2 128,219 + Atu5121 0.75 -0.7 128,219 + Atu5121 0.75 +0.4 128,222 - Atu5121 0.76 -0.5 128,227 - Atu5121 0.76 -1.0 128,227 - Atu5121 0.76 +0.2 128,227 - Atu5121 0.76 -0.3 128,227 - Atu5121 0.76 -1.0 128,227 - Atu5121 0.76 -1.3 128,227 - Atu5121 0.76 +0.1 128,232 - Atu5121 0.77 -0.4 128,273 + Atu5121 0.83 +0.5 128,337 - -0.3 128,368 - +0.7 128,368 - -0.0 128,432 + +0.1 128,432 + -1.0 128,436 - +0.3 128,532 + Atu5122 0.14 +0.1 128,532 + Atu5122 0.14 -1.6 128,540 - Atu5122 0.15 +0.5 128,542 + Atu5122 0.15 -0.4 128,677 + Atu5122 0.32 -1.3 128,682 + Atu5122 0.33 +0.5 128,685 - Atu5122 0.33 -1.5 128,685 - Atu5122 0.33 -0.2 128,685 - Atu5122 0.33 +0.6 128,685 - Atu5122 0.33 +0.6 128,722 + Atu5122 0.38 +0.1 128,736 + Atu5122 0.40 +0.7 128,763 + Atu5122 0.43 -0.4 128,766 - Atu5122 0.44 +1.0 128,810 + Atu5122 0.49 +0.8 128,816 - Atu5122 0.50 -0.2 128,818 + Atu5122 0.50 +0.2 128,885 + Atu5122 0.59 +0.7 128,940 + Atu5122 0.66 +0.2 128,967 - Atu5122 0.69 -0.9 129,038 - Atu5122 0.79 -1.4 129,038 - Atu5122 0.79 -0.0 129,047 + Atu5122 0.80 +2.2 129,070 - Atu5122 0.83 +0.5 129,111 + Atu5122 0.88 -0.5 129,211 + +0.7 129,216 + +0.8 129,255 - +0.9 129,259 + -0.1 129,259 + -1.8 129,267 - +0.8 129,313 + -1.2 129,328 - -0.4 129,370 + -0.8 129,378 - Atu5123 0.10 -0.1 129,414 - Atu5123 0.12 +0.5 129,414 - Atu5123 0.12 -0.2 129,414 - Atu5123 0.12 -1.1 129,450 - Atu5123 0.15 -0.3 129,520 + Atu5123 0.19 -0.4 129,528 - Atu5123 0.19 -0.4 129,528 - Atu5123 0.19 -0.7 129,528 - Atu5123 0.19 +0.8 129,544 + Atu5123 0.20 +1.0 129,544 + Atu5123 0.20 +1.8 129,552 - Atu5123 0.20 -0.6 129,552 - Atu5123 0.20 -0.1 129,552 - Atu5123 0.20 -0.6 129,575 - Atu5123 0.22 -0.0 129,623 + Atu5123 0.25 -0.5 129,623 - Atu5123 0.25 +0.8 129,631 - Atu5123 0.25 -0.5 129,769 + Atu5123 0.33 +0.2 129,785 + Atu5123 0.34 -0.3 129,791 - Atu5123 0.34 -0.3 129,795 + Atu5123 0.35 -0.6 129,795 + Atu5123 0.35 -0.1 129,795 + Atu5123 0.35 -0.5 129,803 - Atu5123 0.35 +0.2 129,854 - Atu5123 0.38 +0.4 129,893 + Atu5123 0.40 -0.3 129,893 + Atu5123 0.40 -0.1 129,993 - Atu5123 0.46 +0.4 130,020 - Atu5123 0.48 +0.0 130,020 - Atu5123 0.48 -0.3 130,044 - Atu5123 0.49 +0.2 130,126 - Atu5123 0.54 -0.5 130,174 + Atu5123 0.57 -0.1 130,182 - Atu5123 0.57 -1.0 130,215 - Atu5123 0.59 -1.0 130,247 - Atu5123 0.61 -0.7 130,249 - Atu5123 0.61 -0.3 130,351 - Atu5123 0.67 +0.2 130,405 - Atu5123 0.70 -0.6 130,545 - Atu5123 0.78 -0.0 130,598 - Atu5123 0.81 +0.1 130,634 + Atu5123 0.84 -0.8 130,647 - Atu5123 0.84 -0.0 130,656 - Atu5123 0.85 +0.3 130,687 - Atu5123 0.87 -0.5 130,689 + Atu5123 0.87 +0.3 130,697 - Atu5123 0.87 -0.7 130,697 - Atu5123 0.87 +1.8 130,735 + Atu5123 0.89 -0.7 130,789 - -0.0 130,789 - -0.9 130,789 - +0.2 130,986 + +0.1 131,072 - Atu5124 0.12 -1.3 131,210 + Atu5124 0.22 -0.1 131,210 + Atu5124 0.22 +0.5 131,218 - Atu5124 0.23 -0.9 131,218 - Atu5124 0.23 +0.5 131,275 - Atu5124 0.27 -0.5 131,325 + Atu5124 0.31 -2.0 131,325 + Atu5124 0.31 -0.2 131,383 + Atu5124 0.35 -1.0 131,389 - Atu5124 0.35 -2.0 131,429 - Atu5124 0.38 -0.6 131,449 - Atu5124 0.40 -0.6 131,506 - Atu5124 0.44 -0.4 131,506 - Atu5124 0.44 +2.3 131,506 - Atu5124 0.44 -0.8 131,549 + Atu5124 0.47 +0.0 131,567 + Atu5124 0.49 +0.3 131,569 + Atu5124 0.49 +0.1 131,577 - Atu5124 0.50 -0.9 131,579 - Atu5124 0.50 -1.1 131,590 + Atu5124 0.50 +0.6 131,590 - Atu5124 0.50 -0.1 131,777 + Atu5124 0.64 -0.6 131,777 + Atu5124 0.64 -0.0 131,777 + Atu5124 0.64 +0.2 131,777 + Atu5124 0.64 -0.4 131,785 - Atu5124 0.65 -0.5 131,785 - Atu5124 0.65 -0.9 131,785 - Atu5124 0.65 -0.4 131,785 - Atu5124 0.65 -3.0 131,785 - Atu5124 0.65 -2.7 131,799 + Atu5124 0.66 -0.8 131,799 + Atu5124 0.66 -0.5 131,799 + Atu5124 0.66 -0.5 131,799 + Atu5124 0.66 -0.8 131,807 - Atu5124 0.67 -0.8 131,807 - Atu5124 0.67 -0.8 131,807 - Atu5124 0.67 +0.8 131,807 - Atu5124 0.67 -2.1 131,881 + Atu5124 0.72 -1.2 131,894 + Atu5124 0.73 -1.1
Or see this region's nucleotide sequence