Experiment: R2A with Strontium chloride 750 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu0297 and Atu0298 are separated by 20 nucleotides Atu0298 and Atu0299 are separated by 4 nucleotides Atu0299 and Atu0300 are separated by 96 nucleotides Atu0300 and Atu0301 are separated by 193 nucleotides
Atu0297: Atu0297 - hypothetical protein, at 291,257 to 291,544
Atu0297
Atu0298: Atu0298 - orotidine 5, at 291,565 to 292,272
Atu0298
Atu0299: Atu0299 - hypothetical protein, at 292,277 to 292,873
Atu0299
Atu0300: Atu0300 - methyltransferase, at 292,970 to 293,563
Atu0300
Atu0301: Atu0301 - DNA polymerase III, beta chain, at 293,757 to 294,875
Atu0301
Position (kb) 292
293 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 291.323 kb on + strand, within Atu0297 at 291.333 kb on - strand, within Atu0297 at 291.348 kb on + strand, within Atu0297 at 291.363 kb on + strand, within Atu0297 at 291.454 kb on + strand, within Atu0297 at 291.471 kb on + strand, within Atu0297 at 291.859 kb on + strand, within Atu0298 at 291.862 kb on - strand, within Atu0298 at 291.904 kb on - strand, within Atu0298 at 291.925 kb on + strand, within Atu0298 at 291.933 kb on - strand, within Atu0298 at 291.933 kb on - strand, within Atu0298 at 291.993 kb on - strand, within Atu0298 at 292.000 kb on - strand, within Atu0298 at 292.022 kb on + strand, within Atu0298 at 292.064 kb on + strand, within Atu0298 at 292.072 kb on - strand, within Atu0298 at 292.072 kb on - strand, within Atu0298 at 292.074 kb on - strand, within Atu0298 at 292.083 kb on + strand, within Atu0298 at 292.182 kb on + strand, within Atu0298 at 292.210 kb on + strand at 292.227 kb on - strand at 292.268 kb on + strand at 292.274 kb on + strand at 292.275 kb on - strand at 292.409 kb on + strand, within Atu0299 at 292.421 kb on + strand, within Atu0299 at 292.422 kb on + strand, within Atu0299 at 292.461 kb on - strand, within Atu0299 at 292.471 kb on + strand, within Atu0299 at 292.479 kb on - strand, within Atu0299 at 292.489 kb on - strand, within Atu0299 at 292.555 kb on - strand, within Atu0299 at 292.691 kb on + strand, within Atu0299 at 292.746 kb on + strand, within Atu0299 at 292.755 kb on - strand, within Atu0299 at 292.757 kb on + strand, within Atu0299 at 292.825 kb on - strand at 292.836 kb on + strand at 292.842 kb on + strand at 292.852 kb on + strand at 292.862 kb on - strand at 292.910 kb on - strand at 292.910 kb on - strand at 292.986 kb on + strand at 293.054 kb on - strand, within Atu0300 at 293.108 kb on - strand, within Atu0300 at 293.186 kb on + strand, within Atu0300 at 293.193 kb on - strand, within Atu0300 at 293.201 kb on + strand, within Atu0300 at 293.208 kb on + strand, within Atu0300 at 293.216 kb on - strand, within Atu0300 at 293.285 kb on - strand, within Atu0300 at 293.302 kb on - strand, within Atu0300 at 293.330 kb on + strand, within Atu0300 at 293.366 kb on + strand, within Atu0300 at 293.492 kb on + strand, within Atu0300 at 293.545 kb on + strand at 293.553 kb on - strand at 293.553 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Strontium chloride 750 mM remove 291,323 + Atu0297 0.23 -0.8 291,333 - Atu0297 0.26 -0.1 291,348 + Atu0297 0.32 +0.9 291,363 + Atu0297 0.37 +0.5 291,454 + Atu0297 0.68 -1.3 291,471 + Atu0297 0.74 -0.1 291,859 + Atu0298 0.42 -0.3 291,862 - Atu0298 0.42 +0.5 291,904 - Atu0298 0.48 -0.9 291,925 + Atu0298 0.51 -1.5 291,933 - Atu0298 0.52 -2.1 291,933 - Atu0298 0.52 -1.0 291,993 - Atu0298 0.60 -1.3 292,000 - Atu0298 0.61 -3.8 292,022 + Atu0298 0.65 -1.1 292,064 + Atu0298 0.70 -0.5 292,072 - Atu0298 0.72 -0.6 292,072 - Atu0298 0.72 +0.9 292,074 - Atu0298 0.72 -1.9 292,083 + Atu0298 0.73 -1.3 292,182 + Atu0298 0.87 +0.4 292,210 + -1.1 292,227 - -1.2 292,268 + +1.2 292,274 + -0.0 292,275 - +0.6 292,409 + Atu0299 0.22 -0.6 292,421 + Atu0299 0.24 +0.3 292,422 + Atu0299 0.24 +0.1 292,461 - Atu0299 0.31 +0.2 292,471 + Atu0299 0.32 +1.0 292,479 - Atu0299 0.34 -0.5 292,489 - Atu0299 0.36 +0.2 292,555 - Atu0299 0.47 +0.1 292,691 + Atu0299 0.69 +0.6 292,746 + Atu0299 0.79 +0.5 292,755 - Atu0299 0.80 +1.2 292,757 + Atu0299 0.80 -0.4 292,825 - +0.6 292,836 + +0.2 292,842 + +0.2 292,852 + -0.5 292,862 - -0.3 292,910 - +0.2 292,910 - +0.4 292,986 + +0.1 293,054 - Atu0300 0.14 +2.2 293,108 - Atu0300 0.23 +1.2 293,186 + Atu0300 0.36 +0.9 293,193 - Atu0300 0.38 -0.1 293,201 + Atu0300 0.39 +0.1 293,208 + Atu0300 0.40 +1.3 293,216 - Atu0300 0.41 +0.9 293,285 - Atu0300 0.53 +1.3 293,302 - Atu0300 0.56 +1.3 293,330 + Atu0300 0.61 +0.1 293,366 + Atu0300 0.67 +0.3 293,492 + Atu0300 0.88 +1.6 293,545 + +0.4 293,553 - +0.2 293,553 - +0.4
Or see this region's nucleotide sequence